In vitro Regeneration of Vanilla (Vanilla planifolia L.)

Raihana HALİM; Begüm AKYOL; Aynur GÜNER

Makale detayları

In vitro Regeneration of Vanilla (Vanilla planifolia L.)

Yazarlar:

Raihana HALİM | Begüm AKYOL | Aynur GÜNER

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2017 Vol.11 Issue 1, pp.5-10

Belge Tipi:

Makale

Yayın Tarihi:

4/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000831107

Sıklık:

Üç Yıllık

eISSN:

2146-0108

Özet:

 


Vanilla is a high demanding product sold at high prices in world markets. Natural vanilla has high economic value and is theworld’s second most expensive spices after saffron. Despite its economic value, it has been one of the least studied species of vanilla plants among other orchid species. Vanilla’s synthetic form (vanillin) can be obtained from various chemical methods by using starting materials (coniferin, guaiacol, eugenol and lignin), but the vanilla flavor can naturally be obtained only from vanilla fruit. Micropropagation is an alternative technique for economically important plant production more effectively used with minimum starting material. For this reason, in the study, regeneration of the vanilla plant in vitro were carried out. Seed, young leaves, node and  maerial parts of V. planifolia L. were used as an initial material. The highest callus regeneration (80%) from node explants was obtained in VK4 (MS+1 mg/L 2,4-D + 1 mg/L BAP) nutrient medium, while 100% direct shoot regeneration was provided in VK3 (MS+1 mg/L 2,4-D + 0,5 mg/L BAP) and VK6 (MS+1 mg/L BAP + 0,5 mg/L NAA) nutrient media. In addition, 50% rooting percentage was obtained in VK6 medium. Indirect shoot regeneration ratio was determined as 33,3% in VK2 (MS + 1 mg / L 2,4-D + 0,5 mg / L KIN) and 71,4% of indirect embryo formation was detected in VK4 medium. All embryos stayed in globules stages

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Makale detayları

In vitro Regeneration of Vanilla (Vanilla planifolia L.)

Yazarlar: Raihana HALİM | Begüm AKYOL | Aynur GÜNER

Yayıncı: Nobel Bilim ve Araştırma Merkezi Limited

Dergi: Journal of Applied Biological Sciences 2017 Vol.11 Issue 1, pp.5-10

Belge Tipi: Makale

Yayın Tarihi: 4/2017

Belge Dili: İngilizce

Document ID (Org No.): 2000831107

Sıklık: Üç Yıllık

eISSN: 2146-0108

Sayı

Yazarlar:

Raihana HALİM | Begüm AKYOL | Aynur GÜNER

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2017 Vol.11 Issue 1, pp.5-10

Belge Tipi:

Makale

Yayın Tarihi:

4/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000831107

Sıklık:

Üç Yıllık

eISSN:

2146-0108