Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections

N.A.

Makale detayları

Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections

Yazarlar:

N.A.

Yayıncı:

Kafkas University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Kafkas Tıp Bilimleri Dergisi Vol.13 Issue 2, pp.197-205

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294180

pISSN:

2146-2631

Anahtar kelimeler:

Özet:

 Aim: Acinetobacter baumannii is an important pathogen causing concern worldwide with increasing antibiotic resistance. We aimed to provide epidemiological data and molecular differences between multidrug resistant A.baumannii isolates obtained from 7 different centers in Türkiye. Material and Method: One hundred sixty-six multidrug-resistant A.baumannii strains were included in the study. All the isolates were tested for antibiotic susceptibility according to EUCAST criteria. To molecularly confirm the carbapenem-resistant A.baumannii isolates, the specific blaOXA-51-like gene region was detected by polymerase chain reaction PCR method. A possible phylogenetic relationship between multi-drug resistant isolates was investigated using Pulsed-field Gel Electrophoresis method. Results: One hundred sixty-six carbapenem-resistant A.baumannii strains were evaluated by the ApaI-PFGE Pulsed-field gel electrophoresis PFGE method using the CHEF-DRII electrophoresis system. A total of 51 clusters were grouped into 142 pulsotypes. Twenty clusters had single members. Thirty-one clusters had multiple members and were identified with the capital letters of the alphabet such as A, B, , and Z26. It was observed that 40 PFGE profiles showed 100 related. Conclusion: More intense contamination is in question in the provinces with many patients. In four of the seven different centers, more innocent results were obtained in terms of the number of samples, antibiotic resistance and clonal relationship when compared to the other three centers. However, this may be caused by the low population zone and inadequate health services. Regional or multicenter studies are minimal. Thus, further studies involving more centers and larger populations are needed. Consequently, the present study will shed light on future studies and create thriving literature on border regions.

 Aim: Acinetobacter baumannii is an important pathogen causing concern worldwide with increasing antibiotic resistance. We aimed to provide epidemiological data and molecular differences between multidrug resistant A.baumannii isolates obtained from 7 different centers in Türkiye. Material and Method: One hundred sixty-six multidrug-resistant A.baumannii strains were included in the study. All the isolates were tested for antibiotic susceptibility according to EUCAST criteria. To molecularly confirm the carbapenem-resistant A.baumannii isolates, the specific blaOXA-51-like gene region was detected by polymerase chain reaction PCR method. A possible phylogenetic relationship between multi-drug resistant isolates was investigated using Pulsed-field Gel Electrophoresis method. Results: One hundred sixty-six carbapenem-resistant A.baumannii strains were evaluated by the ApaI-PFGE Pulsed-field gel electrophoresis PFGE method using the CHEF-DRII electrophoresis system. A total of 51 clusters were grouped into 142 pulsotypes. Twenty clusters had single members. Thirty-one clusters had multiple members and were identified with the capital letters of the alphabet such as A, B, , and Z26. It was observed that 40 PFGE profiles showed 100 related. Conclusion: More intense contamination is in question in the provinces with many patients. In four of the seven different centers, more innocent results were obtained in terms of the number of samples, antibiotic resistance and clonal relationship when compared to the other three centers. However, this may be caused by the low population zone and inadequate health services. Regional or multicenter studies are minimal. Thus, further studies involving more centers and larger populations are needed. Consequently, the present study will shed light on future studies and create thriving literature on border regions.

 Aim: Acinetobacter baumannii is an important pathogen causing concern worldwide with increasing antibiotic resistance. We aimed to provide epidemiological data and molecular differences between multidrug resistant A.baumannii isolates obtained from 7 different centers in Türkiye. Material and Method: One hundred sixty-six multidrug-resistant A.baumannii strains were included in the study. All the isolates were tested for antibiotic susceptibility according to EUCAST criteria. To molecularly confirm the carbapenem-resistant A.baumannii isolates, the specific blaOXA-51-like gene region was detected by polymerase chain reaction PCR method. A possible phylogenetic relationship between multi-drug resistant isolates was investigated using Pulsed-field Gel Electrophoresis method. Results: One hundred sixty-six carbapenem-resistant A.baumannii strains were evaluated by the ApaI-PFGE Pulsed-field gel electrophoresis PFGE method using the CHEF-DRII electrophoresis system. A total of 51 clusters were grouped into 142 pulsotypes. Twenty clusters had single members. Thirty-one clusters had multiple members and were identified with the capital letters of the alphabet such as A, B, , and Z26. It was observed that 40 PFGE profiles showed 100 related. Conclusion: More intense contamination is in question in the provinces with many patients. In four of the seven different centers, more innocent results were obtained in terms of the number of samples, antibiotic resistance and clonal relationship when compared to the other three centers. However, this may be caused by the low population zone and inadequate health services. Regional or multicenter studies are minimal. Thus, further studies involving more centers and larger populations are needed. Consequently, the present study will shed light on future studies and create thriving literature on border regions.

 Aim: Acinetobacter baumannii is an important pathogen causing concern worldwide with increasing antibiotic resistance. We aimed to provide epidemiological data and molecular differences between multidrug resistant A.baumannii isolates obtained from 7 different centers in Türkiye. Material and Method: One hundred sixty-six multidrug-resistant A.baumannii strains were included in the study. All the isolates were tested for antibiotic susceptibility according to EUCAST criteria. To molecularly confirm the carbapenem-resistant A.baumannii isolates, the specific blaOXA-51-like gene region was detected by polymerase chain reaction PCR method. A possible phylogenetic relationship between multi-drug resistant isolates was investigated using Pulsed-field Gel Electrophoresis method. Results: One hundred sixty-six carbapenem-resistant A.baumannii strains were evaluated by the ApaI-PFGE Pulsed-field gel electrophoresis PFGE method using the CHEF-DRII electrophoresis system. A total of 51 clusters were grouped into 142 pulsotypes. Twenty clusters had single members. Thirty-one clusters had multiple members and were identified with the capital letters of the alphabet such as A, B, , and Z26. It was observed that 40 PFGE profiles showed 100 related. Conclusion: More intense contamination is in question in the provinces with many patients. In four of the seven different centers, more innocent results were obtained in terms of the number of samples, antibiotic resistance and clonal relationship when compared to the other three centers. However, this may be caused by the low population zone and inadequate health services. Regional or multicenter studies are minimal. Thus, further studies involving more centers and larger populations are needed. Consequently, the present study will shed light on future studies and create thriving literature on border regions.

Makale detayları

Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections

Yazarlar:

N.A.

Yayıncı:

Kafkas University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Kafkas Tıp Bilimleri Dergisi Vol.13 Issue 2, pp.197-205

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294180

pISSN:

2146-2631

A.baumannii

blaOXA-51-like gene

carbapenem-resistant

pulsed-field gel electrophoresis; PCR

Sayı

Makale detayları

Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections

Yazarlar: N.A.

Yayıncı: Kafkas University

Ana Başlıklar: Klinik Mikrobiyoloji

Dergi: Kafkas Tıp Bilimleri Dergisi Vol.13 Issue 2, pp.197-205

Belge Tipi: Makale

Yayın Tarihi: 8/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001294180

pISSN: 2146-2631

A.baumannii

blaOXA-51-like gene

carbapenem-resistant

pulsed-field gel electrophoresis; PCR

Sayı

Yazarlar:

N.A.

Yayıncı:

Kafkas University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Kafkas Tıp Bilimleri Dergisi Vol.13 Issue 2, pp.197-205

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294180

pISSN:

2146-2631