Overexpression of the IbOr gene from sweet potato (Ipomea batatas `Hoang Long') in maize increases total carotenoid and ß-carotene contents

Thi Luong TRAN; Thi Huong HO; Duc Thanh NGUYEN

Makale detayları

Overexpression of the IbOr gene from sweet potato (Ipomea batatas `Hoang Long') in maize increases total carotenoid and ß-carotene contents

Yazarlar:

Thi Luong TRAN | Thi Huong HO | Duc Thanh NGUYEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 6, pp.1003-1010

Belge Tipi:

Makale

Yayın Tarihi:

11/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811318

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Nutritional quality of most maize varieties is very low due to the lack of lysine and tryptophan and extremely low provitamin A carotenoids including ß-carotene, Α-carotene, and ß-cryptoxanthin. In this study, we report the successful overexpression of the IbOr gene in H145 and H95 inbred maize lines under the control of maize seed-specific promoter globulin 1 (Glo1) for the purpose of improving ß-carotene in maize. The results showed that the total carotenoid and ß-carotene content of all analyzed transgenic maize plants were significantly higher than those of wild-type lines. For H145-IbOr transgenic maize, in the best line (H145-IbOr.10), the total carotenoid and ß-carotene contents were increased up to 10.36- and 15.11-fold, respectively, compared to the wild type (H145-WT). In the case of H95-IbOr transgenic plants, 5.58-fold increase in total carotenoid and 7.63-fold increase in ß-carotene were achieved in the H95-IbOr.6 line compared to nontransgenic plants (H95-WT). In all the transgenic plants derived from the wild-type maize line with less carotenoid content (H145-WT), the content of both total carotenoid and ß-carotene was higher than in transgenic plants derived from the wild-type maize line having more carotenoid content (H95-WT). Our research is the first in successful overexpression of IbOr gene in maize.

Makale detayları

Overexpression of the IbOr gene from sweet potato (Ipomea batatas `Hoang Long') in maize increases total carotenoid and ß-carotene contents

Yazarlar: Thi Luong TRAN | Thi Huong HO | Duc Thanh NGUYEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2017 Vol.41 Issue 6, pp.1003-1010

Belge Tipi: Makale

Yayın Tarihi: 11/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000811318

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Thi Luong TRAN | Thi Huong HO | Duc Thanh NGUYEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 6, pp.1003-1010

Belge Tipi:

Makale

Yayın Tarihi:

11/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811318

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152