Developing and Validation of a High-Performance Liquid Chromatography Method for the Determination of Combined Perindopril, Indapamide and Amlodipine from Pharmaceutical Preparations

Sevinç Ayla Özsar; Sacide Altınöz

Makale detayları

Developing and Validation of a High-Performance Liquid Chromatography Method for the Determination of Combined Perindopril, Indapamide and Amlodipine from Pharmaceutical Preparations

Yazarlar:

Sevinç Ayla Özsar | Sacide Altınöz

Yayıncı:

Hacettepe University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry

Dergi:

Hacettepe University Journal of the Faculty of Pharmacy Vol.44 Issue 4, pp.306-317

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001332478

pISSN:

2458-8806

Anahtar kelimeler:

Özet:

 High Performance Liquid Chromatographic method was developed and validated for the analysis of a pharmaceutical preparation TRIPLIXAM consist of Perindopril PRN, İndapamide IND and Amlodipine AML which are used for the control of hypertension in patients with high blood pressure. In this developed HPLC method, ACE 5 C18 analytical column 12.5 x 4.6 mm was used for the analysis of PRN, IND and AML. Acetonitril and 50 mM phosphate buffer mixture 40:60 vv was used as mobile phase. Measurements were obtained at 215 nm wavelength using UV detector. Retention times of PRN, AML and IND were 2.22, 3.48 and 5.05 min. respectively, at selected chromatographical conditions. The developed HPLC method was validated in terms of stability, accuracy, sensitivity, linearity, precision, specificity, ruggedness and robustness in accordance with ICH analytical method. Linear calibration curves were obtained in the range of 1.0-25.0 ppm; 1.0-8.0 ppm and 1.0-40.0 ppm for PRN, IND and AML, respectively. In this HPLC method the limit of dedection values LOD for PRN, IND and AML were 0.25 ppm, 0.10 ppm and 0.15 ppm while limit of quantification values LOQ were 0.50 ppm, 0.25 ppm and 0.40 ppm, respectively.

 High Performance Liquid Chromatographic method was developed and validated for the analysis of a pharmaceutical preparation TRIPLIXAM consist of Perindopril PRN, İndapamide IND and Amlodipine AML which are used for the control of hypertension in patients with high blood pressure. In this developed HPLC method, ACE 5 C18 analytical column 12.5 x 4.6 mm was used for the analysis of PRN, IND and AML. Acetonitril and 50 mM phosphate buffer mixture 40:60 vv was used as mobile phase. Measurements were obtained at 215 nm wavelength using UV detector. Retention times of PRN, AML and IND were 2.22, 3.48 and 5.05 min. respectively, at selected chromatographical conditions. The developed HPLC method was validated in terms of stability, accuracy, sensitivity, linearity, precision, specificity, ruggedness and robustness in accordance with ICH analytical method. Linear calibration curves were obtained in the range of 1.0-25.0 ppm; 1.0-8.0 ppm and 1.0-40.0 ppm for PRN, IND and AML, respectively. In this HPLC method the limit of dedection values LOD for PRN, IND and AML were 0.25 ppm, 0.10 ppm and 0.15 ppm while limit of quantification values LOQ were 0.50 ppm, 0.25 ppm and 0.40 ppm, respectively.

 High Performance Liquid Chromatographic method was developed and validated for the analysis of a pharmaceutical preparation TRIPLIXAM consist of Perindopril PRN, İndapamide IND and Amlodipine AML which are used for the control of hypertension in patients with high blood pressure. In this developed HPLC method, ACE 5 C18 analytical column 12.5 x 4.6 mm was used for the analysis of PRN, IND and AML. Acetonitril and 50 mM phosphate buffer mixture 40:60 vv was used as mobile phase. Measurements were obtained at 215 nm wavelength using UV detector. Retention times of PRN, AML and IND were 2.22, 3.48 and 5.05 min. respectively, at selected chromatographical conditions. The developed HPLC method was validated in terms of stability, accuracy, sensitivity, linearity, precision, specificity, ruggedness and robustness in accordance with ICH analytical method. Linear calibration curves were obtained in the range of 1.0-25.0 ppm; 1.0-8.0 ppm and 1.0-40.0 ppm for PRN, IND and AML, respectively. In this HPLC method the limit of dedection values LOD for PRN, IND and AML were 0.25 ppm, 0.10 ppm and 0.15 ppm while limit of quantification values LOQ were 0.50 ppm, 0.25 ppm and 0.40 ppm, respectively.

 High Performance Liquid Chromatographic method was developed and validated for the analysis of a pharmaceutical preparation TRIPLIXAM consist of Perindopril PRN, İndapamide IND and Amlodipine AML which are used for the control of hypertension in patients with high blood pressure. In this developed HPLC method, ACE 5 C18 analytical column 12.5 x 4.6 mm was used for the analysis of PRN, IND and AML. Acetonitril and 50 mM phosphate buffer mixture 40:60 vv was used as mobile phase. Measurements were obtained at 215 nm wavelength using UV detector. Retention times of PRN, AML and IND were 2.22, 3.48 and 5.05 min. respectively, at selected chromatographical conditions. The developed HPLC method was validated in terms of stability, accuracy, sensitivity, linearity, precision, specificity, ruggedness and robustness in accordance with ICH analytical method. Linear calibration curves were obtained in the range of 1.0-25.0 ppm; 1.0-8.0 ppm and 1.0-40.0 ppm for PRN, IND and AML, respectively. In this HPLC method the limit of dedection values LOD for PRN, IND and AML were 0.25 ppm, 0.10 ppm and 0.15 ppm while limit of quantification values LOQ were 0.50 ppm, 0.25 ppm and 0.40 ppm, respectively.

Makale detayları

Developing and Validation of a High-Performance Liquid Chromatography Method for the Determination of Combined Perindopril, Indapamide and Amlodipine from Pharmaceutical Preparations

Yazarlar:

Sevinç Ayla Özsar | Sacide Altınöz

Yayıncı:

Hacettepe University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry

Dergi:

Hacettepe University Journal of the Faculty of Pharmacy Vol.44 Issue 4, pp.306-317

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001332478

pISSN:

2458-8806

Amlodipine

High Performance Liquid Chromatography

Indapamide

Perindopril

Validation

Sayı

Makale detayları

Developing and Validation of a High-Performance Liquid Chromatography Method for the Determination of Combined Perindopril, Indapamide and Amlodipine from Pharmaceutical Preparations

Yazarlar: Sevinç Ayla Özsar | Sacide Altınöz

Yayıncı: Hacettepe University

Ana Başlıklar: Pharmaceutical Analytical Chemistry

Dergi: Hacettepe University Journal of the Faculty of Pharmacy Vol.44 Issue 4, pp.306-317

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001332478

pISSN: 2458-8806

Amlodipine

High Performance Liquid Chromatography

Indapamide

Perindopril

Validation

Sayı

Yazarlar:

Sevinç Ayla Özsar | Sacide Altınöz

Yayıncı:

Hacettepe University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry

Dergi:

Hacettepe University Journal of the Faculty of Pharmacy Vol.44 Issue 4, pp.306-317

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001332478

pISSN:

2458-8806