The Effect of Ceftriaxone on Penicillin-Induced Epileptiform Activity in Rats: An Electrophysiological Study

Zeynep Kasap Acungil; Şeyma Özsoy

Makale detayları

The Effect of Ceftriaxone on Penicillin-Induced Epileptiform Activity in Rats: An Electrophysiological Study

Yazarlar:

Zeynep Kasap Acungil | Şeyma Özsoy

Yayıncı:

Sakarya University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Sakarya Tıp Dergisi Vol.14 Issue 1, pp.34-46

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001312771

pISSN:

2146-409X

Anahtar kelimeler:

Özet:

 Purpose: Epilepsy is a set of chronic neurological disorders characterized by seizures associated with abnormal and uncontrolled neuronal activity of the brain. Glutamate is the main excitatory neurotransmitter in the central nervous system. Excitatory amino acid transporter-2 EAAT2, one of the major glutamate transporters, is responsible for total glutamate intake. Ceftriaxone is a β-lactam antibiotic that increases EAAT-2 expression and functional activity. This study aims to investigate the effects of ceftriaxone on penicillin- induced epileptiform activity by using electrocorticography ECoG in anesthetized rats. Method: In this study, 35 Wistar male rats were used. The rats were divided into five groups of 7. In group 1, 2.5 μL 500 IU of penicillin intracranially i.c. and 1 ml saline solution and intraperitoneally i.p. were given, respectively. In group 2, 200 mgkg, i.p. of ceftriaxone was administered 30 minutes after penicillin. In group 3, 400 mgkg of ceftriaxone was administered i.p. 30 minutes after penicillin. 500 mgkg of sodium valproate was administered i.p. following 30 minutes of penicillin in group 4. In group 5, 400 mgkg, i.p. of ceftriaxone and 500 mgkg, i.p. of sodium valproate were administered 30 minutes after penicillin. After the surgical procedure the rats were placed in a stereotaxic device and electrocorticogram recordings were captured for 210 minutes. Results: The acute treatment of ceftriaxone reduced spike-wave frequency and spike-wave amplitude of penicillin-induced epileptiform activity in the rats. Conclusion: These findings suggest that acute ceftriaxone had an anticonvulsant effect on penicillin-induced focal onset epileptic activity. Ceftriaxone may have an anti-epileptogenic potential.Materials and Methods: In this study, 35 Wistar male rats were used. The rats were divided into five groups of 7. In group 1, 2.5 μL 500 IU of penicillin intracranially i.c. and 1 ml saline solution and intraperitoneally i.p. were given, respectively. In group 2, 200 mgkg, i.p. of ceftriaxone was administered 30 minutes after penicillin. In group 3, 400 mgkg of ceftriaxone was administered i.p. 30 minutes after penicillin. 500 mgkg of sodium valproate was administered i.p. following 30 minutes of penicillin in group 4. In group 5, 400 mgkg, i.p. of ceftriaxone and 500 mgkg, i.p. of sodium valproate were administered 30 minutes after penicillin. The rats were placed in a stereotaxic device after the procedure, and electrocorticogram recordings were captured for 180 minutes. Results: The acute treatment of ceftriaxone reduced spike-wave frequency and spike-wave amplitude of penicillin-induced epileptiform activity in the rats. Conclusion: These findings suggest that acute ceftriaxone had an anticonvulsant effect on penicillin-induced focal onset epileptic activity. Ceftriaxone may has an anti-epileptogenic potential.

