Botanical Origin, Antibacterial, and Antioxidant Properties of Polyfloral Bee Pollen from Kars (Türkiye): An Analytical Study

Neslihan Mutlu; Gül Esma Akdoğan Karadağ; Salih Akpınar

Makale detayları

Botanical Origin, Antibacterial, and Antioxidant Properties of Polyfloral Bee Pollen from Kars (Türkiye): An Analytical Study

Yazarlar:

Neslihan Mutlu | Gül Esma Akdoğan Karadağ | Salih Akpınar

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Bitki Morfolojisi ve Anatomisi | Bakteriyoloji

Dergi:

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.29 Issue 3, pp.842-853

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001338115

pISSN:

1300-5413

Anahtar kelimeler:

Özet:

 The aim of the present study is to investigate the botanical origin, antibacterial and antioxidant activities, as well as the total phenolic content and total flavonoid content of polyfloral bee pollen from Kars in the north-eastern region of Turkey. The pollen load was found to contain ten taxa, including Cistaceae, Boraginaceae, Brassicaceae, Fabaceae, Papaveraceae, Asteraceae, Polygonaceae, Rosaceae, Lamiaceae, and Plantaginaceae. The minimal inhibitory concentration MIC values against tested microorganisms ranged from 2.5 to 5 mg mL-1. The Folin-Ciocalteu method was used to determine the total phenolic content, which was found to be 23.65 mg gallic acid equivalent GAE g-1. The TFC value in the bee pollen ethanolic extract was determined to be 14.56 mg quercetin equivalent QE g-1. The antioxidant capacity of the bee pollen ethanolic extract was evaluated using the 1,1Diphenyl2picrylhydrazyl DPPH, cupric ion reducing antioxidant capacity CUPRAC, and 2,2Azinobis3ethylbenzthiazoline6sulphonic acid ABTS methods, which yielded results of 16.18 mg Trolox equivalent TE g-1, 54.23 mg TE g-1, and 91.9 mg TE g-1, respectively. The fatty acid compositions were identified using gas chromatographymass spectrometry GCMS. The predominant fatty acid was found to be α-linolenic acid 20.46 , followed sequentially by linoleic acid 16.68 , palmitic acid 12.94 , and arachidonic acid 9.65 . This study provides a comprehensive assessment of the botanical diversity, antibacterial and antioxidant properties, and chemical composition of polyfloral bee pollen from Kars.

 The aim of the present study is to investigate the botanical origin, antibacterial and antioxidant activities, as well as the total phenolic content and total flavonoid content of polyfloral bee pollen from Kars in the north-eastern region of Turkey. The pollen load was found to contain ten taxa, including Cistaceae, Boraginaceae, Brassicaceae, Fabaceae, Papaveraceae, Asteraceae, Polygonaceae, Rosaceae, Lamiaceae, and Plantaginaceae. The minimal inhibitory concentration MIC values against tested microorganisms ranged from 2.5 to 5 mg mL-1. The Folin-Ciocalteu method was used to determine the total phenolic content, which was found to be 23.65 mg gallic acid equivalent GAE g-1. The TFC value in the bee pollen ethanolic extract was determined to be 14.56 mg quercetin equivalent QE g-1. The antioxidant capacity of the bee pollen ethanolic extract was evaluated using the 1,1Diphenyl2picrylhydrazyl DPPH, cupric ion reducing antioxidant capacity CUPRAC, and 2,2Azinobis3ethylbenzthiazoline6sulphonic acid ABTS methods, which yielded results of 16.18 mg Trolox equivalent TE g-1, 54.23 mg TE g-1, and 91.9 mg TE g-1, respectively. The fatty acid compositions were identified using gas chromatographymass spectrometry GCMS. The predominant fatty acid was found to be α-linolenic acid 20.46 , followed sequentially by linoleic acid 16.68 , palmitic acid 12.94 , and arachidonic acid 9.65 . This study provides a comprehensive assessment of the botanical diversity, antibacterial and antioxidant properties, and chemical composition of polyfloral bee pollen from Kars.

