Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins

Ely ZAYOVA; Ira STANCHEVA; Maria GENEVA; Maria PETROVA; Lyudmila DIMITROVA

Makale detayları

Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins

Yazarlar:

Ely ZAYOVA | Ira STANCHEVA | Maria GENEVA | Maria PETROVA | Lyudmila DIMITROVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 1, pp.106-113

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812530

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

An efficient in vitro protocol for propagation of Stevia rebaudiana Bertoni is described. Multiple shoots were induced in vitro from shoot tip and nodal segments on Murashige and Skoog medium containing 6-benzylaminopurine, zeatin, or thidiazuron alone and in combination with naphthalene acetic acid or indole-3-acetic acid. A high frequency of shoot induction as well as maximum number of shoots per shoot tip explant was observed on Murashige and Skoog medium supplemented with 6-benzylaminopurine (1.0 mg L-1) alone and combined with indole-3-acetic acid (0.1 mg L-1). For root induction, in vitro shoots were transferred to rooting media containing naphthalene acetic acid, indole-3-acetic acid, or indole-3-butyric acid. The highest rooting frequency and the highest number of roots was observed in half-strength Murashige and Skoog medium supplemented with 0.1 mg L-1 indole-3-butyric acid. The rooted in vitro plants were successfully acclimatized in a growth chamber and transferred to the field. Leaf extracts of plants propagated in vitro and adapted to field conditions are characterized by high levels of water-soluble antioxidant capacity (expressed as equivalents of ascorbic acid), phenols, and flavonoids, and therefore by high total antioxidant potential, expressed as DPPH radical scavenging activity.

 

An efficient in vitro protocol for propagation of Stevia rebaudiana Bertoni is described. Multiple shoots were induced in vitro from shoot tip and nodal segments on Murashige and Skoog medium containing 6-benzylaminopurine, zeatin, or thidiazuron alone and in combination with naphthalene acetic acid or indole-3-acetic acid. A high frequency of shoot induction as well as maximum number of shoots per shoot tip explant was observed on Murashige and Skoog medium supplemented with 6-benzylaminopurine (1.0 mg L-1) alone and combined with indole-3-acetic acid (0.1 mg L-1). For root induction, in vitro shoots were transferred to rooting media containing naphthalene acetic acid, indole-3-acetic acid, or indole-3-butyric acid. The highest rooting frequency and the highest number of roots was observed in half-strength Murashige and Skoog medium supplemented with 0.1 mg L-1 indole-3-butyric acid. The rooted in vitro plants were successfully acclimatized in a growth chamber and transferred to the field. Leaf extracts of plants propagated in vitro and adapted to field conditions are characterized by high levels of water-soluble antioxidant capacity (expressed as equivalents of ascorbic acid), phenols, and flavonoids, and therefore by high total antioxidant potential, expressed as DPPH radical scavenging activity.

Makale detayları

Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins

Yazarlar:

Ely ZAYOVA | Ira STANCHEVA | Maria GENEVA | Maria PETROVA | Lyudmila DIMITROVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 1, pp.106-113

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812530

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Acclimatization

micropropagation

nodal segments

shoot tips

Sayı

Makale detayları

Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins

Yazarlar: Ely ZAYOVA | Ira STANCHEVA | Maria GENEVA | Maria PETROVA | Lyudmila DIMITROVA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 1, pp.106-113

Belge Tipi: Makale

Yayın Tarihi: 12/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000812530

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Acclimatization

micropropagation

nodal segments

shoot tips

Sayı

Yazarlar:

Ely ZAYOVA | Ira STANCHEVA | Maria GENEVA | Maria PETROVA | Lyudmila DIMITROVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 1, pp.106-113

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812530

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152