 Purpose: Epilepsy is a set of chronic neurological disorders characterized by seizures associated with abnormal and uncontrolled neuronal activity of the brain. Glutamate is the main excitatory neurotransmitter in the central nervous system. Excitatory amino acid transporter-2 EAAT2, one of the major glutamate transporters, is responsible for total glutamate intake. Ceftriaxone is a β-lactam antibiotic that increases EAAT-2 expression and functional activity. This study aims to investigate the effects of ceftriaxone on penicillin- induced epileptiform activity by using electrocorticography ECoG in anesthetized rats. Method: In this study, 35 Wistar male rats were used. The rats were divided into five groups of 7. In group 1, 2.5 μL 500 IU of penicillin intracranially i.c. and 1 ml saline solution and intraperitoneally i.p. were given, respectively. In group 2, 200 mgkg, i.p. of ceftriaxone was administered 30 minutes after penicillin. In group 3, 400 mgkg of ceftriaxone was administered i.p. 30 minutes after penicillin. 500 mgkg of sodium valproate was administered i.p. following 30 minutes of penicillin in group 4. In group 5, 400 mgkg, i.p. of ceftriaxone and 500 mgkg, i.p. of sodium valproate were administered 30 minutes after penicillin. After the surgical procedure the rats were placed in a stereotaxic device and electrocorticogram recordings were captured for 210 minutes. Results: The acute treatment of ceftriaxone reduced spike-wave frequency and spike-wave amplitude of penicillin-induced epileptiform activity in the rats. Conclusion: These findings suggest that acute ceftriaxone had an anticonvulsant effect on penicillin-induced focal onset epileptic activity. Ceftriaxone may have an anti-epileptogenic potential.Materials and Methods: In this study, 35 Wistar male rats were used. The rats were divided into five groups of 7. In group 1, 2.5 μL 500 IU of penicillin intracranially i.c. and 1 ml saline solution and intraperitoneally i.p. were given, respectively. In group 2, 200 mgkg, i.p. of ceftriaxone was administered 30 minutes after penicillin. In group 3, 400 mgkg of ceftriaxone was administered i.p. 30 minutes after penicillin. 500 mgkg of sodium valproate was administered i.p. following 30 minutes of penicillin in group 4. In group 5, 400 mgkg, i.p. of ceftriaxone and 500 mgkg, i.p. of sodium valproate were administered 30 minutes after penicillin. The rats were placed in a stereotaxic device after the procedure, and electrocorticogram recordings were captured for 180 minutes. Results: The acute treatment of ceftriaxone reduced spike-wave frequency and spike-wave amplitude of penicillin-induced epileptiform activity in the rats. Conclusion: These findings suggest that acute ceftriaxone had an anticonvulsant effect on penicillin-induced focal onset epileptic activity. Ceftriaxone may has an anti-epileptogenic potential.

 Purpose: Epilepsy is a set of chronic neurological disorders characterized by seizures associated with abnormal and uncontrolled neuronal activity of the brain. Glutamate is the main excitatory neurotransmitter in the central nervous system. Excitatory amino acid transporter-2 EAAT2, one of the major glutamate transporters, is responsible for total glutamate intake. Ceftriaxone is a β-lactam antibiotic that increases EAAT-2 expression and functional activity. This study aims to investigate the effects of ceftriaxone on penicillin- induced epileptiform activity by using electrocorticography ECoG in anesthetized rats. Method: In this study, 35 Wistar male rats were used. The rats were divided into five groups of 7. In group 1, 2.5 μL 500 IU of penicillin intracranially i.c. and 1 ml saline solution and intraperitoneally i.p. were given, respectively. In group 2, 200 mgkg, i.p. of ceftriaxone was administered 30 minutes after penicillin. In group 3, 400 mgkg of ceftriaxone was administered i.p. 30 minutes after penicillin. 500 mgkg of sodium valproate was administered i.p. following 30 minutes of penicillin in group 4. In group 5, 400 mgkg, i.p. of ceftriaxone and 500 mgkg, i.p. of sodium valproate were administered 30 minutes after penicillin. After the surgical procedure the rats were placed in a stereotaxic device and electrocorticogram recordings were captured for 210 minutes. Results: The acute treatment of ceftriaxone reduced spike-wave frequency and spike-wave amplitude of penicillin-induced epileptiform activity in the rats. Conclusion: These findings suggest that acute ceftriaxone had an anticonvulsant effect on penicillin-induced focal onset epileptic activity. Ceftriaxone may have an anti-epileptogenic potential.Materials and Methods: In this study, 35 Wistar male rats were used. The rats were divided into five groups of 7. In group 1, 2.5 μL 500 IU of penicillin intracranially i.c. and 1 ml saline solution and intraperitoneally i.p. were given, respectively. In group 2, 200 mgkg, i.p. of ceftriaxone was administered 30 minutes after penicillin. In group 3, 400 mgkg of ceftriaxone was administered i.p. 30 minutes after penicillin. 500 mgkg of sodium valproate was administered i.p. following 30 minutes of penicillin in group 4. In group 5, 400 mgkg, i.p. of ceftriaxone and 500 mgkg, i.p. of sodium valproate were administered 30 minutes after penicillin. The rats were placed in a stereotaxic device after the procedure, and electrocorticogram recordings were captured for 180 minutes. Results: The acute treatment of ceftriaxone reduced spike-wave frequency and spike-wave amplitude of penicillin-induced epileptiform activity in the rats. Conclusion: These findings suggest that acute ceftriaxone had an anticonvulsant effect on penicillin-induced focal onset epileptic activity. Ceftriaxone may has an anti-epileptogenic potential.

