Development of Simplex-PCR assays for Accurate Identification of Nine Staphylococcal Species at Genus and Species Levels

Bibek Ranjan SHOME; Krithiga NATESAN; Susweta Das MİTRA; Nimita VENUGOPAL; Bhuvana MANİ; Feroze GANAİE; Rajeswari SHOME; Habibur RAHMAN

doi:https://doi.org/10.5799/jmid.458462

Makale detayları

Development of Simplex-PCR assays for Accurate Identification of Nine Staphylococcal Species at Genus and Species Levels

Yazarlar:

Bibek Ranjan SHOME | Krithiga NATESAN | Susweta Das MİTRA | Nimita VENUGOPAL | Bhuvana MANİ | Feroze GANAİE | Rajeswari SHOME | Habibur RAHMAN

Yayıncı:

Sağlık Araştırmaları Derneği

Dergi:

Journal of Microbiology and Infectious Diseases 2018 Vol.08 Issue 03, pp.120-127

Belge Tipi:

Makale

Yayın Tarihi:

9/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000487392

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/jmid.458462

Özet:

 


Objective: Species identification of Staphylococcus is the prerequisite for precise assessment of
microbial dynamics including their transmission and pathogenic significance in
the dairy herd environment. The present study aimed to develop simplex PCR
assays for rapid and specific identification of nine different Staphylococcus species. 

Methods: Specific
primers targeting sodA gene for S. aureus, S. chromogenes, S. hominis,
S. haemolyticus, S. hyicus; gap gene for S. sciuri, S. auricularis, S. simulans;
and rdr gene for S. epidermidis were designed. The PCR assays were evaluated against
28 ATCC reference strains and 209 composite milk samples. Partial 16S rRNA sequencing was performed to
reconfirm the results. 

Results: The
PCR assays allowed species level identification of 348 staphylococcal field
isolates recovered from 209 milk samples. The identification pattern was S. aureus (n=101), S. chromogenes (n=89), S.
epidermidis (n=57), S. sciuri
(n=43), S. haemolyticus (n=34), S. hyicus (n=13), S. hominis (n=5), S.
auricularis (n=3) and S. simulans (n=3). The PCR based species
identification was in 100% concordance with the partial 16S rRNA gene sequencing approach.

Conclusion: The simplex PCR assays can be used as a precise tool
for routine identification of Staphylococcus
species from bovine milk as Staphylococcus
species including coagulase-negative staphylococci is recognized a major cause
of bovine mastitis in different parts of the world including India. J Microbiol Infect Dis 2018; 8(3):120-127

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Makale detayları

Development of Simplex-PCR assays for Accurate Identification of Nine Staphylococcal Species at Genus and Species Levels

Yazarlar: Bibek Ranjan SHOME | Krithiga NATESAN | Susweta Das MİTRA | Nimita VENUGOPAL | Bhuvana MANİ | Feroze GANAİE | Rajeswari SHOME | Habibur RAHMAN

Yayıncı: Sağlık Araştırmaları Derneği

Dergi: Journal of Microbiology and Infectious Diseases 2018 Vol.08 Issue 03, pp.120-127

Belge Tipi: Makale

Yayın Tarihi: 9/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000487392

Sıklık: Üç Aylık

eISSN: 2146-9369

pISSN: 2146-3158

DOI: https://doi.org/10.5799/jmid.458462

Sayı

Yazarlar:

Bibek Ranjan SHOME | Krithiga NATESAN | Susweta Das MİTRA | Nimita VENUGOPAL | Bhuvana MANİ | Feroze GANAİE | Rajeswari SHOME | Habibur RAHMAN

Yayıncı:

Sağlık Araştırmaları Derneği

Dergi:

Journal of Microbiology and Infectious Diseases 2018 Vol.08 Issue 03, pp.120-127

Belge Tipi:

Makale

Yayın Tarihi:

9/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000487392

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/jmid.458462