Availibility of use of Total Extracellular Proteins in  SDS-PAGE for Characterization of Gram-Positive Cocci

Sefa C. SAÇILIK

Makale detayları

Availibility of use of Total Extracellular Proteins in SDS-PAGE for Characterization of Gram-Positive Cocci

Yazarlar:

Sefa C. SAÇILIK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.817-824

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813955

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

There are no reports in the literature dealing with gram-positive cocci typing by sodium  dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of extracellular proteins. In this study,  Staphylococcus aureus, Staphylococcus warneri, Staphylococcus chromogenes, Micrococcus  lysodeikticus and Micrococcus ssp. of the Micrococcaceae family, and Pediococcus halophilus,  Streptococcus ssp. and Enterococcus faecalis of the Streptococcaceae family were characterized by  SDS-PAGE of whole cell proteins (WCPs) and total extracellular proteins (TEPs). Comparison of SDS-PAGE  of WCP profiles with banding patterns produced by that of TEPs provided additional criteria and  characteristic banding patterns for the study of the characterization of gram-positive cocci. This  method was found to be easy to use and interpret and provided a rapid, reproducible and  discriminative method for typing gram-positive cocci.

 

There are no reports in the literature dealing with gram-positive cocci typing by sodium  dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of extracellular proteins. In this study,  Staphylococcus aureus, Staphylococcus warneri, Staphylococcus chromogenes, Micrococcus  lysodeikticus and Micrococcus ssp. of the Micrococcaceae family, and Pediococcus halophilus,  Streptococcus ssp. and Enterococcus faecalis of the Streptococcaceae family were characterized by  SDS-PAGE of whole cell proteins (WCPs) and total extracellular proteins (TEPs). Comparison of SDS-PAGE  of WCP profiles with banding patterns produced by that of TEPs provided additional criteria and  characteristic banding patterns for the study of the characterization of gram-positive cocci. This  method was found to be easy to use and interpret and provided a rapid, reproducible and  discriminative method for typing gram-positive cocci.

Makale detayları

Availibility of use of Total Extracellular Proteins in SDS-PAGE for Characterization of Gram-Positive Cocci

Yazarlar:

Sefa C. SAÇILIK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.817-824

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813955

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

SDS-PAGE

gram-positive cocci

characterization.

Sayı

Makale detayları

Availibility of use of Total Extracellular Proteins in SDS-PAGE for Characterization of Gram-Positive Cocci

Yazarlar: Sefa C. SAÇILIK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.817-824

Belge Tipi: Makale

Yayın Tarihi: 3/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000813955

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

SDS-PAGE

gram-positive cocci

characterization.

Sayı

Yazarlar:

Sefa C. SAÇILIK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.817-824

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813955

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152