 Amaç: Epilepsi, beynin anormal ve koordinesiz nöronal aktivitesi ile ilişkili nöbetlerle karakterize bir dizi kronik nörolojik bozukluktur. Glutamat, merkezi sinir sistemindeki ana uyarıcı nörotransmitterdir. Başlıca glutamat taşıyıcılarından biri olan eksitatör amino asit taşıyıcı-2 EAAT2, toplam glutamat alımından sorumludur. Seftriakson, EAAT-2 ekspresyonunu ve fonksiyonel aktivitesini artıran bir β-laktam antibiyotiktir. Bu çalışmanın amacı, seftriaksonun penisilin ile indüklenen epileptiform aktivite üzerindeki etkilerini anestezi uygulanmış sıçanlarda elektrokortikografi ECoG kullanarak araştırmaktır. Yöntem ve Gereçler: Bu çalışmada 35 Wistar erkek sıçan kullanıldı. Sıçanlar 7şerli beş gruba ayrıldı. Grup 1e sırasıyla 2.5 μL 500 IU penisilin intrakraniyal i.c. ve 1 ml salin solüsyonu intraperitoneal i.p. olarak verildi. Grup 2de penisilinden 30 dakika sonra 200 mgkg, i.p. seftriakson uygulandı. Grup 3te penisilinden 30 dakika sonra 400 mgkg seftriakson i.p. olarak uygulandı. Grup 4te penisilinden 30 dakika sonra 500 mgkg sodyum valproat i.p. olarak uygulandı. Grup 5te penisilinden 30 dakika sonra 400 mgkg i.p. seftriakson ve 500 mgkg i.p. sodyum valproat uygulanmıştır. Sıçanlar işlemden sonra stereotaksik cihaza yerleştirildi ve 180 dakika boyunca elektrokortikogram kayıtları alındı. Bulgular: Seftriaksonun akut tedavisi sıçanlarda penisilin ile indüklenen epileptiform aktivitenin diken dalga frekansını ve diken dalga genliğini azaltmıştır. Sonuç: Bu bulgular, akut seftriaksonun penisilin ile indüklenen fokal başlangıçlı epileptik aktivite üzerinde antikonvülsan bir etkiye sahip olabileceğini göstermektedir. Seftriaksonun anti-epileptojenik bir potansiyeli olabilir.

Makale detayları

The Effect of Ceftriaxone on Penicillin-Induced Epileptiform Activity in Rats: An Electrophysiological Study

Yazarlar:

Zeynep Kasap Acungil | Şeyma Özsoy

Yayıncı:

Sakarya University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Sakarya Tıp Dergisi Vol.14 Issue 1, pp.34-46

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001312771

pISSN:

2146-409X

Experimental epilepsy

Ceftriaxone

Electrocorticographydeneysel epilepsi

seftriakson

elektrokortikografi

Sayı

Makale detayları

The Effect of Ceftriaxone on Penicillin-Induced Epileptiform Activity in Rats: An Electrophysiological Study

Yazarlar: Zeynep Kasap Acungil | Şeyma Özsoy

Yayıncı: Sakarya University

Ana Başlıklar: Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi: Sakarya Tıp Dergisi Vol.14 Issue 1, pp.34-46

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001312771

pISSN: 2146-409X

Experimental epilepsy

Ceftriaxone

Electrocorticographydeneysel epilepsi

seftriakson

elektrokortikografi

Sayı

Yazarlar:

Zeynep Kasap Acungil | Şeyma Özsoy

Yayıncı:

Sakarya University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Sakarya Tıp Dergisi Vol.14 Issue 1, pp.34-46

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001312771

pISSN:

2146-409X