Berberine Attenuates Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage Via Modulating AMPK/Rho Pathway

Ozge Altintas KADİRHAN; Meltem KUMAS; Ersin KARATAS; Hasan Serdar MUTLU; Abdurrahim KOCYİGİT; Talip ASİL

doi:https://doi.org/10.30621/jbachs.2020.1125

Makale detayları

Berberine Attenuates Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage Via Modulating AMPK/Rho Pathway

Yazarlar:

Ozge Altintas KADİRHAN | Meltem KUMAS | Ersin KARATAS | Hasan Serdar MUTLU | Abdurrahim KOCYİGİT | Talip ASİL

Yayıncı:

Dokuz Eylul University

Dergi:

Journal of Basic and Clinical Health Sciences 2020 Vol.4 Issue 3, pp.310-317

Belge Tipi:

Makale

Yayın Tarihi:

8/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000493440

Sıklık:

Üç Yıllık

eISSN:

2564-7288

DOI:

https://doi.org/10.30621/jbachs.2020.1125

Özet:

 

Purpose: Our goal is to clarify the effectiveness of berberine  BBR  on cerebral vasospasm induced by subarachnoid hemorrhage.  Methods: Thirty male Sprague-Dawley rats  350–400 g  were randomly allocated to five groups  sham group, SAH, BBR, SAH+BBR1 or  SAH+BBR2. Experimental SAH model was induced by applying autologous blood into the cisterna magna at interval of 48 hours. To evaluate  early and late effects of BBR, we allocated BBR treated groups as SAH+BBR1 and SAH+BBR2  respectively, received BBR at a dose of 20 mg/kg  15 minutes and 6 hours after first SAH induction . Rats were sacrificed on 72-hour after the study onset. Cross-sections of basilar artery was  investigated by histologically. Total antioxidant status  TAS  and total oxidant status  TOS  of brain tissue were studied by spectrophotometric  assay. Oxidative stress index  OSI  was calculated. NAPPH Oxidase 4  NOX4  enzyme levels were measured by ELISA method. Endothelial nitric  oxide synthase  e-NOS , phosphorylated e-NOS  pe-NOS , AMP-activated protein kinase  AMPK , phosphorylated AMPK  pAMPK , Rho kinase  and cingulin protein expressions were detected by Western blot analysis.  Results: SAH+BBR1 and SAH+BBR2 groups significantly demonstrated lower OSI values, increased basilar artery cross-sectional luminal area  in comparison with the SAH group. Increased Phosho-eNOS, eNOS, P-AMPK levels and Cingulin expression, decreased Nox4 and Rho-kinase  levels were shown in BRB treated SAH groups relative to the SAH group.  Conclusion: Berberine might be a neuroprotective agent to improve impaired cerebrovascular spasm.

Makale detayları

Berberine Attenuates Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage Via Modulating AMPK/Rho Pathway

Yazarlar: Ozge Altintas KADİRHAN | Meltem KUMAS | Ersin KARATAS | Hasan Serdar MUTLU | Abdurrahim KOCYİGİT | Talip ASİL

Yayıncı: Dokuz Eylul University

Dergi: Journal of Basic and Clinical Health Sciences 2020 Vol.4 Issue 3, pp.310-317

Belge Tipi: Makale

Yayın Tarihi: 8/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000493440

Sıklık: Üç Yıllık

eISSN: 2564-7288

DOI: https://doi.org/10.30621/jbachs.2020.1125

Sayı

Yazarlar:

Ozge Altintas KADİRHAN | Meltem KUMAS | Ersin KARATAS | Hasan Serdar MUTLU | Abdurrahim KOCYİGİT | Talip ASİL

Yayıncı:

Dokuz Eylul University

Dergi:

Journal of Basic and Clinical Health Sciences 2020 Vol.4 Issue 3, pp.310-317

Belge Tipi:

Makale

Yayın Tarihi:

8/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000493440

Sıklık:

Üç Yıllık

eISSN:

2564-7288

DOI:

https://doi.org/10.30621/jbachs.2020.1125