Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol Induced-Kindling Rats

Savaş USTUNOVA; İsmail MERAL; Abdurrahim KOÇYİĞİT; Aysu KILIC; Oyku ZEYBEK; Mehmet ÜYÜKLÜ; Sedat MEYDAN

Makale detayları

Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol Induced-Kindling Rats

Yazarlar:

Savaş USTUNOVA | İsmail MERAL | Abdurrahim KOÇYİĞİT | Aysu KILIC | Oyku ZEYBEK | Mehmet ÜYÜKLÜ | Sedat MEYDAN

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2020 Vol.79 Issue 2, pp.124-131

Belge Tipi:

Makale

Yayın Tarihi:

12/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000424383

eISSN:

2618-6144

pISSN:

2602-2575

Özet:

 

Objective: This study was designed for investigating the effectiveness of the Nigella sativa (N. sativa) extract on apoptosis in cerebrum and hippocampus and also plasma oxidative stress, in pentylenetetrazol (PTZ)-induced kindling rats. Materials and Methods: The kindling model was induced by subconvulsive doses (35 mg/kg i.p.) of intraperitoneal PTZ injections in N. sativa treated and non-treated PTZ groups (PTZ and PTZ + NS). The PTZ + NS group were also treated with an extract of N. sativa (10 mg/kg) 2 h before each PTZ injection. The total oxidant and antioxidant status, oxidative stress index, paraoxonase, arylesterase, catalase (CAT) and total thiol levels were analyzed in plasma. Brain derived neurotrophic factor (BDNF), cyclin-B1 and B-cell lymphoma 2 (Bcl-2) expressions were investigated in the cerebrum and hippocampus.Results: PTZ decreased the oxidative stress by increasing the activities of CAT, arylesterase, and paraoxonase. N. sativa decreased activities of arylesterase, paraoxonase, while increasing the CAT. It also brought the decreased BDNF and Bcl-2 expression levels to their normal levels in the cerebrum but not in the hippocampus.Conclusion: N. sativa treatment improved the PTZ induced-impairments in BDNF and Bcl-2 expressions, resulting in a neuronal apoptosis in the cerebrum, without affecting blood oxidative stress.

Makale detayları

Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol Induced-Kindling Rats

Yazarlar: Savaş USTUNOVA | İsmail MERAL | Abdurrahim KOÇYİĞİT | Aysu KILIC | Oyku ZEYBEK | Mehmet ÜYÜKLÜ | Sedat MEYDAN

Yayıncı: Istanbul University

Dergi: European Journal of Biology 2020 Vol.79 Issue 2, pp.124-131

Belge Tipi: Makale

Yayın Tarihi: 12/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000424383

eISSN: 2618-6144

pISSN: 2602-2575

Sayı

Yazarlar:

Savaş USTUNOVA | İsmail MERAL | Abdurrahim KOÇYİĞİT | Aysu KILIC | Oyku ZEYBEK | Mehmet ÜYÜKLÜ | Sedat MEYDAN

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2020 Vol.79 Issue 2, pp.124-131

Belge Tipi:

Makale

Yayın Tarihi:

12/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000424383

eISSN:

2618-6144

pISSN:

2602-2575