The presence of antibiotic resistance and molecular characterization of aminoglycoside and PmrA genes among food- and clinical-acquired Acinetobacter isolates

Mevhibe Terkuran; Zerrin Erginkaya; Fatih Köksal

Makale detayları

The presence of antibiotic resistance and molecular characterization of aminoglycoside and PmrA genes among food- and clinical-acquired Acinetobacter isolates

Yazarlar:

Mevhibe Terkuran | Zerrin Erginkaya | Fatih Köksal

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Food Hygiene and Technology | Veterinary Microbiology

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 2, pp.183-194

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311975

pISSN:

1300-0861

Anahtar kelimeler:

Özet:

 The assessment of antibiotic resistance and related genes of foodborne Acinetobacter spp. and the analysis of whether they are genetically related to clinical infection-agent strains are crucial in terms of sustainability of food safety. The study at hand investigated antibiotic resistance, aminoglycoside-modifying enzyme AME, and colistin resistance PmrA genes, clonal relationships while evaluating a possible correlation between antibiotic resistance and related genes between 27 foodborne and 50 clinical Acinetobacter spp. in Turkey. Antimicrobial susceptibilities, AME, PmrA genes, and clonal relatedness of the strains were performed by disc diffusion, PCR, and Pulsed Field gel Electrophoresis PFGE methods, respectively. The aph-AI, aph-6, anth3-I, aadA1, aadB, and PmrA genes were found as 48n24, 22n11, 14n7, 2n1, 4n2, and 92n46 respectively, in clinical strains. This rate was found as 51.9n14,59.3n16, 70.4n19, 7.4n2, 0n0, and 100n27, respectively in foodborne isolates. A positive correlation existed between the number of aph-AI gene positivity and trimethoprim-sulfamethoxazole and gentamycin resistance; anth 3-I gene positivity, and colistin resistance; PmrA gene positivity and piperacillin-tazobactam, ceftazidime, meropenem, amikacin, and imipenem resistance in clinical strains P0.05. A positive correlation between trimethoprim-sulfamethoxazole resistance and aadAI gene positivity was found in foodborne strains P0.05. Clonal relations were absent between foodborne and clinical A. baumanni species. Finally, AME genes rise parallel to multidrug-resistance in the clinical isolates, and foods may be potential reservoirs for disseminating multi-AME and PmrA genes while being susceptible to several antibiotics.

 The assessment of antibiotic resistance and related genes of foodborne Acinetobacter spp. and the analysis of whether they are genetically related to clinical infection-agent strains are crucial in terms of sustainability of food safety. The study at hand investigated antibiotic resistance, aminoglycoside-modifying enzyme AME, and colistin resistance PmrA genes, clonal relationships while evaluating a possible correlation between antibiotic resistance and related genes between 27 foodborne and 50 clinical Acinetobacter spp. in Turkey. Antimicrobial susceptibilities, AME, PmrA genes, and clonal relatedness of the strains were performed by disc diffusion, PCR, and Pulsed Field gel Electrophoresis PFGE methods, respectively. The aph-AI, aph-6, anth3-I, aadA1, aadB, and PmrA genes were found as 48n24, 22n11, 14n7, 2n1, 4n2, and 92n46 respectively, in clinical strains. This rate was found as 51.9n14,59.3n16, 70.4n19, 7.4n2, 0n0, and 100n27, respectively in foodborne isolates. A positive correlation existed between the number of aph-AI gene positivity and trimethoprim-sulfamethoxazole and gentamycin resistance; anth 3-I gene positivity, and colistin resistance; PmrA gene positivity and piperacillin-tazobactam, ceftazidime, meropenem, amikacin, and imipenem resistance in clinical strains P0.05. A positive correlation between trimethoprim-sulfamethoxazole resistance and aadAI gene positivity was found in foodborne strains P0.05. Clonal relations were absent between foodborne and clinical A. baumanni species. Finally, AME genes rise parallel to multidrug-resistance in the clinical isolates, and foods may be potential reservoirs for disseminating multi-AME and PmrA genes while being susceptible to several antibiotics.

