Host-Specific Toxin Production by the Tomato Target Leaf Spot Pathogen Corynespora cassiicola

Şener KURT*

Makale detayları

Host-Specific Toxin Production by the Tomato Target Leaf Spot Pathogen Corynespora cassiicola

Yazarlar:

Şener KURT*

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2004 Vol.28 Issue 6, pp.389-395

Belge Tipi:

Makale

Yayın Tarihi:

5/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000809110

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

The culture filtrate (CF) of Corynespora cassiicola, the causal agent of target leaf spot of tomato, produced visible small, pinpoint, water-soaked lesions on the lower surface of wounded, detached leaves after incubation for 48 h. The extraction, isolation and purification of C. cassiicola tomato (CCT) toxin was achieved by anion exchange and gel filtration chromatography. Treatments of the CF with Diaion HP-20 resin and Cosmosil C18-OPN gel were found to be highly effective. The toxin purified partially by cosmosil gel filtration was highly active against the tomato cultivar Momotaro used in the bioassay test, and the fraction eluted with 50% methanol showed toxicity only on the tomato plant. However, in the bioassay of the fractions after Sephadex LH-20 gel filtration no toxicity could be detected. Moreover, methanol fractions (40% and 60%) of the culture filtrate extracted by Sep-Pak cartridge were subjected to high performance liquid chromatography systems. The bioactive fraction (40% methanol) selected for analysis was eluted as double peaks. The results showed that C. cassiicola produced a host-specific toxin during colonization of tomato leaves and disease incidence.

 

The culture filtrate (CF) of Corynespora cassiicola, the causal agent of target leaf spot of tomato, produced visible small, pinpoint, water-soaked lesions on the lower surface of wounded, detached leaves after incubation for 48 h. The extraction, isolation and purification of C. cassiicola tomato (CCT) toxin was achieved by anion exchange and gel filtration chromatography. Treatments of the CF with Diaion HP-20 resin and Cosmosil C18-OPN gel were found to be highly effective. The toxin purified partially by cosmosil gel filtration was highly active against the tomato cultivar Momotaro used in the bioassay test, and the fraction eluted with 50% methanol showed toxicity only on the tomato plant. However, in the bioassay of the fractions after Sephadex LH-20 gel filtration no toxicity could be detected. Moreover, methanol fractions (40% and 60%) of the culture filtrate extracted by Sep-Pak cartridge were subjected to high performance liquid chromatography systems. The bioactive fraction (40% methanol) selected for analysis was eluted as double peaks. The results showed that C. cassiicola produced a host-specific toxin during colonization of tomato leaves and disease incidence.

Makale detayları

Host-Specific Toxin Production by the Tomato Target Leaf Spot Pathogen Corynespora cassiicola

Yazarlar:

Şener KURT*

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2004 Vol.28 Issue 6, pp.389-395

Belge Tipi:

Makale

Yayın Tarihi:

5/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000809110

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Corynespora cassiicola

host-specific toxin (HST)

target leaf spot

tomato

Sayı

Makale detayları

Host-Specific Toxin Production by the Tomato Target Leaf Spot Pathogen Corynespora cassiicola

Yazarlar: Şener KURT*

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2004 Vol.28 Issue 6, pp.389-395

Belge Tipi: Makale

Yayın Tarihi: 5/2004

Belge Dili: Türkçe

Document ID (Org No.): 2000809110

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Corynespora cassiicola

host-specific toxin (HST)

target leaf spot

tomato

Sayı

Yazarlar:

Şener KURT*

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2004 Vol.28 Issue 6, pp.389-395

Belge Tipi:

Makale

Yayın Tarihi:

5/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000809110

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X