INVESTIGATION OF DICER1 AND BAFF GENE MUTATIONS IN B-CELL NON-HODGKIN LYMPHOMA

Nurcan Cirak; Demet Akdeniz; Hülya Yazıcı

Makale detayları

INVESTIGATION OF DICER1 AND BAFF GENE MUTATIONS IN B-CELL NON-HODGKIN LYMPHOMA

Yazarlar:

Nurcan Cirak | Demet Akdeniz | Hülya Yazıcı

Yayıncı:

Istanbul University

Ana Başlıklar:

Medical Genetics (Excl. Cancer Genetics)

Dergi:

Journal of Advanced Research in Health Sciences Vol.7 Issue 1, pp.8-16

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319135

pISSN:

2651-4060

Anahtar kelimeler:

Özet:

 Objective: DICER1 and BAFF gene mutations are effective in T-cell lymphoma progression. Therefore, DICER1 and BAFF genes may have a role in the progression of B-cell lymphomas. For this reason, it was aimed to determine the role of DICER1 and BAFF genes in the development of B-NHL. Materials and Methods: The study included DNA samples from 60 patients diagnosed with B-NHL who had applied to the Istanbul University,Institute of Oncology, Department of Clinical Oncology between 1991 and 1997. DNA materials obtained from lymphocytes of 30 healthy individuals matched with the patients in terms of age, gender, and race were used as a control group. The c.3473AG rs3742330 polymorphism in the DICER1 gene and the c.-871CT rs9514828 single nucleotide polymorphism in the BAFF gene were examined using the PCR-RFLP method. In addition, the presence of mutations in the 11th and 25th exons of the DICER1 gene was evaluated by SANGER sequencing. The results of the control and patient groups were analyzed for polymorphism and mutation presence using Chi-square and Fisher tests. Results: The polymorphic regions of c.3473AGrs3742330 in the DICER1 gene and c.-871CT rs 9514828 in the BAFF gene were examined in the patient and control groups, but no statistically significant relationship was found. When the exon 11 and exon 25 of the DICER1 gene were investigated, no statistically significant relationship was found between the patient and control groupsp0.05. Conclusion: The absence of a difference between the patient and control groups suggests that different genetic mechanisms may be involved in the formation of B-NHL. The small population in the study is one of the reasons why no significant difference was found between the results. Additional studies are needed in larger patient and control groups.

 Objective: DICER1 and BAFF gene mutations are effective in T-cell lymphoma progression. Therefore, DICER1 and BAFF genes may have a role in the progression of B-cell lymphomas. For this reason, it was aimed to determine the role of DICER1 and BAFF genes in the development of B-NHL. Materials and Methods: The study included DNA samples from 60 patients diagnosed with B-NHL who had applied to the Istanbul University,Institute of Oncology, Department of Clinical Oncology between 1991 and 1997. DNA materials obtained from lymphocytes of 30 healthy individuals matched with the patients in terms of age, gender, and race were used as a control group. The c.3473AG rs3742330 polymorphism in the DICER1 gene and the c.-871CT rs9514828 single nucleotide polymorphism in the BAFF gene were examined using the PCR-RFLP method. In addition, the presence of mutations in the 11th and 25th exons of the DICER1 gene was evaluated by SANGER sequencing. The results of the control and patient groups were analyzed for polymorphism and mutation presence using Chi-square and Fisher tests. Results: The polymorphic regions of c.3473AGrs3742330 in the DICER1 gene and c.-871CT rs 9514828 in the BAFF gene were examined in the patient and control groups, but no statistically significant relationship was found. When the exon 11 and exon 25 of the DICER1 gene were investigated, no statistically significant relationship was found between the patient and control groupsp0.05. Conclusion: The absence of a difference between the patient and control groups suggests that different genetic mechanisms may be involved in the formation of B-NHL. The small population in the study is one of the reasons why no significant difference was found between the results. Additional studies are needed in larger patient and control groups.

 Objective: DICER1 and BAFF gene mutations are effective in T-cell lymphoma progression. Therefore, DICER1 and BAFF genes may have a role in the progression of B-cell lymphomas. For this reason, it was aimed to determine the role of DICER1 and BAFF genes in the development of B-NHL. Materials and Methods: The study included DNA samples from 60 patients diagnosed with B-NHL who had applied to the Istanbul University,Institute of Oncology, Department of Clinical Oncology between 1991 and 1997. DNA materials obtained from lymphocytes of 30 healthy individuals matched with the patients in terms of age, gender, and race were used as a control group. The c.3473AG rs3742330 polymorphism in the DICER1 gene and the c.-871CT rs9514828 single nucleotide polymorphism in the BAFF gene were examined using the PCR-RFLP method. In addition, the presence of mutations in the 11th and 25th exons of the DICER1 gene was evaluated by SANGER sequencing. The results of the control and patient groups were analyzed for polymorphism and mutation presence using Chi-square and Fisher tests. Results: The polymorphic regions of c.3473AGrs3742330 in the DICER1 gene and c.-871CT rs 9514828 in the BAFF gene were examined in the patient and control groups, but no statistically significant relationship was found. When the exon 11 and exon 25 of the DICER1 gene were investigated, no statistically significant relationship was found between the patient and control groupsp0.05. Conclusion: The absence of a difference between the patient and control groups suggests that different genetic mechanisms may be involved in the formation of B-NHL. The small population in the study is one of the reasons why no significant difference was found between the results. Additional studies are needed in larger patient and control groups.

