A Study on the Factors Affecting the Growth of Staphylococus aureus Strains and Enterotoxin Production in Cream Pastries

Mustafa ALİŞARLI; Emrullah SAĞUN; Süleyman ALEMDAR; Levent AKKAYA

Makale detayları

A Study on the Factors Affecting the Growth of Staphylococus aureus Strains and Enterotoxin Production in Cream Pastries

Yazarlar:

Mustafa ALİŞARLI | Emrullah SAĞUN | Süleyman ALEMDAR | Levent AKKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 3, pp.535-542

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770728

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

In this study, the growth and enterotoxin production abilities of enterotoxigenic S. aureus strains in cream pastries were investigated. The cream was inoculated with enterotoxigenic S. aureus strains, which produce A (SEA 10652 FDA 196E), B (SEB 10654 FDA 243), C (SEC 10655 137) and D (SED 10656 494) type toxins as mixtures at 103, 104 and 105 cfu/g levels. Cream pastry samples produced with these cream mixtures were stored at 4°C, 10°C, 18°C, room temperature (23-26°C) and 30°C for 48 hours. The samples taken after 2, 6, 12, 24 and 48 hours during this experiment were analysed microbiologically, physico-chemically and serologically.  None of the strains produced enterotoxin in cream pastry samples contaminated at 103 cfu/g and stored at 4°C, 10°C and 18°C. Enerotoxin A, B, C and D were determined at 30°C after 12, 18, 48 and 24 hours, respectively. At room temperature enterotoxin C was not detected but enterotoxin A and D were observed after 24 hours, while the observation time was 48 hours for enterotoxin B. In cream pastry samples contaminated at 104 cfu/g and stored at 18°C, only entrerotoxin A was detected after 48 hours. In the samples stored at room temperature, A, B, D and C were observed  after 12, 18, 24 and 48 hours, respectively. At 30°C, enterotoxin A, B, D and C were detected after 6, 12, 18 and 24 hours, respectively. In the other cream pastry samples contaminated at 105 cfu/g and stored at 18°C, entertoxin A was observed after 24 hours. At room temperature enterotoxin A and B were observed after 12 hours and enterotoxin C and D were observed after 18 hours. At 30°C, enterotoxin A and D were detected after 6 hours and enterotoxin B and D were detected after 12 hours.  Therefore, considering that especially S. aureus strains producing enterotoxin A can produce toxins at low temperatures, it is very important to store food products containing these types of strain in cold conditions and to take necessary precautions in order to prevent contamination with S. aureus strains which produces enterotoxin. Furthermore, necessary hygienic measures should be taken by considering S. aureus strains, especially those producing A type enterotoxin.

 

In this study, the growth and enterotoxin production abilities of enterotoxigenic S. aureus strains in cream pastries were investigated. The cream was inoculated with enterotoxigenic S. aureus strains, which produce A (SEA 10652 FDA 196E), B (SEB 10654 FDA 243), C (SEC 10655 137) and D (SED 10656 494) type toxins as mixtures at 103, 104 and 105 cfu/g levels. Cream pastry samples produced with these cream mixtures were stored at 4°C, 10°C, 18°C, room temperature (23-26°C) and 30°C for 48 hours. The samples taken after 2, 6, 12, 24 and 48 hours during this experiment were analysed microbiologically, physico-chemically and serologically.  None of the strains produced enterotoxin in cream pastry samples contaminated at 103 cfu/g and stored at 4°C, 10°C and 18°C. Enerotoxin A, B, C and D were determined at 30°C after 12, 18, 48 and 24 hours, respectively. At room temperature enterotoxin C was not detected but enterotoxin A and D were observed after 24 hours, while the observation time was 48 hours for enterotoxin B. In cream pastry samples contaminated at 104 cfu/g and stored at 18°C, only entrerotoxin A was detected after 48 hours. In the samples stored at room temperature, A, B, D and C were observed  after 12, 18, 24 and 48 hours, respectively. At 30°C, enterotoxin A, B, D and C were detected after 6, 12, 18 and 24 hours, respectively. In the other cream pastry samples contaminated at 105 cfu/g and stored at 18°C, entertoxin A was observed after 24 hours. At room temperature enterotoxin A and B were observed after 12 hours and enterotoxin C and D were observed after 18 hours. At 30°C, enterotoxin A and D were detected after 6 hours and enterotoxin B and D were detected after 12 hours.  Therefore, considering that especially S. aureus strains producing enterotoxin A can produce toxins at low temperatures, it is very important to store food products containing these types of strain in cold conditions and to take necessary precautions in order to prevent contamination with S. aureus strains which produces enterotoxin. Furthermore, necessary hygienic measures should be taken by considering S. aureus strains, especially those producing A type enterotoxin.

Makale detayları

A Study on the Factors Affecting the Growth of Staphylococus aureus Strains and Enterotoxin Production in Cream Pastries

Yazarlar:

Mustafa ALİŞARLI | Emrullah SAĞUN | Süleyman ALEMDAR | Levent AKKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 3, pp.535-542

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770728

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Cream pastry

S. aureus

Growth

Enterotoxin production

Sayı

Makale detayları

A Study on the Factors Affecting the Growth of Staphylococus aureus Strains and Enterotoxin Production in Cream Pastries

Yazarlar: Mustafa ALİŞARLI | Emrullah SAĞUN | Süleyman ALEMDAR | Levent AKKAYA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 3, pp.535-542

Belge Tipi: Makale

Yayın Tarihi: 2/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000770728

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Cream pastry

S. aureus

Growth

Enterotoxin production

Sayı

Yazarlar:

Mustafa ALİŞARLI | Emrullah SAĞUN | Süleyman ALEMDAR | Levent AKKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 3, pp.535-542

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770728

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128