In situ Localisation of Axi 1 (Auxin Independent) Gene in Rhizobium leguminosarum  Inoculated Root Tips of Pea Plants

Yeşim YALÇIN MENDİ; Katharina PAWLOWSKI; Selim ÇETİNER

Makale detayları

In situ Localisation of Axi 1 (Auxin Independent) Gene in Rhizobium leguminosarum Inoculated Root Tips of Pea Plants

Yazarlar:

Yeşim YALÇIN MENDİ | Katharina PAWLOWSKI | Selim ÇETİNER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2001 Vol.25 Issue 4, pp.265-271

Belge Tipi:

Makale

Yayın Tarihi:

3/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000809592

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Axi 1 is the only  characterized auxin independent gene in tobacco plants.  In this study, the effect of axi 1 gene on cortical cell division during different stages of nitrogen fixation and on the role of polar auxin transport  were investigated. Root and nodule samples were taken from pea seedlings 24 hours, 48 hours, 6 days and 10 days after inoculation with R. leguminosarum PRE strain. Using in  situ hybridisation, the location of the axi 1 gene expression in root and nodule tissues was identified. The results showed that in root tissues inoculated with Rhizobia, the axi 1 gene is  expressed on the phloem tissue of the vascular  bundle. In the 10-day-old nodule tissues, the gene expression was found in the phloem tissue, around the nodule bundle and nodule meristematic region. The results obtained indicate that also Axi 1 gene plays a role in nitrogen fixation and auxine transport.

 

Axi 1 is the only  characterized auxin independent gene in tobacco plants.  In this study, the effect of axi 1 gene on cortical cell division during different stages of nitrogen fixation and on the role of polar auxin transport  were investigated. Root and nodule samples were taken from pea seedlings 24 hours, 48 hours, 6 days and 10 days after inoculation with R. leguminosarum PRE strain. Using in  situ hybridisation, the location of the axi 1 gene expression in root and nodule tissues was identified. The results showed that in root tissues inoculated with Rhizobia, the axi 1 gene is  expressed on the phloem tissue of the vascular  bundle. In the 10-day-old nodule tissues, the gene expression was found in the phloem tissue, around the nodule bundle and nodule meristematic region. The results obtained indicate that also Axi 1 gene plays a role in nitrogen fixation and auxine transport.

Makale detayları

In situ Localisation of Axi 1 (Auxin Independent) Gene in Rhizobium leguminosarum Inoculated Root Tips of Pea Plants

Yazarlar: Yeşim YALÇIN MENDİ | Katharina PAWLOWSKI | Selim ÇETİNER

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2001 Vol.25 Issue 4, pp.265-271

Belge Tipi: Makale

Yayın Tarihi: 3/2001

Belge Dili: Türkçe

Document ID (Org No.): 2000809592

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Axi 1

gene expression

pea

nodule

Sayı

Yazarlar:

Yeşim YALÇIN MENDİ | Katharina PAWLOWSKI | Selim ÇETİNER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2001 Vol.25 Issue 4, pp.265-271

Belge Tipi:

Makale

Yayın Tarihi:

3/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000809592

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X