Evaluation of the Anticarcinogenic Effect of White Radish Extract (Raphanus sativus var. Longipinnatus) on In Vitro Ehrlich Ascites Tumor Cells

Sümeyye Uçar; Aslı Okan Oflamaz; Mert Ocak

Makale detayları

Evaluation of the Anticarcinogenic Effect of White Radish Extract (Raphanus sativus var. Longipinnatus) on In Vitro Ehrlich Ascites Tumor Cells

Yazarlar:

Sümeyye Uçar | Aslı Okan Oflamaz | Mert Ocak

Yayıncı:

Gumushane University

Ana Başlıklar:

Hücre Metabolizması

Dergi:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.13 Issue 3, pp.1003-1008

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328036

pISSN:

2146-9954

Anahtar kelimeler:

Özet:

 In this research, it was aimed to research the time and different dose effects of white radish extract Raphanus sativus var. Longipinnatus on Ehrlich Ascites Tumor EAT cell lines. EAT cell line was used in the study. EAT cells were treated white radish extract Raphanus sativus var. Longipinnatus at 37C and 5 CO2 for differing periods 24 and 48 hours and doses 100-200 and 300 μgml white radish extract. At the end of the incubation duration, Argyrophilic nucleolar organizing region AgNOR protein condition of EAT cells were investigated.It was determined among the control and 300 μgml Raphanus sativus extract group the significant differences for mean AgNOR number and TAANA in 48 hours period. It was detected also between the 100 and 300 μgml Raphanus sativus extract groups for AgNOR number and TAANA in 48 hours incubation. This study demonstrated that Raphanus sativus had a important role against cancer cells. Also, both AgNOR values mit be used as biomarkers for identification of the most true therapeutic dose option for cancer and it has been shown that suitable ingestion of Raphanus sativus can be effective in avoid cancer development and slowing its spreading.

 In this research, it was aimed to research the time and different dose effects of white radish extract Raphanus sativus var. Longipinnatus on Ehrlich Ascites Tumor EAT cell lines. EAT cell line was used in the study. EAT cells were treated white radish extract Raphanus sativus var. Longipinnatus at 37C and 5 CO2 for differing periods 24 and 48 hours and doses 100-200 and 300 μgml white radish extract. At the end of the incubation duration, Argyrophilic nucleolar organizing region AgNOR protein condition of EAT cells were investigated.It was determined among the control and 300 μgml Raphanus sativus extract group the significant differences for mean AgNOR number and TAANA in 48 hours period. It was detected also between the 100 and 300 μgml Raphanus sativus extract groups for AgNOR number and TAANA in 48 hours incubation. This study demonstrated that Raphanus sativus had a important role against cancer cells. Also, both AgNOR values mit be used as biomarkers for identification of the most true therapeutic dose option for cancer and it has been shown that suitable ingestion of Raphanus sativus can be effective in avoid cancer development and slowing its spreading.

 In this research, it was aimed to research the time and different dose effects of white radish extract Raphanus sativus var. Longipinnatus on Ehrlich Ascites Tumor EAT cell lines. EAT cell line was used in the study. EAT cells were treated white radish extract Raphanus sativus var. Longipinnatus at 37C and 5 CO2 for differing periods 24 and 48 hours and doses 100-200 and 300 μgml white radish extract. At the end of the incubation duration, Argyrophilic nucleolar organizing region AgNOR protein condition of EAT cells were investigated.It was determined among the control and 300 μgml Raphanus sativus extract group the significant differences for mean AgNOR number and TAANA in 48 hours period. It was detected also between the 100 and 300 μgml Raphanus sativus extract groups for AgNOR number and TAANA in 48 hours incubation. This study demonstrated that Raphanus sativus had a important role against cancer cells. Also, both AgNOR values mit be used as biomarkers for identification of the most true therapeutic dose option for cancer and it has been shown that suitable ingestion of Raphanus sativus can be effective in avoid cancer development and slowing its spreading.

 Bu çalışmada beyaz turp Raphanus sativus var. Longipinnatus ekstresinin Ehrlich Assit Tümör EAT hücre hattı üzerinde zaman ve doza bağlı etkilerinin incelenmesi amaçlanmıştır. Çalışmada EAT hücre hattı kullanıldı. EAT hücreleri, değişen sürelerde 24 ve 48 saat ve dozlarda 100-200 ve 300 μgml beyaz turp ekstresi 37C ve 5 CO2de beyaz turp ekstresine Raphanus sativus var. Longipinnatus maruz bırakıldı. İnkübasyon süresinin sonunda EAT hücrelerinin argyrophilic nükleolar organize bölge AgNOR protein durumu incelendi. 48 saatlik inkübasyonda ortalama AgNOR sayısı ve TAANA açısından kontrol ve 300 μgml Raphanus sativus ekstresi grubu arasında anlamlı fark bulundu. Ayrıca 48 saatlik inkübasyonda AgNOR sayısı ve TAANA için 100 ve 300 μgml Raphanus sativus ekstre gruplarında istatistiksel olarak fark görüldü p0,05. Mevcut çalışmamızda, Raphanus sativusun kanser gelişimine karşı çok önemli bir işlevi olduğunu göstermiştir. Ayrıca, AgNOR kanser için en güvenilir terapötik doz seçiminin saptanmasında biyobelirteç olarak kullanılmaktadır. Raphanus sativusun doğru tüketiminin kanser oluşumunu önlemede ve ilerlemesini yavaşlatmada etkili olabileceği gösterilmiştir.

Makale detayları

Evaluation of the Anticarcinogenic Effect of White Radish Extract (Raphanus sativus var. Longipinnatus) on In Vitro Ehrlich Ascites Tumor Cells

Yazarlar:

Sümeyye Uçar | Aslı Okan Oflamaz | Mert Ocak

Yayıncı:

Gumushane University

Ana Başlıklar:

Hücre Metabolizması

Dergi:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.13 Issue 3, pp.1003-1008

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328036

pISSN:

2146-9954

AgNOR

Cancer cell line

EAT

Raphanus sativus L.AgNOR

Kanser hücre hattı

EAT

Raphanus sativus L.

Sayı

Makale detayları

Yazarlar: Sümeyye Uçar | Aslı Okan Oflamaz | Mert Ocak

Yayıncı: Gumushane University

Ana Başlıklar: Hücre Metabolizması

Dergi: Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.13 Issue 3, pp.1003-1008

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328036

pISSN: 2146-9954

Sayı

Yazarlar:

Sümeyye Uçar | Aslı Okan Oflamaz | Mert Ocak

Yayıncı:

Gumushane University

Ana Başlıklar:

Hücre Metabolizması

Dergi:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.13 Issue 3, pp.1003-1008

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328036

pISSN:

2146-9954