Effect of Salt Stress on Germination and Seedling Development in Perennial Ryegrass (Lolium perenne L.) Seeds

Özlem Önal Aşcı; Yeliz Kaşko Arıcı; Özbay Dede

Makale detayları

Effect of Salt Stress on Germination and Seedling Development in Perennial Ryegrass (Lolium perenne L.) Seeds

Yazarlar:

Özlem Önal Aşcı | Yeliz Kaşko Arıcı | Özbay Dede

Yayıncı:

Siirt University

Ana Başlıklar:

Çayır-Mera ve Yem Bitkileri

Dergi:

Türkiye Tarımsal Araştırmalar Dergisi Vol.12 Issue 2, pp.178-184

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001353703

pISSN:

2148-2306

Anahtar kelimeler:

Özet:

 The study was carried out according to the randomized plot design in the petri dish in the laboratory of Ordu University Faculty of Agriculture in order to determine the effects of different salt doses on germination and seedling development of perennial ryegrass Lolium perenne L. Prana variety. In the study, 0, 30, 60, 90, 120 and 150 mM sodium chloride NaCl were applied to the caryopsiss. The obtained data were analyzed according to the One Way ANOVA, Welch ANOVA or t-test. Also, probit analysis was performed to determine the lethal dose. As a result of the statistical analysis, it was determined that salt stress had significantly negative effect on seed germination and seedling development. Salt in the germination medium decreased the germination percentage, rate and uniformity, and delayed the beginning of germination in seeds, and extended the mean germination time. The first statistically significant negative effect of salt stress on germination and plumule development occurred at the 60 mM. However, it was determined that there was no germination in seeds after the 90 mM NaCl, and that after the 60 mM NaCl, development in seedlings was inhibited to such an extent that measurement could not be taken. In addition, as a result of the probit analysis performed on the germination percentage in the Prana variety, 34.733 mM NaCl was calculated as the LD50 dose. As a result of all these, it was determined that the Prana variety was sensitive to salt stress during the germination period and could not tolerate NaCl above 34.733 mM.

 The study was carried out according to the randomized plot design in the petri dish in the laboratory of Ordu University Faculty of Agriculture in order to determine the effects of different salt doses on germination and seedling development of perennial ryegrass Lolium perenne L. Prana variety. In the study, 0, 30, 60, 90, 120 and 150 mM sodium chloride NaCl were applied to the caryopsiss. The obtained data were analyzed according to the One Way ANOVA, Welch ANOVA or t-test. Also, probit analysis was performed to determine the lethal dose. As a result of the statistical analysis, it was determined that salt stress had significantly negative effect on seed germination and seedling development. Salt in the germination medium decreased the germination percentage, rate and uniformity, and delayed the beginning of germination in seeds, and extended the mean germination time. The first statistically significant negative effect of salt stress on germination and plumule development occurred at the 60 mM. However, it was determined that there was no germination in seeds after the 90 mM NaCl, and that after the 60 mM NaCl, development in seedlings was inhibited to such an extent that measurement could not be taken. In addition, as a result of the probit analysis performed on the germination percentage in the Prana variety, 34.733 mM NaCl was calculated as the LD50 dose. As a result of all these, it was determined that the Prana variety was sensitive to salt stress during the germination period and could not tolerate NaCl above 34.733 mM.

 The study was carried out according to the randomized plot design in the petri dish in the laboratory of Ordu University Faculty of Agriculture in order to determine the effects of different salt doses on germination and seedling development of perennial ryegrass Lolium perenne L. Prana variety. In the study, 0, 30, 60, 90, 120 and 150 mM sodium chloride NaCl were applied to the caryopsiss. The obtained data were analyzed according to the One Way ANOVA, Welch ANOVA or t-test. Also, probit analysis was performed to determine the lethal dose. As a result of the statistical analysis, it was determined that salt stress had significantly negative effect on seed germination and seedling development. Salt in the germination medium decreased the germination percentage, rate and uniformity, and delayed the beginning of germination in seeds, and extended the mean germination time. The first statistically significant negative effect of salt stress on germination and plumule development occurred at the 60 mM. However, it was determined that there was no germination in seeds after the 90 mM NaCl, and that after the 60 mM NaCl, development in seedlings was inhibited to such an extent that measurement could not be taken. In addition, as a result of the probit analysis performed on the germination percentage in the Prana variety, 34.733 mM NaCl was calculated as the LD50 dose. As a result of all these, it was determined that the Prana variety was sensitive to salt stress during the germination period and could not tolerate NaCl above 34.733 mM.