 Amaç: DICER1 ve BAFF geni mutasyonlarının T hücreli lenfoma progresyonunda etkili olduğu bilinmektedir. Bu durum B hücreli lenfomaların progresyonunda da DICER1 ve BAFF genlerinin rolünün olabileceğini düşündürmüştür. DICER1 ve BAFF geninin ekspresyon ve mutasyonlarını araştıran birçok çalışma bulunmasına rağmen, B hücreli non-Hodgkin lenfoma progresyonunda tümör baskılayıcı etkisinin nasıl oluştuğu ile ilgili olarak yeterli bilgi bulunmamaktadır. Bu sebeple çalışmada, DICER1 ve BAFF genlerinin B-NHL gelişimindeki rolünün ne olduğunun belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmaya 1991-1997 yılları arasında İstanbul Üniversitesi, Onkoloji Enstitüsü, Klinik Onkoloji Anabilim Dalına başvurmuş ve B-NHL tanısı almış 60 hastaya ait DNA örnekleri dâhil edilmiştir. Kontrol grubu olarak hastalarla yaş, cinsiyet ve ırk olarak eşleştirilmiş 30 sağlıklı kişinin lenfositlerinden elde edilen DNA materyalleri kullanılmıştır. DICER1 geninde yer alan c.3473AGrs3742330 polimorfizmi ile BAFF genindeki c.-871CTrs9514828 tek nükleotid polimorfizmi PCR-RFLP yöntemiyle incelenmiştir. Ayrıca DICER1 geninin 11. ve 25. ekzonları mutasyon varlığı açısından SANGER dizi analizi yöntemiyle değerlendirilmiştir. Kontrol ve hasta grubunun sonuçları polimorfizm ve mutasyon varlığı açısından Ki-kare ve Fisher testleriyle analiz edilmiştir. Bulgular: DICER1 genindeki c.3473AGrs3742330 ile BAFF genindeki c.-871CTrs 9514828 polimorfik bölgeleri hasta ve kontrol grubunda incelenmiş ancak istatistiksel olarak anlamlı bir ilişki bulunamamıştır. DICER1 geninin 11. ve 25. Ekzonları araştırıldığında ise yine hasta ve kontrol grubu arasında istatiksel olarak anlamlı bir ilişki bulunamamıştır p0,05. Sonuç: Hasta ve kontrol grupları arasında bir farkın bulunmaması, B-NHL oluşumunda farklı genetik mekanizmaların etkisinin olabileceğini düşündürmektedir. Çalışmadaki popülasyon sayısının az olması, sonuçlar arasında anlamlı bir fark bulunamamasının nedenlerinden biridir. Daha geniş hasta ve kontrol grubunda ek çalışmalara ihtiyaç vardır.

Makale detayları

INVESTIGATION OF DICER1 AND BAFF GENE MUTATIONS IN B-CELL NON-HODGKIN LYMPHOMA

Yazarlar:

Nurcan Cirak | Demet Akdeniz | Hülya Yazıcı

Yayıncı:

Istanbul University

Ana Başlıklar:

Medical Genetics (Excl. Cancer Genetics)

Dergi:

Journal of Advanced Research in Health Sciences Vol.7 Issue 1, pp.8-16

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319135

pISSN:

2651-4060

B-cell non-Hodgkin lymphoma

DICER1

BAFF

mutation

SNPB hücreli non-Hodgkin lenfoma

DICER1

BAFF

mutasyon

SNP

Sayı

Makale detayları

Yazarlar: Nurcan Cirak | Demet Akdeniz | Hülya Yazıcı

Yayıncı: Istanbul University

Ana Başlıklar: Medical Genetics (Excl. Cancer Genetics)

Dergi: Journal of Advanced Research in Health Sciences Vol.7 Issue 1, pp.8-16

Belge Tipi: Makale

Yayın Tarihi: 2/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001319135

pISSN: 2651-4060

Sayı

Yazarlar:

Nurcan Cirak | Demet Akdeniz | Hülya Yazıcı

Yayıncı:

Istanbul University

Ana Başlıklar:

Medical Genetics (Excl. Cancer Genetics)

Dergi:

Journal of Advanced Research in Health Sciences Vol.7 Issue 1, pp.8-16

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319135

pISSN:

2651-4060