Limitations of Cross-Talk Between Osteosarcoma and Bone Marrow-Derived Mesenchymal Stem Cells

Yasemin BASBİNAR; Tugba UYSAL; Caner KARACA; Ezgi DASKİN; Hanife Ecenur MECO; Ahu PAKDEMİRLİ; Turkan YİGİTBASİ; Hulya ELLİDOKUZ

doi:https://doi.org/10.30621/jbachs.2019.611

Makale detayları

Limitations of Cross-Talk Between Osteosarcoma and Bone Marrow-Derived Mesenchymal Stem Cells

Yazarlar:

Yasemin BASBİNAR | Tugba UYSAL | Caner KARACA | Ezgi DASKİN | Hanife Ecenur MECO | Ahu PAKDEMİRLİ | Turkan YİGİTBASİ | Hulya ELLİDOKUZ

Yayıncı:

Dokuz Eylul University

Dergi:

Journal of Basic and Clinical Health Sciences 2019 Vol.3 Issue 2, pp.83-88

Belge Tipi:

Makale

Yayın Tarihi:

5/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000493635

Sıklık:

Üç Yıllık

eISSN:

2564-7288

DOI:

https://doi.org/10.30621/jbachs.2019.611

Özet:

 

Objectives: Metastasis is a multi-step process which leads the tumor cells to escape from primer tumor region due to their need to gain malign phenotypes. While the effect of bone marrow-derived mesenchymal stem cells upon metastasis is not certain, some studies point out bone marrow-derived mesenchymal stem cells  BM-MSCs  to have this ability due to cell-cell interaction, released cytokines, and organization with the extracellular matrix in the micro-environment. Cross-talk via soluble factors also shifts the metastatic character.Patients and Methods: In this study, the effects of mesenchymal stem cells on tumor behavior by creating different microenvironments in 3-dimensional  3D  in vitro cancer model is analyzed. The BM-MSCs and osteosarcoma cells were co-cultured via hanging-drop modeled 3D structure in normoxic and hypoxic conditions, and the cross-talk was modeled to measure their chemoattractant effects. The invasion and migration rates were measured with xCELLigence DP real-time cell analysis system. Mann Whitney U Test was used to compare independent samples. All P-values.

Makale detayları

Limitations of Cross-Talk Between Osteosarcoma and Bone Marrow-Derived Mesenchymal Stem Cells

Yazarlar: Yasemin BASBİNAR | Tugba UYSAL | Caner KARACA | Ezgi DASKİN | Hanife Ecenur MECO | Ahu PAKDEMİRLİ | Turkan YİGİTBASİ | Hulya ELLİDOKUZ

Yayıncı: Dokuz Eylul University

Dergi: Journal of Basic and Clinical Health Sciences 2019 Vol.3 Issue 2, pp.83-88

Belge Tipi: Makale

Yayın Tarihi: 5/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000493635

Sıklık: Üç Yıllık

eISSN: 2564-7288

DOI: https://doi.org/10.30621/jbachs.2019.611

Sayı

Yazarlar:

Yasemin BASBİNAR | Tugba UYSAL | Caner KARACA | Ezgi DASKİN | Hanife Ecenur MECO | Ahu PAKDEMİRLİ | Turkan YİGİTBASİ | Hulya ELLİDOKUZ

Yayıncı:

Dokuz Eylul University

Dergi:

Journal of Basic and Clinical Health Sciences 2019 Vol.3 Issue 2, pp.83-88

Belge Tipi:

Makale

Yayın Tarihi:

5/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000493635

Sıklık:

Üç Yıllık

eISSN:

2564-7288

DOI:

https://doi.org/10.30621/jbachs.2019.611