Influence of Dental Alloys and an All-Ceramic Material on Cell Viability and Interleukin-1beta Release in a Three-Dimensional Cell Culture Model

Jülide ÖZEN; Ali Uğur URAL; Mehmet DALKIZ; Bedri BEYDEMİR

Makale detayları

Influence of Dental Alloys and an All-Ceramic Material on Cell Viability and Interleukin-1beta Release in a Three-Dimensional Cell Culture Model

Yazarlar:

Jülide ÖZEN | Ali Uğur URAL | Mehmet DALKIZ | Bedri BEYDEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2005 Vol.35 Issue 4, pp.203-208

Belge Tipi:

Makale

Yayın Tarihi:

3/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000756347

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

The purpose of this study was to determine the influence of various types of dental casting alloys and ceramic upon cell viability and the synthesis of IL-1beta (b) in a three-dimensional cell culture system consisting of human gingival fibroblast, and to determine their effect in gingival inflammation. Au-Pt-In alloy (Pontostar), Ni-Cr-Mo alloy (Remanium-CS), a titanium alloy (Ti-6Al-4V), copper (Cu), and an all ceramic (In-Ceram) were used as test materials. The materials were exposed to a three dimensional cell culture in order to determine their effect on cell viability and IL-1 (b) secretion level. Cell viability was measured by MTT test after exposure for 24h and 48h. For IL-1 (b)  measurement, assay aliquots were taken from exposed media after 1h, 3h, 7h, 24h and 48h. Assays for IL-1 (b)  were carried out by ELISA. In-Ceram, Pontostar and Ti-6Al-4V alloy did not influence cell viability. Copper (52-64%), and Remanium CS (17-20%) were found to be cytotoxic, compared to control cultures. In-Ceram, Pontostar and Ti-6Al-4V had no significant influence on IL-1 (b)  secretion. Remanium-CS increased IL-1(b)  levels two fold compared to untreated control cultures. Our findings suggested that Ti-6Al-4V, In-Ceram and Pontostar did not cause elevated IL-1 (b)  release from cells at non-toxic levels. On the other hand, Remanium-CS showed moderate toxicity and caused increase in IL-1 (b)  levels.

 

The purpose of this study was to determine the influence of various types of dental casting alloys and ceramic upon cell viability and the synthesis of IL-1beta (b) in a three-dimensional cell culture system consisting of human gingival fibroblast, and to determine their effect in gingival inflammation. Au-Pt-In alloy (Pontostar), Ni-Cr-Mo alloy (Remanium-CS), a titanium alloy (Ti-6Al-4V), copper (Cu), and an all ceramic (In-Ceram) were used as test materials. The materials were exposed to a three dimensional cell culture in order to determine their effect on cell viability and IL-1 (b) secretion level. Cell viability was measured by MTT test after exposure for 24h and 48h. For IL-1 (b)  measurement, assay aliquots were taken from exposed media after 1h, 3h, 7h, 24h and 48h. Assays for IL-1 (b)  were carried out by ELISA. In-Ceram, Pontostar and Ti-6Al-4V alloy did not influence cell viability. Copper (52-64%), and Remanium CS (17-20%) were found to be cytotoxic, compared to control cultures. In-Ceram, Pontostar and Ti-6Al-4V had no significant influence on IL-1 (b)  secretion. Remanium-CS increased IL-1(b)  levels two fold compared to untreated control cultures. Our findings suggested that Ti-6Al-4V, In-Ceram and Pontostar did not cause elevated IL-1 (b)  release from cells at non-toxic levels. On the other hand, Remanium-CS showed moderate toxicity and caused increase in IL-1 (b)  levels.

Makale detayları

Influence of Dental Alloys and an All-Ceramic Material on Cell Viability and Interleukin-1beta Release in a Three-Dimensional Cell Culture Model

Yazarlar:

Jülide ÖZEN | Ali Uğur URAL | Mehmet DALKIZ | Bedri BEYDEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2005 Vol.35 Issue 4, pp.203-208

Belge Tipi:

Makale

Yayın Tarihi:

3/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000756347

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Cell viability

MTT

IL-1 (b)

Dental alloys

Three-dimensional cell culture

Sayı

Makale detayları

Influence of Dental Alloys and an All-Ceramic Material on Cell Viability and Interleukin-1beta Release in a Three-Dimensional Cell Culture Model

Yazarlar: Jülide ÖZEN | Ali Uğur URAL | Mehmet DALKIZ | Bedri BEYDEMİR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2005 Vol.35 Issue 4, pp.203-208

Belge Tipi: Makale

Yayın Tarihi: 3/2005

Belge Dili: Türkçe

Document ID (Org No.): 2000756347

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Cell viability

MTT

IL-1 (b)

Dental alloys

Three-dimensional cell culture

Sayı

Yazarlar:

Jülide ÖZEN | Ali Uğur URAL | Mehmet DALKIZ | Bedri BEYDEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2005 Vol.35 Issue 4, pp.203-208

Belge Tipi:

Makale

Yayın Tarihi:

3/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000756347

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144