Harnessing Molecularly Imprinted Polymers in Surface Plasmon Resonance and Quartz Crystal Microbalance for 17α-Ethinyl Estradiol Detection: A Comparative Study

Meltem Okan; Memed Duman

Makale detayları

Harnessing Molecularly Imprinted Polymers in Surface Plasmon Resonance and Quartz Crystal Microbalance for 17α-Ethinyl Estradiol Detection: A Comparative Study

Yazarlar:

Meltem Okan | Memed Duman

Yayıncı:

Hacettepe University

Ana Başlıklar:

Sensor Technology | Colloid and Surface Chemistry

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.52 Issue 6, pp.405-414

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319452

pISSN:

2687-475X

Anahtar kelimeler:

Özet:

 Estradiol is a critical hormone for reproductive health in females and males both. Monitoring estradiol levels can aid in diagnosing various conditions such as menopause, infertility and even some cancers. Estradiol is also a type of endocrine-disrupting chemical EDC that has diverse impacts on ecosystems as well as human health. In can enter the environment through agricultural runoff, sewage and industrial effluents. Adapting both perspectives, establishing biosensors for estradiol detection becomes crucial. In this study, 17-α-ethinylestradiol imprinted polymeric nanoparticles 17EE-MIPs were synthesized using mini-emulsion polymerization and characterized. Prior to consecutive Quartz Crystal Microbalance QCM based analysis, 17EE-MIPs were tested with Surface Plasmon Resonance SPR, from which the equilibrium and binding kinetic analysis as well as equilibrium isotherm models were derived. Freundlich model was found to best represent the 17EE-MIP based SPR platform. Next, 17EE-MIPs were covalently attached on the QCM crystal and different 17EE concentrations were tested consecutively without regeneration steps. Both systems yielded very high linearity with R2 values of 0.9798 and 0.9895 for SPR and QCM, respectively. The limit of detection LOD of the SPR and QCM sensor were calculated as 11.57 and 1.335 µM, respectively. Here, two sensing platforms were employed to crosscheck the performance of the 17EE-MIPs, both verified to respond to low concentrations of 17EE with high consistency.

 Estradiol is a critical hormone for reproductive health in females and males both. Monitoring estradiol levels can aid in diagnosing various conditions such as menopause, infertility and even some cancers. Estradiol is also a type of endocrine-disrupting chemical EDC that has diverse impacts on ecosystems as well as human health. In can enter the environment through agricultural runoff, sewage and industrial effluents. Adapting both perspectives, establishing biosensors for estradiol detection becomes crucial. In this study, 17-α-ethinylestradiol imprinted polymeric nanoparticles 17EE-MIPs were synthesized using mini-emulsion polymerization and characterized. Prior to consecutive Quartz Crystal Microbalance QCM based analysis, 17EE-MIPs were tested with Surface Plasmon Resonance SPR, from which the equilibrium and binding kinetic analysis as well as equilibrium isotherm models were derived. Freundlich model was found to best represent the 17EE-MIP based SPR platform. Next, 17EE-MIPs were covalently attached on the QCM crystal and different 17EE concentrations were tested consecutively without regeneration steps. Both systems yielded very high linearity with R2 values of 0.9798 and 0.9895 for SPR and QCM, respectively. The limit of detection LOD of the SPR and QCM sensor were calculated as 11.57 and 1.335 µM, respectively. Here, two sensing platforms were employed to crosscheck the performance of the 17EE-MIPs, both verified to respond to low concentrations of 17EE with high consistency.