 The aim of the present study is to investigate the botanical origin, antibacterial and antioxidant activities, as well as the total phenolic content and total flavonoid content of polyfloral bee pollen from Kars in the north-eastern region of Turkey. The pollen load was found to contain ten taxa, including Cistaceae, Boraginaceae, Brassicaceae, Fabaceae, Papaveraceae, Asteraceae, Polygonaceae, Rosaceae, Lamiaceae, and Plantaginaceae. The minimal inhibitory concentration MIC values against tested microorganisms ranged from 2.5 to 5 mg mL-1. The Folin-Ciocalteu method was used to determine the total phenolic content, which was found to be 23.65 mg gallic acid equivalent GAE g-1. The TFC value in the bee pollen ethanolic extract was determined to be 14.56 mg quercetin equivalent QE g-1. The antioxidant capacity of the bee pollen ethanolic extract was evaluated using the 1,1Diphenyl2picrylhydrazyl DPPH, cupric ion reducing antioxidant capacity CUPRAC, and 2,2Azinobis3ethylbenzthiazoline6sulphonic acid ABTS methods, which yielded results of 16.18 mg Trolox equivalent TE g-1, 54.23 mg TE g-1, and 91.9 mg TE g-1, respectively. The fatty acid compositions were identified using gas chromatographymass spectrometry GCMS. The predominant fatty acid was found to be α-linolenic acid 20.46 , followed sequentially by linoleic acid 16.68 , palmitic acid 12.94 , and arachidonic acid 9.65 . This study provides a comprehensive assessment of the botanical diversity, antibacterial and antioxidant properties, and chemical composition of polyfloral bee pollen from Kars.

 Bu çalışmanın amacı, Türkiyenin kuzey-doğusunda yer alan Karsta bulunan polifloral arı poleninin otanik kökeni, antibakteriyel ve antioksidan aktivitelerinin yanı sıra toplam fenolik içeriği ve toplam flavonoid içeriğini araştırmaktır. Polenin, Cistaceae, Boraginaceae, Brassicaceae, Fabaceae, Papaveraceae, Asteraceae, Polygonaceae, Rosaceae, Lamiaceae ve Plantaginaceae dahil olmak üzere on takson içerdiği bulundu. Test edilen mikroorganizmalara karşı minimum inhibitör konsantrasyon MİK değerleri 2.5 ile 5 mgmL arasında değişmektedir. Toplam fenolik içeriğin belirlenmesinde Folin-Ciocalteu yöntemi kullanılmış ve bu miktarın 23.65 mg gallik asit eşdeğeri GAEg olduğu belirlenmiştir. Arı poleni etanolik ekstraktındaki toplam flavonoid içerik değeri 14.56 mg kuersetin eşdeğeri KEg olarak belirlenmiştir. Arı poleni etanolik ekstraktının antioksidan kapasitesi, 1,1-Difenil-2-pikrilhidrazil DPPH, bakır iyon azaltıcı antioksidan kapasitesi CUPRAC ve 2,2-Azino-bis-3-etilbenztiyazolin-6- sülfonik asit ABTS yöntemleri kullanılarak değerlendirilmiş, sırasıyla 16.18 mg Trolox eşdeğeri TEg, 54. mg TEg ve 91.9 mg TEg sonuçları elde edilmiştir. Yağ asidi bileşimleri, gaz kromatografisikütle spektrometrisi GCMS kullanılarak belirlenmiştir. Baskın yağ asidinin α -linolenik asit 20.46 olduğu, bunu sırasıyla linoleik asit 16.68, palmitik asit 12.94 ve araşidonik asitin 9.65 takip ettiği tespit edilmiştir. Bu çalışmada, Karstan temin edilen polifloral arı poleninin botanik çeşitliliği, antibakteriyel ve antioksidan özellikleri ile kimyasal içeriği hakkında kapsamlı bir değerlendirme sunmaktadır.

Makale detayları

Botanical Origin, Antibacterial, and Antioxidant Properties of Polyfloral Bee Pollen from Kars (Türkiye): An Analytical Study

Yazarlar:

Neslihan Mutlu | Gül Esma Akdoğan Karadağ | Salih Akpınar

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Bitki Morfolojisi ve Anatomisi | Bakteriyoloji

Dergi:

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.29 Issue 3, pp.842-853

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001338115

pISSN:

1300-5413

Antibacterial activity

Antioxidant activity

Bee pollen

Fatty acid

GC/MSAntibakteriyel aktivite

Antioksidan aktivite

Arı poleni

GC/MS

Yağ asidi

Sayı

Makale detayları

Yazarlar: Neslihan Mutlu | Gül Esma Akdoğan Karadağ | Salih Akpınar

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Bitki Morfolojisi ve Anatomisi | Bakteriyoloji

Dergi: Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.29 Issue 3, pp.842-853

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001338115

pISSN: 1300-5413

Sayı

Yazarlar:

Neslihan Mutlu | Gül Esma Akdoğan Karadağ | Salih Akpınar

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Bitki Morfolojisi ve Anatomisi | Bakteriyoloji

Dergi:

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.29 Issue 3, pp.842-853

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001338115

pISSN:

1300-5413