Investigation of Lactobacillus spp. bacteria in infants consuming breast milk and formula and determination of some probiotic characters

Tuğçe Muslu Çağal; Esin Kıray; Ergin Kariptaş

Makale detayları

Investigation of Lactobacillus spp. bacteria in infants consuming breast milk and formula and determination of some probiotic characters

Yazarlar:

Tuğçe Muslu Çağal | Esin Kıray | Ergin Kariptaş

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Medical Microbiology

Dergi:

The European Research Journal Vol.9 Issue 6, pp.1334-1342

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294096

pISSN:

2149-3189

Anahtar kelimeler:

Özet:

 Objectives: It was aimed to investigate some probiotic properties of Lactic Acid Bacteria LAB isolated from stool samples taken from 45 healthy 0-4 month old babies, who did not take antibiotics or probiotic supplements in the last 3 months, and who did not have any health problems. Methods: Six different species were obtained from 21 isolates selected by the method of Mass Spectrometry MALDI-TOF MS. Results: The most common strain was Lactobacillus rhamnosus with a rate of 59, followed by Lactobacillus paracasei with a rate of 13.6. Vancomycin, tetracycline, gentamicin, netilmicin, tobramycin, penicillin, ampicillin, teicoplanin and amikacin antibiotics were used to evaluate the antimicrobial activities of the strains. In our study, while all strains were resistant to the antibiotic amikacin, they were sensitive to tetracycline, penicillin, gentamicin, netilmicin, teicoplanin, vancomycin, ampicillin and tobramycin antibiotics. In the evaluation of the antagonistic activities of LAB, 6 different pathogens  Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, S. aureus ATCC 25923, Candida albicans ATCC 10231, Listeria monocytogenes ATCC 19111 were used and it was determined that the strains showed antimicrobial effects on all pathogenic microorganisms. Cholesterol assimilation abilities, T21 and T22 strains achieved the highest cholesterol assimilation rate of 39.1. Conclusions: It is thought that most of the isolated strains have probiotic potential, and especially Lactobacillus gasseri T21 and L. paracasei T22 strains may be probiotic strains that can be used in the production of preparations alone or together with other Lactobacillus strains .

 Objectives: It was aimed to investigate some probiotic properties of Lactic Acid Bacteria LAB isolated from stool samples taken from 45 healthy 0-4 month old babies, who did not take antibiotics or probiotic supplements in the last 3 months, and who did not have any health problems. Methods: Six different species were obtained from 21 isolates selected by the method of Mass Spectrometry MALDI-TOF MS. Results: The most common strain was Lactobacillus rhamnosus with a rate of 59, followed by Lactobacillus paracasei with a rate of 13.6. Vancomycin, tetracycline, gentamicin, netilmicin, tobramycin, penicillin, ampicillin, teicoplanin and amikacin antibiotics were used to evaluate the antimicrobial activities of the strains. In our study, while all strains were resistant to the antibiotic amikacin, they were sensitive to tetracycline, penicillin, gentamicin, netilmicin, teicoplanin, vancomycin, ampicillin and tobramycin antibiotics. In the evaluation of the antagonistic activities of LAB, 6 different pathogens  Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, S. aureus ATCC 25923, Candida albicans ATCC 10231, Listeria monocytogenes ATCC 19111 were used and it was determined that the strains showed antimicrobial effects on all pathogenic microorganisms. Cholesterol assimilation abilities, T21 and T22 strains achieved the highest cholesterol assimilation rate of 39.1. Conclusions: It is thought that most of the isolated strains have probiotic potential, and especially Lactobacillus gasseri T21 and L. paracasei T22 strains may be probiotic strains that can be used in the production of preparations alone or together with other Lactobacillus strains .