 The assessment of antibiotic resistance and related genes of foodborne Acinetobacter spp. and the analysis of whether they are genetically related to clinical infection-agent strains are crucial in terms of sustainability of food safety. The study at hand investigated antibiotic resistance, aminoglycoside-modifying enzyme AME, and colistin resistance PmrA genes, clonal relationships while evaluating a possible correlation between antibiotic resistance and related genes between 27 foodborne and 50 clinical Acinetobacter spp. in Turkey. Antimicrobial susceptibilities, AME, PmrA genes, and clonal relatedness of the strains were performed by disc diffusion, PCR, and Pulsed Field gel Electrophoresis PFGE methods, respectively. The aph-AI, aph-6, anth3-I, aadA1, aadB, and PmrA genes were found as 48n24, 22n11, 14n7, 2n1, 4n2, and 92n46 respectively, in clinical strains. This rate was found as 51.9n14,59.3n16, 70.4n19, 7.4n2, 0n0, and 100n27, respectively in foodborne isolates. A positive correlation existed between the number of aph-AI gene positivity and trimethoprim-sulfamethoxazole and gentamycin resistance; anth 3-I gene positivity, and colistin resistance; PmrA gene positivity and piperacillin-tazobactam, ceftazidime, meropenem, amikacin, and imipenem resistance in clinical strains P0.05. A positive correlation between trimethoprim-sulfamethoxazole resistance and aadAI gene positivity was found in foodborne strains P0.05. Clonal relations were absent between foodborne and clinical A. baumanni species. Finally, AME genes rise parallel to multidrug-resistance in the clinical isolates, and foods may be potential reservoirs for disseminating multi-AME and PmrA genes while being susceptible to several antibiotics.

 The assessment of antibiotic resistance and related genes of foodborne Acinetobacter spp. and the analysis of whether they are genetically related to clinical infection-agent strains are crucial in terms of sustainability of food safety. The study at hand investigated antibiotic resistance, aminoglycoside-modifying enzyme AME, and colistin resistance PmrA genes, clonal relationships while evaluating a possible correlation between antibiotic resistance and related genes between 27 foodborne and 50 clinical Acinetobacter spp. in Turkey. Antimicrobial susceptibilities, AME, PmrA genes, and clonal relatedness of the strains were performed by disc diffusion, PCR, and Pulsed Field gel Electrophoresis PFGE methods, respectively. The aph-AI, aph-6, anth3-I, aadA1, aadB, and PmrA genes were found as 48n24, 22n11, 14n7, 2n1, 4n2, and 92n46 respectively, in clinical strains. This rate was found as 51.9n14,59.3n16, 70.4n19, 7.4n2, 0n0, and 100n27, respectively in foodborne isolates. A positive correlation existed between the number of aph-AI gene positivity and trimethoprim-sulfamethoxazole and gentamycin resistance; anth 3-I gene positivity, and colistin resistance; PmrA gene positivity and piperacillin-tazobactam, ceftazidime, meropenem, amikacin, and imipenem resistance in clinical strains P0.05. A positive correlation between trimethoprim-sulfamethoxazole resistance and aadAI gene positivity was found in foodborne strains P0.05. Clonal relations were absent between foodborne and clinical A. baumanni species. Finally, AME genes rise parallel to multidrug-resistance in the clinical isolates, and foods may be potential reservoirs for disseminating multi-AME and PmrA genes while being susceptible to several antibiotics.

Makale detayları

The presence of antibiotic resistance and molecular characterization of aminoglycoside and PmrA genes among food- and clinical-acquired Acinetobacter isolates

Yazarlar:

Mevhibe Terkuran | Zerrin Erginkaya | Fatih Köksal

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Food Hygiene and Technology | Veterinary Microbiology

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 2, pp.183-194

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311975

pISSN:

1300-0861

Acinetobacter spp.

AME genes

Dissemination

Foodborne

Sayı

Makale detayları

The presence of antibiotic resistance and molecular characterization of aminoglycoside and PmrA genes among food- and clinical-acquired Acinetobacter isolates

Yazarlar: Mevhibe Terkuran | Zerrin Erginkaya | Fatih Köksal

Yayıncı: Ankara University

Ana Başlıklar: Veterinary Food Hygiene and Technology | Veterinary Microbiology

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 2, pp.183-194

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001311975

pISSN: 1300-0861

Acinetobacter spp.

AME genes

Dissemination

Foodborne

Sayı

Yazarlar:

Mevhibe Terkuran | Zerrin Erginkaya | Fatih Köksal

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Food Hygiene and Technology | Veterinary Microbiology

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 2, pp.183-194

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311975

pISSN:

1300-0861