Effects of Selenium on DNA Methylation and Genomic Instability Induced by Drought Stress in Wheat (Triticum aestivum L.)

Zehra Şahin; Güleray Ağar; Esma Yiğider; Murat Aydın

Makale detayları

Effects of Selenium on DNA Methylation and Genomic Instability Induced by Drought Stress in Wheat (Triticum aestivum L.)

Yazarlar:

Zehra Şahin | Güleray Ağar | Esma Yiğider | Murat Aydın

Yayıncı:

Siirt University

Ana Başlıklar:

Bitki Biyoteknolojisi

Dergi:

Türkiye Tarımsal Araştırmalar Dergisi Vol.11 Issue 1, pp.26-37

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001315068

pISSN:

2148-2306

Anahtar kelimeler:

Özet:

 The main purpose of the study was to clarify the effect of selenium Se on DNA damage and DNA methylation in wheat Triticum aestivum L. plants exposed to polyethylene glycol PEG-induced drought stress under in vitro tissue culture. Random amplified polymorphic DNA RAPD and coupled restriction enzyme digestion-random amplification CRED-RA were utilized to explain the DNA damage grade and variations in DNA methylation patterns, respectively. The outcomes indicate that drought stress gives rise to a rise in RAPD profile variations as DNA damage and a decrease in genomic template stability GTS rate and DNA methylation changes. According to the RAPD data, the greatest GTS value was computed at 56.9 5 PEG 6000, and the lowest GTS value was 41.2 15 PEG 6000, demonstrating the adverse effects of PEG 6000. However, DNA damage can be reduced by treatment with sodium selenate 2, 4, and 6 µM of Na2SeO4 together with PEG 5, 10, and 15 PEG 6000-induced water deficits. Moreover, according to CRED-RA analysis, PEG-induced DNA methylation rates were changed after treating different doses of Se. These data demonstrate that Se dose-dependently modulates both DNA damage and methylation alterations induced by drought in wheat.

 The main purpose of the study was to clarify the effect of selenium Se on DNA damage and DNA methylation in wheat Triticum aestivum L. plants exposed to polyethylene glycol PEG-induced drought stress under in vitro tissue culture. Random amplified polymorphic DNA RAPD and coupled restriction enzyme digestion-random amplification CRED-RA were utilized to explain the DNA damage grade and variations in DNA methylation patterns, respectively. The outcomes indicate that drought stress gives rise to a rise in RAPD profile variations as DNA damage and a decrease in genomic template stability GTS rate and DNA methylation changes. According to the RAPD data, the greatest GTS value was computed at 56.9 5 PEG 6000, and the lowest GTS value was 41.2 15 PEG 6000, demonstrating the adverse effects of PEG 6000. However, DNA damage can be reduced by treatment with sodium selenate 2, 4, and 6 µM of Na2SeO4 together with PEG 5, 10, and 15 PEG 6000-induced water deficits. Moreover, according to CRED-RA analysis, PEG-induced DNA methylation rates were changed after treating different doses of Se. These data demonstrate that Se dose-dependently modulates both DNA damage and methylation alterations induced by drought in wheat.

 The main purpose of the study was to clarify the effect of selenium Se on DNA damage and DNA methylation in wheat Triticum aestivum L. plants exposed to polyethylene glycol PEG-induced drought stress under in vitro tissue culture. Random amplified polymorphic DNA RAPD and coupled restriction enzyme digestion-random amplification CRED-RA were utilized to explain the DNA damage grade and variations in DNA methylation patterns, respectively. The outcomes indicate that drought stress gives rise to a rise in RAPD profile variations as DNA damage and a decrease in genomic template stability GTS rate and DNA methylation changes. According to the RAPD data, the greatest GTS value was computed at 56.9 5 PEG 6000, and the lowest GTS value was 41.2 15 PEG 6000, demonstrating the adverse effects of PEG 6000. However, DNA damage can be reduced by treatment with sodium selenate 2, 4, and 6 µM of Na2SeO4 together with PEG 5, 10, and 15 PEG 6000-induced water deficits. Moreover, according to CRED-RA analysis, PEG-induced DNA methylation rates were changed after treating different doses of Se. These data demonstrate that Se dose-dependently modulates both DNA damage and methylation alterations induced by drought in wheat.

 The main purpose of the study was to clarify the effect of selenium Se on DNA damage and DNA methylation in wheat Triticum aestivum L. plants exposed to polyethylene glycol PEG-induced drought stress under in vitro tissue culture. Random amplified polymorphic DNA RAPD and coupled restriction enzyme digestion-random amplification CRED-RA were utilized to explain the DNA damage grade and variations in DNA methylation patterns, respectively. The outcomes indicate that drought stress gives rise to a rise in RAPD profile variations as DNA damage and a decrease in genomic template stability GTS rate and DNA methylation changes. According to the RAPD data, the greatest GTS value was computed at 56.9 5 PEG 6000, and the lowest GTS value was 41.2 15 PEG 6000, demonstrating the adverse effects of PEG 6000. However, DNA damage can be reduced by treatment with sodium selenate 2, 4, and 6 µM of Na2SeO4 together with PEG 5, 10, and 15 PEG 6000-induced water deficits. Moreover, according to CRED-RA analysis, PEG-induced DNA methylation rates were changed after treating different doses of Se. These data demonstrate that Se dose-dependently modulates both DNA damage and methylation alterations induced by drought in wheat.

Makale detayları

Effects of Selenium on DNA Methylation and Genomic Instability Induced by Drought Stress in Wheat (Triticum aestivum L.)

Yazarlar:

Zehra Şahin | Güleray Ağar | Esma Yiğider | Murat Aydın

Yayıncı:

Siirt University

Ana Başlıklar:

Bitki Biyoteknolojisi

Dergi:

Türkiye Tarımsal Araştırmalar Dergisi Vol.11 Issue 1, pp.26-37

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001315068

pISSN:

2148-2306

DNA damage

DNA methylation

drought stress

polyethylene glycol

seleniumDNA damage

DNA methylation

Drought stress

Selenium

polyethylene glycol

Sayı

Makale detayları

Yazarlar: Zehra Şahin | Güleray Ağar | Esma Yiğider | Murat Aydın

Yayıncı: Siirt University

Ana Başlıklar: Bitki Biyoteknolojisi

Dergi: Türkiye Tarımsal Araştırmalar Dergisi Vol.11 Issue 1, pp.26-37

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001315068

pISSN: 2148-2306

Sayı

Yazarlar:

Zehra Şahin | Güleray Ağar | Esma Yiğider | Murat Aydın

Yayıncı:

Siirt University

Ana Başlıklar:

Bitki Biyoteknolojisi

Dergi:

Türkiye Tarımsal Araştırmalar Dergisi Vol.11 Issue 1, pp.26-37

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001315068

pISSN:

2148-2306