Gibberellic Acid is Active Only in Orchid Cross-Pollination

Mehmet Aybeke

Makale detayları

Gibberellic Acid is Active Only in Orchid Cross-Pollination

Yazarlar:

Mehmet Aybeke

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Bitki Bilimi (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.8 Issue 2, pp.533-542

Belge Tipi:

Makale

Yayın Tarihi:

12/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001335204

pISSN:

2687-3729

Anahtar kelimeler:

Özet:

 The aim of this study is to determine the endogenous hormone activities of Gibberellic acid GA in compatible Intraspecific ISP and incompatible pollination Intergeneric IGP. It was designed to be Himantoglossum robertianum in ISP experiments and Himantoglossum robertianum and Orchis italica in IGP experiments. For this reason, some polynariums taken from O. italica have been applied with needles to the flower stigmas of H. robertianum. Ovaries with stigma have been taken from both types of pollination for 10 days, and quantitative hormone analyses have been performed by LC-MSMS. As a result, GA was not found in the ISP experiments. Likewise, it did not appear at all in the tests between the 1st and 6th days of IGP; it was found only on the 7th, 8th, 9th, and 10th days of IGP and at increasingly higher values. For the first time, endogenous ovary-stigma GA amounts in the post-pollination process of orchid IGP were determined in this study, and its importance was discussed. According to statistical analysis, there is a significant difference between almost all values. It has been understood that additional histological and embryological studies are needed to understand the reason for the very high activity, especially on days 9 and 10.

 The aim of this study is to determine the endogenous hormone activities of Gibberellic acid GA in compatible Intraspecific ISP and incompatible pollination Intergeneric IGP. It was designed to be Himantoglossum robertianum in ISP experiments and Himantoglossum robertianum and Orchis italica in IGP experiments. For this reason, some polynariums taken from O. italica have been applied with needles to the flower stigmas of H. robertianum. Ovaries with stigma have been taken from both types of pollination for 10 days, and quantitative hormone analyses have been performed by LC-MSMS. As a result, GA was not found in the ISP experiments. Likewise, it did not appear at all in the tests between the 1st and 6th days of IGP; it was found only on the 7th, 8th, 9th, and 10th days of IGP and at increasingly higher values. For the first time, endogenous ovary-stigma GA amounts in the post-pollination process of orchid IGP were determined in this study, and its importance was discussed. According to statistical analysis, there is a significant difference between almost all values. It has been understood that additional histological and embryological studies are needed to understand the reason for the very high activity, especially on days 9 and 10.

 The aim of this study is to determine the endogenous hormone activities of Gibberellic acid GA in compatible Intraspecific ISP and incompatible pollination Intergeneric IGP. It was designed to be Himantoglossum robertianum in ISP experiments and Himantoglossum robertianum and Orchis italica in IGP experiments. For this reason, some polynariums taken from O. italica have been applied with needles to the flower stigmas of H. robertianum. Ovaries with stigma have been taken from both types of pollination for 10 days, and quantitative hormone analyses have been performed by LC-MSMS. As a result, GA was not found in the ISP experiments. Likewise, it did not appear at all in the tests between the 1st and 6th days of IGP; it was found only on the 7th, 8th, 9th, and 10th days of IGP and at increasingly higher values. For the first time, endogenous ovary-stigma GA amounts in the post-pollination process of orchid IGP were determined in this study, and its importance was discussed. According to statistical analysis, there is a significant difference between almost all values. It has been understood that additional histological and embryological studies are needed to understand the reason for the very high activity, especially on days 9 and 10.

 Bu çalışmanın amacı Gibberellik asitin GA uyumlu IntraspesifikISP ve uyumsuz tozlaşmada IntergenericIGP endojen hormon aktivitelerini belirlemektir. ISP deneylerinde Himantoglossum robertianum ve IGP deneylerinde Himantoglossum robertianum ve Orchis italica olacak şekilde tasarlandı. Bu nedenle O. italicadan alınan bazı polinariumlar H. robertianumun çiçek stigmalarına iğnelerle uygulanmıştır. Her iki tozlaşma türünden de stigmalı yumurtalıklar 10 gün süreyle alınmış ve LC-MSMS ile kantitatif hormon analizleri yapılmıştır. Sonuç olarak ISP deneylerinde GA bulunamadı. Aynı şekilde IGPnin 1. ve 6. günleri arasında yapılan testlerde de hiç görünmedi; IGPnin sadece 7, 8, 9 ve 10. günlerinde ve giderek daha yüksek değerlerde bulundu. İlk kez bu çalışmada orkide IGPnin tozlaşma sonrası süreçteki endojen yumurtalık-stigma GA miktarları belirlenmiş ve önemi tartışılmıştır. İstatistiksel analizlere göre hemen hemen tüm değerler arasında anlamlı bir fark vardır. Özellikle 9. ve 10. günlerde görülen çok yüksek aktivitenin nedeninin anlaşılabilmesi için ilave histolojik ve embriyolojik çalışmalara ihtiyaç duyulduğu anlaşılmıştır.

Makale detayları

Gibberellic Acid is Active Only in Orchid Cross-Pollination

Yazarlar:

Mehmet Aybeke

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Bitki Bilimi (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.8 Issue 2, pp.533-542

Belge Tipi:

Makale

Yayın Tarihi:

12/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001335204

pISSN:

2687-3729

Himantoglossum

hormone

orchide

post polination phenomena

flowerHimantoglossum

hormon

orkide

tozlaşma sonrası olaylar

çiçek

Sayı

Makale detayları

Yazarlar: Mehmet Aybeke

Yayıncı: Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar: Bitki Bilimi (Diğer)

Dergi: Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.8 Issue 2, pp.533-542

Belge Tipi: Makale

Yayın Tarihi: 12/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001335204

pISSN: 2687-3729

Sayı

Yazarlar:

Mehmet Aybeke

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Bitki Bilimi (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.8 Issue 2, pp.533-542

Belge Tipi:

Makale

Yayın Tarihi:

12/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001335204

pISSN:

2687-3729