Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image

Gerd H. WOEHRLE; James E. HUTCHISON; Saim ÖZKAR; Richard G. FINKE

Makale detayları

Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image

Yazarlar:

Gerd H. WOEHRLE | James E. HUTCHISON | Saim ÖZKAR | Richard G. FINKE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2006 Vol.30 Issue 1, pp.1-13

Belge Tipi:

Makale

Yayın Tarihi:

12/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000728943

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

The need to easily and quickly count larger numbers of  nanoparticles, in order to obtain statistically useful size and  size-distribution data, is addressed via the use of a readily  available, free, public-domain program for particle counting,  NIH-Image (and 2 others derived from it, Scion Image and Image J),  collectively referred to herein as Image.  The best protocols that we  have found useful for the use of Image are reported; both appropriate  as well as problematic applications of Image are then illustrated with  a series of TEM images of Ir(0), Pd(0) and Au(0) nanoclusters.   Methods to detect and image nanoclusters with sub-1-nm core diameters  are reported and illustrated in the literature with Au nanoclusters,  an important problem since the literature indicates that subnanometer  Au nanoclusters are often present, but undetected.  A list of  suggestions and caveats for the appropriate use of Image is also  provided, since this contribution is directed at first-time users who  are not presently using nanoparticle imaging software.  The reader is  also reminded of several, well-known caveats for the use of TEM in  obtaining size data for nanostructures.  Overall, Image is a free,  public-domain program that is useful for the rapid counting of large  numbers of particles.

 

The need to easily and quickly count larger numbers of  nanoparticles, in order to obtain statistically useful size and  size-distribution data, is addressed via the use of a readily  available, free, public-domain program for particle counting,  NIH-Image (and 2 others derived from it, Scion Image and Image J),  collectively referred to herein as Image.  The best protocols that we  have found useful for the use of Image are reported; both appropriate  as well as problematic applications of Image are then illustrated with  a series of TEM images of Ir(0), Pd(0) and Au(0) nanoclusters.   Methods to detect and image nanoclusters with sub-1-nm core diameters  are reported and illustrated in the literature with Au nanoclusters,  an important problem since the literature indicates that subnanometer  Au nanoclusters are often present, but undetected.  A list of  suggestions and caveats for the appropriate use of Image is also  provided, since this contribution is directed at first-time users who  are not presently using nanoparticle imaging software.  The reader is  also reminded of several, well-known caveats for the use of TEM in  obtaining size data for nanostructures.  Overall, Image is a free,  public-domain program that is useful for the rapid counting of large  numbers of particles.

Makale detayları

Analysis of Nanoparticle Transmission Electron Microscopy Data Using a Public- Domain Image-Processing Program, Image

Yazarlar: Gerd H. WOEHRLE | James E. HUTCHISON | Saim ÖZKAR | Richard G. FINKE

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2006 Vol.30 Issue 1, pp.1-13

Belge Tipi: Makale

Yayın Tarihi: 12/2005

Belge Dili: Türkçe

Document ID (Org No.): 2000728943

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Gerd H. WOEHRLE | James E. HUTCHISON | Saim ÖZKAR | Richard G. FINKE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2006 Vol.30 Issue 1, pp.1-13

Belge Tipi:

Makale

Yayın Tarihi:

12/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000728943

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527