 Çalışma, çok yıllık çim Lolium perenne L.in Prana çeşidinde farklı tuz dozlarının çimlenme ve fide gelişimine etkisini belirlemek amacıyla, Ordu Üniversitesi Ziraat Fakültesi laboratuvarında Petri çalışması olarak, tesadüf parselleri deneme desenine göre yürütülmüştür. Araştırmada tohumlara 0, 30, 60, 90, 120 ve 150 mM sodyum klorür NaCl dozları uygulanmıştır. Elde edilen veriler, tek yönlü varyans analizi klasik ANOVA veya Welch ANOVA ya da t-testi ile analiz edilmiş; ayrıca, lethal dozun belirlenmesi için probit analizi yapılmıştır. İstatistik analiz sonucunda, tuz stresinin tohumlarda çimlenmeyi ve fide gelişimini önemli derecede olumsuz etkilediği belirlenmiştir. Çimlenme ortamında bulunan tuz, çimlenme oranı, hızı ve üniformitesini azaltmış, çimlenme başlangıcını geciktirmiş, ortalama çimlenme süresini uzatmıştır. Çimlenme ve plumula gelişimi üzerinde tuz stresinin istatistiki olarak ilk önemli olumsuz etkisi 60 mM dozunda gerçekleşmiştir. Bununla birlikte 90 mM NaCl dozundan sonra tohumlarda çimlenme olmadığı, 60 mM NaCl dozundan sonra ise fidelerde gelişimin ölçüm alınamayacak kadar inhibe edildiği belirlenmiştir. Ayrıca çimlenme oranına yapılan probit analizi sonucunda 34.733 mM NaCl LD50 dozu olarak hesaplanmıştır. Tüm bunların sonucunda Prana çeşidinin çimlenme döneminde tuz stresine hassas olduğu, 34.733 mM değerinin üzerinde bulunan sodyum klorürü tolere edemediği belirlenmiştir.

Makale detayları

Effect of Salt Stress on Germination and Seedling Development in Perennial Ryegrass (Lolium perenne L.) Seeds

Yazarlar:

Özlem Önal Aşcı | Yeliz Kaşko Arıcı | Özbay Dede

Yayıncı:

Siirt University

Ana Başlıklar:

Çayır-Mera ve Yem Bitkileri

Dergi:

Türkiye Tarımsal Araştırmalar Dergisi Vol.12 Issue 2, pp.178-184

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001353703

pISSN:

2148-2306

Seed

salt

lethal dose

probit

Lolium perenneTohum

tuz

lethal doz

probit

Lolium perenne

Sayı

Makale detayları

Yazarlar: Özlem Önal Aşcı | Yeliz Kaşko Arıcı | Özbay Dede

Yayıncı: Siirt University

Ana Başlıklar: Çayır-Mera ve Yem Bitkileri

Dergi: Türkiye Tarımsal Araştırmalar Dergisi Vol.12 Issue 2, pp.178-184

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001353703

pISSN: 2148-2306

Sayı

Yazarlar:

Özlem Önal Aşcı | Yeliz Kaşko Arıcı | Özbay Dede

Yayıncı:

Siirt University

Ana Başlıklar:

Çayır-Mera ve Yem Bitkileri

Dergi:

Türkiye Tarımsal Araştırmalar Dergisi Vol.12 Issue 2, pp.178-184

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001353703

pISSN:

2148-2306