Effects of boric acid on oxidant-antioxidant, proinflammatory cytokine levels, and biochemical parameters in aged rats

Mehmet Başeğmez; Muhammed Fatih Doğan

Makale detayları

Effects of boric acid on oxidant-antioxidant, proinflammatory cytokine levels, and biochemical parameters in aged rats

Yazarlar:

Mehmet Başeğmez | Muhammed Fatih Doğan

Yayıncı:

Pamukkale University

Ana Başlıklar:

Tıbbi Fizyoloji (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.17 Issue 2, pp.369-379

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001300686

pISSN:

1308-0865

Anahtar kelimeler:

Özet:

 Purpose: As a result of the literature studies, it was seen that boric acid was the subject of many studies, and its effects on living things were investigated and examined. This study aimed to investigate the effects of oral boric acid supplementation at pharmacologic doses on physiological and biochemical systems in aged rats. Material and methods: A total of 32 Wistar Albino male-aged rats were randomly and equally divided into the following four groups: 1st; Control1 ml saline; 2nd; Low-dose boric acid L-BA10 mgkg; 3rd; Medium-dose boric acid M-BA20 mgkg; 4th; High-dose boric acid H-BA40 mgkg. Boric acid was given orally to aged rats for 28 days. Blood, liver, and kidney samples of rats were collected on day 29 to be analyzed for oxidants, antioxidants, proinflammatory cytokines, and biochemical changes. Result: Boric acid significantly increased albumin, total protein, calcium levels equally in all boric acid groups compared to the control group p0.05, increased cholesterol parameter only in H-BA group p0.05, increased phosphor level in M-BA and H-BA groups compared to control and L-BA groups p0.05, total bilirubin level was increased only in L-BA group p0.05, blood urea nitrogen level was increased in L-BA and M-BA groups p0.05, alanine aminotransferase level was increased only in M-BA group p0.05, creatine kinase and glucose levels were increased boric acid in all groups compared to control group p0.05. However, boric acid did not affect globulin, creatine, alkaline phosphatase, and amylase levels in a dose-dependent manner p0.05. Boric acid significantly decreased MDA levels p0.05 and increased GSH, SOD, and CAT enzyme activities p0.05 in liver and kidney tissues in a dose-dependent manner. In addition, boric acid decreased plasma IL-6 and TNF-α proinflammatory cytokine levels p0.05. Conclusion: This study demonstrated that boric acid supplementation has ameliorative effects in a dose-dependent manner on lipid peroxidation, immunomodulation, and regulation of many blood biochemical parameters in aged rats.

 Purpose: As a result of the literature studies, it was seen that boric acid was the subject of many studies, and its effects on living things were investigated and examined. This study aimed to investigate the effects of oral boric acid supplementation at pharmacologic doses on physiological and biochemical systems in aged rats. Material and methods: A total of 32 Wistar Albino male-aged rats were randomly and equally divided into the following four groups: 1st; Control1 ml saline; 2nd; Low-dose boric acid L-BA10 mgkg; 3rd; Medium-dose boric acid M-BA20 mgkg; 4th; High-dose boric acid H-BA40 mgkg. Boric acid was given orally to aged rats for 28 days. Blood, liver, and kidney samples of rats were collected on day 29 to be analyzed for oxidants, antioxidants, proinflammatory cytokines, and biochemical changes. Result: Boric acid significantly increased albumin, total protein, calcium levels equally in all boric acid groups compared to the control group p0.05, increased cholesterol parameter only in H-BA group p0.05, increased phosphor level in M-BA and H-BA groups compared to control and L-BA groups p0.05, total bilirubin level was increased only in L-BA group p0.05, blood urea nitrogen level was increased in L-BA and M-BA groups p0.05, alanine aminotransferase level was increased only in M-BA group p0.05, creatine kinase and glucose levels were increased boric acid in all groups compared to control group p0.05. However, boric acid did not affect globulin, creatine, alkaline phosphatase, and amylase levels in a dose-dependent manner p0.05. Boric acid significantly decreased MDA levels p0.05 and increased GSH, SOD, and CAT enzyme activities p0.05 in liver and kidney tissues in a dose-dependent manner. In addition, boric acid decreased plasma IL-6 and TNF-α proinflammatory cytokine levels p0.05. Conclusion: This study demonstrated that boric acid supplementation has ameliorative effects in a dose-dependent manner on lipid peroxidation, immunomodulation, and regulation of many blood biochemical parameters in aged rats.

