Validation of Peracetic Acid as a Sporicide for Sterilization of Working Surfaces in Biological Safety Cabinets

Sandra R.B.R. Sella; Belquis P. Guizelini; Hindy Ribeiro

doi:https://doi.org/10.5799/ahinjs.02.2012.03.0051

Makale detayları

Validation of Peracetic Acid as a Sporicide for Sterilization of Working Surfaces in Biological Safety Cabinets

Yazarlar:

Sandra R.B.R. Sella | Belquis P. Guizelini | Hindy Ribeiro

Yayıncı:

Sağlık Araştırmaları Derneği

Dergi:

Journal of Microbiology and Infectious Diseases 2012 Vol.2 Issue 03, pp.93-99

Belge Tipi:

Makale

Yayın Tarihi:

8/2012

Belge Dili:

İngilizce

Document ID (Org No.):

2000488001

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/ahinjs.02.2012.03.0051

Özet:

 

Objectives: This study aimed to validate the use of peracetic acid as sporicide agent to decontaminate the working surface of a laminar flow biological safety cabinet (BSC), as an alternative to glutaraldehyde, including the selection of the disinfecting agent, the method of application, and the contact time.    	Materials and methods: The test organism was the spore-forming bacterium Bacillus atrophaeus ATCC 9372, which is a surrogate for an important infectious agent. Spore cultures were prepared from B. atrophaeus and used them to test the sporicidal efficacy of peracetic acid on a BSC stainless steel surface. The performance of the sterilant was assessed by determining minimal inhibitory concentration (MIC) and by microbial challenge in conditions that mimicked surface contamination. It was used 2.0% glutaraldehyde solution as the control.    	Results: The range of MICs was 0.6-1.1% for the control and 0.003–0.006% for the 0.2% peracetic acid solution. The 0.2% peracetic acid was an effective sterilant against B. atrophaeus spores (6-7 log spores, under defined conditions of use) after 40 min contact time, which was double that recommended on the product label.    	Conclusion: It was conclude that while the results of official methods can help to evaluate how products will perform, they are not usually reproducible in real-life user conditions and environments. Validation tests must be carried out to ensure the efficacy and safety of surface decontamination procedures.

Makale detayları

Validation of Peracetic Acid as a Sporicide for Sterilization of Working Surfaces in Biological Safety Cabinets

Yazarlar: Sandra R.B.R. Sella | Belquis P. Guizelini | Hindy Ribeiro

Yayıncı: Sağlık Araştırmaları Derneği

Dergi: Journal of Microbiology and Infectious Diseases 2012 Vol.2 Issue 03, pp.93-99

Belge Tipi: Makale

Yayın Tarihi: 8/2012

Belge Dili: İngilizce

Document ID (Org No.): 2000488001

Sıklık: Üç Aylık

eISSN: 2146-9369

pISSN: 2146-3158

DOI: https://doi.org/10.5799/ahinjs.02.2012.03.0051

Sayı

Yazarlar:

Sandra R.B.R. Sella | Belquis P. Guizelini | Hindy Ribeiro

Yayıncı:

Sağlık Araştırmaları Derneği

Dergi:

Journal of Microbiology and Infectious Diseases 2012 Vol.2 Issue 03, pp.93-99

Belge Tipi:

Makale

Yayın Tarihi:

8/2012

Belge Dili:

İngilizce

Document ID (Org No.):

2000488001

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/ahinjs.02.2012.03.0051