High levels of polyphosphate kinase affect not only endotoxin production but also acid tolerance of Bacillus thuringiensis

TUĞRUL DORUK; ZEYNEP GİRGİN ERSOY; MEHMET SALİM ÖNCEL; SEDEF TUNCA GEDİK

Makale detayları

High levels of polyphosphate kinase affect not only endotoxin production but also acid tolerance of Bacillus thuringiensis

Yazarlar:

TUĞRUL DORUK | ZEYNEP GİRGİN ERSOY | MEHMET SALİM ÖNCEL | SEDEF TUNCA GEDİK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 6, pp.1168-1177

Belge Tipi:

Makale

Yayın Tarihi:

11/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811513

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Inorganic phosphate is stored as a polyphosphate (polyP) polymer inside every living cell. This polymer is synthesized by the polyP kinase (PPK) enzyme using the terminal phosphate of ATP as substrate and it performs important functions in the cell. In this study, effects of high levels of PPK on Bacillus thuringiensis subsp. israelensis were analyzed. Recombinant Bti ppk, a PPK overproducer, was found to uptake more phosphate into the cell and produce a consistently higher amount of endotoxin than the wild type under culture conditions including a range of temperatures (25 °C, 30 °C, and 37 °C), pH values (pH 5, 6, 8, and 9), and carbon sources (maltose, mannitol, sucrose, and starch). Moreover, this strain was found to overexpress sigB, which might cause a significant increase in the acid tolerance of this microorganism. Spores of Bti ppk were found to be smaller compared to wild-type spores; however, bioassay experiments with third-instar wild Culex pipiens larvae proved that high toxicity is not the result of small spore size. This hypertoxic recombinant Bti strain is very useful for industrial applications, not only because it produces more endotoxin than the wild type under different culture conditions, but also because it is more acid-tolerant under the conditions tested.

Makale detayları

High levels of polyphosphate kinase affect not only endotoxin production but also acid tolerance of Bacillus thuringiensis

Yazarlar: TUĞRUL DORUK | ZEYNEP GİRGİN ERSOY | MEHMET SALİM ÖNCEL | SEDEF TUNCA GEDİK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 6, pp.1168-1177

Belge Tipi: Makale

Yayın Tarihi: 11/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000811513

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

TUĞRUL DORUK | ZEYNEP GİRGİN ERSOY | MEHMET SALİM ÖNCEL | SEDEF TUNCA GEDİK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 6, pp.1168-1177

Belge Tipi:

Makale

Yayın Tarihi:

11/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811513

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152