 Purpose: As a result of the literature studies, it was seen that boric acid was the subject of many studies, and its effects on living things were investigated and examined. This study aimed to investigate the effects of oral boric acid supplementation at pharmacologic doses on physiological and biochemical systems in aged rats. Material and methods: A total of 32 Wistar Albino male-aged rats were randomly and equally divided into the following four groups: 1st; Control1 ml saline; 2nd; Low-dose boric acid L-BA10 mgkg; 3rd; Medium-dose boric acid M-BA20 mgkg; 4th; High-dose boric acid H-BA40 mgkg. Boric acid was given orally to aged rats for 28 days. Blood, liver, and kidney samples of rats were collected on day 29 to be analyzed for oxidants, antioxidants, proinflammatory cytokines, and biochemical changes. Result: Boric acid significantly increased albumin, total protein, calcium levels equally in all boric acid groups compared to the control group p0.05, increased cholesterol parameter only in H-BA group p0.05, increased phosphor level in M-BA and H-BA groups compared to control and L-BA groups p0.05, total bilirubin level was increased only in L-BA group p0.05, blood urea nitrogen level was increased in L-BA and M-BA groups p0.05, alanine aminotransferase level was increased only in M-BA group p0.05, creatine kinase and glucose levels were increased boric acid in all groups compared to control group p0.05. However, boric acid did not affect globulin, creatine, alkaline phosphatase, and amylase levels in a dose-dependent manner p0.05. Boric acid significantly decreased MDA levels p0.05 and increased GSH, SOD, and CAT enzyme activities p0.05 in liver and kidney tissues in a dose-dependent manner. In addition, boric acid decreased plasma IL-6 and TNF-α proinflammatory cytokine levels p0.05. Conclusion: This study demonstrated that boric acid supplementation has ameliorative effects in a dose-dependent manner on lipid peroxidation, immunomodulation, and regulation of many blood biochemical parameters in aged rats.

 Amaç: Yapılan literatür çalışmaları neticesinde borik asit in birçok çalışmaya konu olduğu ve canlıya olan etkilerinin araştırılarak incelendiği goru lmus tu r. Bu çalışmada yaşlı sıçanlarda farmakolojik dozlarda uygulanan oral borik asit takviyesinin fizyolojik ve biyokimyasal sistemlerdeki etkilerinin araştırılması amaçlanmıştır. Gereç ve yöntem: Toplam 32 adet Wistar Albino erkek yaşlı sıçan rastgele ve eşit olarak aşağıdaki dört gruba ayrılmıştır: 1.; Kontrol1ml salin, 2.; Düşük-doz borik asit L-BA10 mgkg, 3.; Orta-doz borik asit M-BA20 mgkg, 4.; Yüksek-doz borik asit H-BA40 mgkg. Yaşlı sıçanlara 28 gün boyunca borik asit oral olarak verilmiştir. Sıçanların kanı, karaciğer ve böbrek örnekleri oksidan, antioksidan, proinflamatuar sitokinler ve biyokimyasal değişiklikler açısından analiz edilmek üzere 29. günde toplanmıştır. Bulgular: Borik asit, albümin, total protein ve kalsiyum düzeylerini kontrol grubuna kıyasla tüm borik asit gruplarında eşit oranda artırırken p0,05, kolesterol parametresini sadece H-BA grubunda artırmıştır p0,05. Borik asit fosfor düzeyini kontrol ve L-BA gruplarına kıyasla M-BA ve H-BA gruplarında artırırken p0,05, total bilirubin düzeyini sadece L-BA grubunda p0,05, kan üre nitrojen düzeyini L-BA ve M-BA gruplarında artırmıştır p0,05. Ayrıca alanin aminotransferaz düzeyini sadece M-BA grubunda artırırken p0,05, kreatin kinaz ve glukoz düzeylerini kontrol grubuna kıyasla tüm gruplarda artırdığı tespit edilmiştir p0,05. Ancak borik asidin globulin, kreatin, alkalin fosfataz, amilaz düzeyleri üzerinde doza bağlı bir etkisi olmamıştır p0,05. Borik asit karaciğer ve böbrek dokularında MDA düzeylerini önemli ölçüde azaltmış p0,05 ve GSH, SOD ve CAT enzim aktivitelerini artırmıştır p0,05. Ayrıca, borik asit plazma IL-6 ve TNF-α proinflamatuar sitokin düzeylerini azaltmıştır p0,05.

Makale detayları

Effects of boric acid on oxidant-antioxidant, proinflammatory cytokine levels, and biochemical parameters in aged rats

Yazarlar:

Mehmet Başeğmez | Muhammed Fatih Doğan

Yayıncı:

Pamukkale University

Ana Başlıklar:

Tıbbi Fizyoloji (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.17 Issue 2, pp.369-379

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001300686

pISSN:

1308-0865

Age

antioxidant

boric acid

oxidant

proinflammatory cytokineYaş

antioksidan

borik asit

oksidan

proinflamatuar sitokin

Sayı

Makale detayları

Yazarlar: Mehmet Başeğmez | Muhammed Fatih Doğan

Yayıncı: Pamukkale University

Ana Başlıklar: Tıbbi Fizyoloji (Diğer)

Dergi: Pamukkale Tıp Dergisi Vol.17 Issue 2, pp.369-379

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001300686

pISSN: 1308-0865

Sayı

Yazarlar:

Mehmet Başeğmez | Muhammed Fatih Doğan

Yayıncı:

Pamukkale University

Ana Başlıklar:

Tıbbi Fizyoloji (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.17 Issue 2, pp.369-379

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001300686

pISSN:

1308-0865