 Objectives: It was aimed to investigate some probiotic properties of Lactic Acid Bacteria LAB isolated from stool samples taken from 45 healthy 0-4 month old babies, who did not take antibiotics or probiotic supplements in the last 3 months, and who did not have any health problems. Methods: Six different species were obtained from 21 isolates selected by the method of Mass Spectrometry MALDI-TOF MS. Results: The most common strain was Lactobacillus rhamnosus with a rate of 59, followed by Lactobacillus paracasei with a rate of 13.6. Vancomycin, tetracycline, gentamicin, netilmicin, tobramycin, penicillin, ampicillin, teicoplanin and amikacin antibiotics were used to evaluate the antimicrobial activities of the strains. In our study, while all strains were resistant to the antibiotic amikacin, they were sensitive to tetracycline, penicillin, gentamicin, netilmicin, teicoplanin, vancomycin, ampicillin and tobramycin antibiotics. In the evaluation of the antagonistic activities of LAB, 6 different pathogens  Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, S. aureus ATCC 25923, Candida albicans ATCC 10231, Listeria monocytogenes ATCC 19111 were used and it was determined that the strains showed antimicrobial effects on all pathogenic microorganisms. Cholesterol assimilation abilities, T21 and T22 strains achieved the highest cholesterol assimilation rate of 39.1. Conclusions: It is thought that most of the isolated strains have probiotic potential, and especially Lactobacillus gasseri T21 and L. paracasei T22 strains may be probiotic strains that can be used in the production of preparations alone or together with other Lactobacillus strains .

 Objectives: It was aimed to investigate some probiotic properties of Lactic Acid Bacteria LAB isolated from stool samples taken from 45 healthy 0-4 month old babies, who did not take antibiotics or probiotic supplements in the last 3 months, and who did not have any health problems. Methods: Six different species were obtained from 21 isolates selected by the method of Mass Spectrometry MALDI-TOF MS. Results: The most common strain was Lactobacillus rhamnosus with a rate of 59, followed by Lactobacillus paracasei with a rate of 13.6. Vancomycin, tetracycline, gentamicin, netilmicin, tobramycin, penicillin, ampicillin, teicoplanin and amikacin antibiotics were used to evaluate the antimicrobial activities of the strains. In our study, while all strains were resistant to the antibiotic amikacin, they were sensitive to tetracycline, penicillin, gentamicin, netilmicin, teicoplanin, vancomycin, ampicillin and tobramycin antibiotics. In the evaluation of the antagonistic activities of LAB, 6 different pathogens  Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, S. aureus ATCC 25923, Candida albicans ATCC 10231, Listeria monocytogenes ATCC 19111 were used and it was determined that the strains showed antimicrobial effects on all pathogenic microorganisms. Cholesterol assimilation abilities, T21 and T22 strains achieved the highest cholesterol assimilation rate of 39.1. Conclusions: It is thought that most of the isolated strains have probiotic potential, and especially Lactobacillus gasseri T21 and L. paracasei T22 strains may be probiotic strains that can be used in the production of preparations alone or together with other Lactobacillus strains .

Makale detayları

Investigation of Lactobacillus spp. bacteria in infants consuming breast milk and formula and determination of some probiotic characters

Yazarlar:

Tuğçe Muslu Çağal | Esin Kıray | Ergin Kariptaş

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Medical Microbiology

Dergi:

The European Research Journal Vol.9 Issue 6, pp.1334-1342

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294096

pISSN:

2149-3189

Lactic acid bacteria

breast milk

probiotic

Sayı

Makale detayları

Investigation of Lactobacillus spp. bacteria in infants consuming breast milk and formula and determination of some probiotic characters

Yazarlar: Tuğçe Muslu Çağal | Esin Kıray | Ergin Kariptaş

Yayıncı: Prusa Medical Publishing

Ana Başlıklar: Medical Microbiology

Dergi: The European Research Journal Vol.9 Issue 6, pp.1334-1342

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001294096

pISSN: 2149-3189

Lactic acid bacteria

breast milk

probiotic

Sayı

Yazarlar:

Tuğçe Muslu Çağal | Esin Kıray | Ergin Kariptaş

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Medical Microbiology

Dergi:

The European Research Journal Vol.9 Issue 6, pp.1334-1342

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294096

pISSN:

2149-3189