 Estradiol is a critical hormone for reproductive health in females and males both. Monitoring estradiol levels can aid in diagnosing various conditions such as menopause, infertility and even some cancers. Estradiol is also a type of endocrine-disrupting chemical EDC that has diverse impacts on ecosystems as well as human health. In can enter the environment through agricultural runoff, sewage and industrial effluents. Adapting both perspectives, establishing biosensors for estradiol detection becomes crucial. In this study, 17-α-ethinylestradiol imprinted polymeric nanoparticles 17EE-MIPs were synthesized using mini-emulsion polymerization and characterized. Prior to consecutive Quartz Crystal Microbalance QCM based analysis, 17EE-MIPs were tested with Surface Plasmon Resonance SPR, from which the equilibrium and binding kinetic analysis as well as equilibrium isotherm models were derived. Freundlich model was found to best represent the 17EE-MIP based SPR platform. Next, 17EE-MIPs were covalently attached on the QCM crystal and different 17EE concentrations were tested consecutively without regeneration steps. Both systems yielded very high linearity with R2 values of 0.9798 and 0.9895 for SPR and QCM, respectively. The limit of detection LOD of the SPR and QCM sensor were calculated as 11.57 and 1.335 µM, respectively. Here, two sensing platforms were employed to crosscheck the performance of the 17EE-MIPs, both verified to respond to low concentrations of 17EE with high consistency.

 Estradiol is a critical hormone for reproductive health in females and males both. Monitoring estradiol levels can aid in diagnosing various conditions such as menopause, infertility and even some cancers. Estradiol is also a type of endocrine-disrupting chemical EDC that has diverse impacts on ecosystems as well as human health. In can enter the environment through agricultural runoff, sewage and industrial effluents. Adapting both perspectives, establishing biosensors for estradiol detection becomes crucial. In this study, 17-α-ethinylestradiol imprinted polymeric nanoparticles 17EE-MIPs were synthesized using mini-emulsion polymerization and characterized. Prior to consecutive Quartz Crystal Microbalance QCM based analysis, 17EE-MIPs were tested with Surface Plasmon Resonance SPR, from which the equilibrium and binding kinetic analysis as well as equilibrium isotherm models were derived. Freundlich model was found to best represent the 17EE-MIP based SPR platform. Next, 17EE-MIPs were covalently attached on the QCM crystal and different 17EE concentrations were tested consecutively without regeneration steps. Both systems yielded very high linearity with R2 values of 0.9798 and 0.9895 for SPR and QCM, respectively. The limit of detection LOD of the SPR and QCM sensor were calculated as 11.57 and 1.335 µM, respectively. Here, two sensing platforms were employed to crosscheck the performance of the 17EE-MIPs, both verified to respond to low concentrations of 17EE with high consistency.

Makale detayları

Harnessing Molecularly Imprinted Polymers in Surface Plasmon Resonance and Quartz Crystal Microbalance for 17α-Ethinyl Estradiol Detection: A Comparative Study

Yazarlar:

Meltem Okan | Memed Duman

Yayıncı:

Hacettepe University

Ana Başlıklar:

Sensor Technology | Colloid and Surface Chemistry

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.52 Issue 6, pp.405-414

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319452

pISSN:

2687-475X

17-alpha-ethinylestradiol

Molecularly Imprinted Polymers

Surface Plasmon Resonance

Quartz Crystal Microbalance

Mass Sensor

Sayı

Makale detayları

Harnessing Molecularly Imprinted Polymers in Surface Plasmon Resonance and Quartz Crystal Microbalance for 17α-Ethinyl Estradiol Detection: A Comparative Study

Yazarlar: Meltem Okan | Memed Duman

Yayıncı: Hacettepe University

Ana Başlıklar: Sensor Technology | Colloid and Surface Chemistry

Dergi: Hacettepe Journal of Biology and Chemistry Vol.52 Issue 6, pp.405-414

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001319452

pISSN: 2687-475X

17-alpha-ethinylestradiol

Molecularly Imprinted Polymers

Surface Plasmon Resonance

Quartz Crystal Microbalance

Mass Sensor

Sayı

Yazarlar:

Meltem Okan | Memed Duman

Yayıncı:

Hacettepe University

Ana Başlıklar:

Sensor Technology | Colloid and Surface Chemistry

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.52 Issue 6, pp.405-414

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319452

pISSN:

2687-475X