Comparative evaluation of indirect enzyme linked immunosorbent assay, rose bengal plate test, microagglutination test, and polymerase chain reaction for diagnosis of brucellosis in buffaloes

Malik RAIES UL ISLAM; Mohinder PRATAP GUPTA; Pritam KAUR SIDHU; Gursimran FILIA; Hari MOHAN SAXENA; Tawheed AHMED SHAFI

doi:https://doi.org/10.3906/sag-1210-84

Makale detayları

Comparative evaluation of indirect enzyme linked immunosorbent assay, rose bengal plate test, microagglutination test, and polymerase chain reaction for diagnosis of brucellosis in buffaloes

Yazarlar:

Malik RAIES UL ISLAM | Mohinder PRATAP GUPTA | Pritam KAUR SIDHU | Gursimran FILIA | Hari MOHAN SAXENA | Tawheed AHMED SHAFI

Yayıncı:

TUBITAK

Ana Başlıklar:

Hayvancılık

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 3, pp.306-310

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000766454

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

DOI:

https://doi.org/10.3906/sag-1210-84

Anahtar kelimeler:

Özet:

 

In the present study, 178 blood samples from buffaloes were tested against indirect enzyme linked immunosorbent assay (I-ELISA), rose bengal plate test (RBPT), microagglutination test (MAT), modified microagglutination test (mMAT), and polymerase chain reaction (PCR) to select the most suitable test for efficient and effective diagnosis of bovine brucellosis. Win Episcope 2 software was used to determine the agreement between tests (kappa values at 95% confidence interval). I-ELISA was pair compared with all the other tests. Out of 178 samples, 102 were found positive by I-ELISA, 81 by RBPT, 85 by MAT, 79 by mMAT, and 68 by PCR. Substantial agreement was observed between I-ELISA and RBPT (k = 0.72), I-ELISA and MAT ((k = 0.65), and I-ELISA and mMAT ((k = 0.67). The least degree of agreement was observed between I-ELISA and PCR ((k = 0.15). I-ELISA detected more samples as positive among these tests. The results of the present study indicate that I-ELISA can be used for routine sero-diagnosis of Brucella infection in buffaloes. Furthermore, PCR can be used in combination with I-ELISA to complement the serological diagnosis, especially in the initial phase when the immune response of the animal is not detectable.

 

In the present study, 178 blood samples from buffaloes were tested against indirect enzyme linked immunosorbent assay (I-ELISA), rose bengal plate test (RBPT), microagglutination test (MAT), modified microagglutination test (mMAT), and polymerase chain reaction (PCR) to select the most suitable test for efficient and effective diagnosis of bovine brucellosis. Win Episcope 2 software was used to determine the agreement between tests (kappa values at 95% confidence interval). I-ELISA was pair compared with all the other tests. Out of 178 samples, 102 were found positive by I-ELISA, 81 by RBPT, 85 by MAT, 79 by mMAT, and 68 by PCR. Substantial agreement was observed between I-ELISA and RBPT (k = 0.72), I-ELISA and MAT ((k = 0.65), and I-ELISA and mMAT ((k = 0.67). The least degree of agreement was observed between I-ELISA and PCR ((k = 0.15). I-ELISA detected more samples as positive among these tests. The results of the present study indicate that I-ELISA can be used for routine sero-diagnosis of Brucella infection in buffaloes. Furthermore, PCR can be used in combination with I-ELISA to complement the serological diagnosis, especially in the initial phase when the immune response of the animal is not detectable.

Makale detayları

Comparative evaluation of indirect enzyme linked immunosorbent assay, rose bengal plate test, microagglutination test, and polymerase chain reaction for diagnosis of brucellosis in buffaloes

Yazarlar:

Malik RAIES UL ISLAM | Mohinder PRATAP GUPTA | Pritam KAUR SIDHU | Gursimran FILIA | Hari MOHAN SAXENA | Tawheed AHMED SHAFI

Yayıncı:

TUBITAK

Ana Başlıklar:

Hayvancılık

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 3, pp.306-310

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000766454

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

DOI:

https://doi.org/10.3906/sag-1210-84

Key words: Brucellosis

I-ELISA

RBPT

MAT

mMAT

PCR

Sayı

Makale detayları

Comparative evaluation of indirect enzyme linked immunosorbent assay, rose bengal plate test, microagglutination test, and polymerase chain reaction for diagnosis of brucellosis in buffaloes

Yazarlar: Malik RAIES UL ISLAM | Mohinder PRATAP GUPTA | Pritam KAUR SIDHU | Gursimran FILIA | Hari MOHAN SAXENA | Tawheed AHMED SHAFI

Yayıncı: TUBITAK

Ana Başlıklar: Hayvancılık

Dergi: Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 3, pp.306-310

Belge Tipi: Makale

Yayın Tarihi: 2/2013

Belge Dili: İngilizce

Document ID (Org No.): 2000766454

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

DOI: https://doi.org/10.3906/sag-1210-84

Key words: Brucellosis

I-ELISA

RBPT

MAT

mMAT

PCR

Sayı

Yazarlar:

Malik RAIES UL ISLAM | Mohinder PRATAP GUPTA | Pritam KAUR SIDHU | Gursimran FILIA | Hari MOHAN SAXENA | Tawheed AHMED SHAFI

Yayıncı:

TUBITAK

Ana Başlıklar:

Hayvancılık

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 3, pp.306-310

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000766454

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

DOI:

https://doi.org/10.3906/sag-1210-84