Protective Effects of the Purinergic Receptor Antagonist A438079 on Lipopolysaccharide-Induced Kidney Injury in Rats

Seçkin Özkanlar

Makale detayları

Protective Effects of the Purinergic Receptor Antagonist A438079 on Lipopolysaccharide-Induced Kidney Injury in Rats

Yazarlar:

Seçkin Özkanlar

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Bilimleri

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.3 Issue 1, pp.6-11

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287429

pISSN:

2791-8645

Anahtar kelimeler:

Özet:

 Sepsis is an important clinical problem associated with organ dysfunction and high mortality. To investigate the effects of this purinergic receptor on renal dysfunction in sepsis, a rat model was created by the intraperitoneal injection of lipopolysaccharides. The groups were control, A438079, lipopolysaccharides, and lipopolysaccharideA438079. Following lipopolysaccharide 8 mgkg injection, A438079 15 mgkg was administrated intraperitoneally in the study groups. Then, blood and kidney tissues were collected for analysis from rats sacrificed under anesthesia. In the histopathological evaluations, it was observed that A438079 reduced the damage caused by sepsis. It was observed that the creatinine and blood urea nitrogen concentrations of the serum samples belonging to the lipopolysaccharideA438079 group were lower than the serum samples belonging to the lipopolysaccharide group. Aquaporin-2, Bcl-2, caspase-3, and NfkB-p65 protein expressions in kidney tissues were evaluated by western blot. On the other hand, it was observed that there was a decrease in the expression of these proteins in the A438079-treated groups. In conclusion, A438079 can reduce the inflammatory response in acute kidney injury induced by lipopolysaccharides and improve renal dysfunction.

 Sepsis is an important clinical problem associated with organ dysfunction and high mortality. To investigate the effects of this purinergic receptor on renal dysfunction in sepsis, a rat model was created by the intraperitoneal injection of lipopolysaccharides. The groups were control, A438079, lipopolysaccharides, and lipopolysaccharideA438079. Following lipopolysaccharide 8 mgkg injection, A438079 15 mgkg was administrated intraperitoneally in the study groups. Then, blood and kidney tissues were collected for analysis from rats sacrificed under anesthesia. In the histopathological evaluations, it was observed that A438079 reduced the damage caused by sepsis. It was observed that the creatinine and blood urea nitrogen concentrations of the serum samples belonging to the lipopolysaccharideA438079 group were lower than the serum samples belonging to the lipopolysaccharide group. Aquaporin-2, Bcl-2, caspase-3, and NfkB-p65 protein expressions in kidney tissues were evaluated by western blot. On the other hand, it was observed that there was a decrease in the expression of these proteins in the A438079-treated groups. In conclusion, A438079 can reduce the inflammatory response in acute kidney injury induced by lipopolysaccharides and improve renal dysfunction.

 Sepsis is an important clinical problem associated with organ dysfunction and high mortality. To investigate the effects of this purinergic receptor on renal dysfunction in sepsis, a rat model was created by the intraperitoneal injection of lipopolysaccharides. The groups were control, A438079, lipopolysaccharides, and lipopolysaccharideA438079. Following lipopolysaccharide 8 mgkg injection, A438079 15 mgkg was administrated intraperitoneally in the study groups. Then, blood and kidney tissues were collected for analysis from rats sacrificed under anesthesia. In the histopathological evaluations, it was observed that A438079 reduced the damage caused by sepsis. It was observed that the creatinine and blood urea nitrogen concentrations of the serum samples belonging to the lipopolysaccharideA438079 group were lower than the serum samples belonging to the lipopolysaccharide group. Aquaporin-2, Bcl-2, caspase-3, and NfkB-p65 protein expressions in kidney tissues were evaluated by western blot. On the other hand, it was observed that there was a decrease in the expression of these proteins in the A438079-treated groups. In conclusion, A438079 can reduce the inflammatory response in acute kidney injury induced by lipopolysaccharides and improve renal dysfunction.

 Sepsis, organ disfonksiyonu ve yüksek mortalite ile ilişkili önemli bir klinik problemdir. Bu pürinerjik reseptörün sepsiste böbrek fonksiyon bozukluğu üzerindeki etkisini araştırmak için intraperitoneal LPS Lipopolisakarit enjeksiyonu ile sıçan modeli oluşturuldu. Çalışma Kontrol, A438079, LPS, LPS ve LPSA438079 gruplarından oluşmaktaydı. Çalışma gruplarına LPS 8 mgkg enjeksiyonunu takiben A438079 15 mgkg uygulandı i.p. Daha sonra anestezi altında sakrifiye edilen ratlardan analizler için kan ve böbrek dokuları toplandı. Histopatolojik değerlendirmelerde A438079un sepsisin neden olduğu hasarı azalttığı görüldü. LPSA438079 grubuna ait serum örneklerinin kreatinin ve BUN Blood Urea Nitrogen konsantrasyonlarının LPS grubuna ait serum örneklerine göre daha düşük olduğu belirlendi. Böbrek dokularında AQP2, Bcl-2, Kaspaz-3 ve NfkB-p65 protein ekspresyonları western blot ile değerlendirildi. Öte yandan, A438079 ile tedavi edilen gruplarda bu proteinlerin ifadesinde azalma olduğu gözlendi. Sonuç olarak, A438079, LPSnin neden olduğu akut böbrek hasarında enflamatuar yanıtı azaltabilir ve böbrek fonksiyon bozukluğunu iyileştirebileceği belirlenmiştir.

Makale detayları

Protective Effects of the Purinergic Receptor Antagonist A438079 on Lipopolysaccharide-Induced Kidney Injury in Rats

Yazarlar:

Seçkin Özkanlar

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Bilimleri

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.3 Issue 1, pp.6-11

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287429

pISSN:

2791-8645

A438079

LPS

pürinerjik reseptör

sıçanlar

sepsis

A438079

LPS

purinergic receptor

rats

sepsis

Sayı

Makale detayları

Yazarlar: Seçkin Özkanlar

Yayıncı: Ataturk University

Ana Başlıklar: Veteriner Bilimleri

Dergi: Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.3 Issue 1, pp.6-11

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001287429

pISSN: 2791-8645

Sayı

Yazarlar:

Seçkin Özkanlar

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Bilimleri

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.3 Issue 1, pp.6-11

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287429

pISSN:

2791-8645