Baculoviral vector loaded mesenchymal stem cells as efficient gene therapy tools for cancer treatment

SEDEF HANDE AKTAŞ; HAKAN AKBULUT; AYŞE ESER ELÇİN; MAHMUT PARMAKSIZ; ARZU KESKİN AKTAN; ARZU ÇÖLERİ CİHAN; YAŞAR MURAT ELÇİN; FİKRİ İÇLİ

Makale detayları

Baculoviral vector loaded mesenchymal stem cells as efficient gene therapy tools for cancer treatment

Yazarlar:

SEDEF HANDE AKTAŞ | HAKAN AKBULUT | AYŞE ESER ELÇİN | MAHMUT PARMAKSIZ | ARZU KESKİN AKTAN | ARZU ÇÖLERİ CİHAN | YAŞAR MURAT ELÇİN | FİKRİ İÇLİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 5, pp.1121-1128

Belge Tipi:

Makale

Yayın Tarihi:

9/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811593

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Insufficient targeting of the therapeutic genes to tumor cells is one of the major reasons for failure in cancer gene therapy. Mesenchymal stem cells (MSCs) seem to be a good candidate as a carrier for gene therapy because of its selective tumor tissue-homing properties. In the current study, we constructed baculoviral vectors (BVs) carrying cytosine deaminase (CD) (BV-CD) or green fluorescence protein (GFP) genes (BV-GFP) and tested the transduction efficiency of the vectors in tumor and mesenchymal stem cells. We also tested the in vivo efficacy of the BV-CD vector in a colon cancer model. Our results showed that the recombinant baculoviral vectors can efficiently transduce mammalian cells and express genes of interest. The BV-CD vector treatment caused significant in vitro cytotoxicity when used with 5-fluorocytosine. MSCs loaded with the BV-CD vector caused a significant delay in tumor growth and increased survival when compared to control and MSC alone treated groups bearing colon cancer.

Makale detayları

Baculoviral vector loaded mesenchymal stem cells as efficient gene therapy tools for cancer treatment

Yazarlar: SEDEF HANDE AKTAŞ | HAKAN AKBULUT | AYŞE ESER ELÇİN | MAHMUT PARMAKSIZ | ARZU KESKİN AKTAN | ARZU ÇÖLERİ CİHAN | YAŞAR MURAT ELÇİN | FİKRİ İÇLİ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 5, pp.1121-1128

Belge Tipi: Makale

Yayın Tarihi: 9/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000811593

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

SEDEF HANDE AKTAŞ | HAKAN AKBULUT | AYŞE ESER ELÇİN | MAHMUT PARMAKSIZ | ARZU KESKİN AKTAN | ARZU ÇÖLERİ CİHAN | YAŞAR MURAT ELÇİN | FİKRİ İÇLİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 5, pp.1121-1128

Belge Tipi:

Makale

Yayın Tarihi:

9/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811593

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152