Protective effects of methylprednisolone in kidney: aortic occlusion-reperfusion model in rats

Serkan Seçici; Kadir Kaan Özsin; M.özgür Özyiğit; Omer Arda; Yasemin Üstündağ

Makale detayları

Protective effects of methylprednisolone in kidney: aortic occlusion-reperfusion model in rats

Yazarlar:

Serkan Seçici | Kadir Kaan Özsin | M.özgür Özyiğit | Omer Arda | Yasemin Üstündağ

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Cardiovascular Surgery

Dergi:

The European Research Journal Vol.9 Issue 3, pp.567-573

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284936

pISSN:

2149-3189

Anahtar kelimeler:

Özet:

 Objectives: Ischemiareperfusion IR injury is commonly seen in cardiovascular surgery, activates inflammation and causes renal damage. In this experimental study, we aimed to assess the effects of different doses 5 and 30 mgkg of methylprednisolone MP, which has anti-inflammatory effect, on renal ischemiareperfusion IR injury. Methods: Thirty-two male Wistar albino rats were randomly divided into four groups n  8. The sham group underwent midline laparotomy and dissection of the abdominal aorta without occlusion while the IR group underwent suprarenal aortic ischemia for 45 minutes followed by 180 minutes of reperfusion. In the 5 mgkg MP and 30 mgkg MP groups, MP was administered intraperitoneally. At the end of the experiment, blood samples were obtained, and kidneys were extracted. Results: Pretreatment with methylprednisolone did not influence serum BUN and creatinine levels. Serum TNF-α levels and ischemia-modified albumin levels were significantly lower in the MP groups compared to the IR group  p  0.05. Histological examination demonstrated severe injury in the IR group and treatment with MP attenuated the severity. The difference was significant in doses of 30 mgkg MP. Conclusions: This results of the model of renal IR injury presented in this work reveal the anti-inflammatory and the protective effects of MP in cases of renal IR.

 Objectives: Ischemiareperfusion IR injury is commonly seen in cardiovascular surgery, activates inflammation and causes renal damage. In this experimental study, we aimed to assess the effects of different doses 5 and 30 mgkg of methylprednisolone MP, which has anti-inflammatory effect, on renal ischemiareperfusion IR injury. Methods: Thirty-two male Wistar albino rats were randomly divided into four groups n  8. The sham group underwent midline laparotomy and dissection of the abdominal aorta without occlusion while the IR group underwent suprarenal aortic ischemia for 45 minutes followed by 180 minutes of reperfusion. In the 5 mgkg MP and 30 mgkg MP groups, MP was administered intraperitoneally. At the end of the experiment, blood samples were obtained, and kidneys were extracted. Results: Pretreatment with methylprednisolone did not influence serum BUN and creatinine levels. Serum TNF-α levels and ischemia-modified albumin levels were significantly lower in the MP groups compared to the IR group  p  0.05. Histological examination demonstrated severe injury in the IR group and treatment with MP attenuated the severity. The difference was significant in doses of 30 mgkg MP. Conclusions: This results of the model of renal IR injury presented in this work reveal the anti-inflammatory and the protective effects of MP in cases of renal IR.

 Objectives: Ischemiareperfusion IR injury is commonly seen in cardiovascular surgery, activates inflammation and causes renal damage. In this experimental study, we aimed to assess the effects of different doses 5 and 30 mgkg of methylprednisolone MP, which has anti-inflammatory effect, on renal ischemiareperfusion IR injury. Methods: Thirty-two male Wistar albino rats were randomly divided into four groups n  8. The sham group underwent midline laparotomy and dissection of the abdominal aorta without occlusion while the IR group underwent suprarenal aortic ischemia for 45 minutes followed by 180 minutes of reperfusion. In the 5 mgkg MP and 30 mgkg MP groups, MP was administered intraperitoneally. At the end of the experiment, blood samples were obtained, and kidneys were extracted. Results: Pretreatment with methylprednisolone did not influence serum BUN and creatinine levels. Serum TNF-α levels and ischemia-modified albumin levels were significantly lower in the MP groups compared to the IR group  p  0.05. Histological examination demonstrated severe injury in the IR group and treatment with MP attenuated the severity. The difference was significant in doses of 30 mgkg MP. Conclusions: This results of the model of renal IR injury presented in this work reveal the anti-inflammatory and the protective effects of MP in cases of renal IR.

 Objectives: Ischemiareperfusion IR injury is commonly seen in cardiovascular surgery, activates inflammation and causes renal damage. In this experimental study, we aimed to assess the effects of different doses 5 and 30 mgkg of methylprednisolone MP, which has anti-inflammatory effect, on renal ischemiareperfusion IR injury. Methods: Thirty-two male Wistar albino rats were randomly divided into four groups n  8. The sham group underwent midline laparotomy and dissection of the abdominal aorta without occlusion while the IR group underwent suprarenal aortic ischemia for 45 minutes followed by 180 minutes of reperfusion. In the 5 mgkg MP and 30 mgkg MP groups, MP was administered intraperitoneally. At the end of the experiment, blood samples were obtained, and kidneys were extracted. Results: Pretreatment with methylprednisolone did not influence serum BUN and creatinine levels. Serum TNF-α levels and ischemia-modified albumin levels were significantly lower in the MP groups compared to the IR group  p  0.05. Histological examination demonstrated severe injury in the IR group and treatment with MP attenuated the severity. The difference was significant in doses of 30 mgkg MP. Conclusions: This results of the model of renal IR injury presented in this work reveal the anti-inflammatory and the protective effects of MP in cases of renal IR.

Makale detayları

Protective effects of methylprednisolone in kidney: aortic occlusion-reperfusion model in rats

Yazarlar:

Serkan Seçici | Kadir Kaan Özsin | M.özgür Özyiğit | Omer Arda | Yasemin Üstündağ

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Cardiovascular Surgery

Dergi:

The European Research Journal Vol.9 Issue 3, pp.567-573

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284936

pISSN:

2149-3189

Ischemia-reperfusion injury

kidney

methylprednisolone

inflammation

Sayı

Makale detayları

Protective effects of methylprednisolone in kidney: aortic occlusion-reperfusion model in rats

Yazarlar: Serkan Seçici | Kadir Kaan Özsin | M.özgür Özyiğit | Omer Arda | Yasemin Üstündağ

Yayıncı: Prusa Medical Publishing

Ana Başlıklar: Cardiovascular Surgery

Dergi: The European Research Journal Vol.9 Issue 3, pp.567-573

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001284936

pISSN: 2149-3189

Ischemia-reperfusion injury

kidney

methylprednisolone

inflammation

Sayı

Yazarlar:

Serkan Seçici | Kadir Kaan Özsin | M.özgür Özyiğit | Omer Arda | Yasemin Üstündağ

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Cardiovascular Surgery

Dergi:

The European Research Journal Vol.9 Issue 3, pp.567-573

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284936

pISSN:

2149-3189