Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds

EMEL ÖZKAN ÜNAL; EDA ŞEYMA KEPENEK; HAVVA DİNÇ; FÜSUN ÖZER; GİZEM SÖNMEZ; İNCİ ZEHRA TOĞAN; MEHMET İHSAN SOYSAL

Makale detayları

Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds

Yazarlar:

EMEL ÖZKAN ÜNAL | EDA ŞEYMA KEPENEK | HAVVA DİNÇ | FÜSUN ÖZER | GİZEM SÖNMEZ | İNCİ ZEHRA TOĞAN | MEHMET İHSAN SOYSAL

Yayıncı:

TUBITAK

Ana Başlıklar:

Zooloji bilimleri/Hayvanlar

Dergi:

Turkish Journal of Zoology 2015 Vol.39 Issue 5, pp.734-748

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000774846

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179

Anahtar kelimeler:

Özet:

 

The aim of this study was to determine the genetic diversity of 4 native Turkish cattle breeds, based on the growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) genes. In order to study the polymorphisms in these genes, the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was performed. A 329-bp fragment and a 223-bp fragment of GH, a 156-bp fragment of PRL, and a 411-bp fragment of DGAT1, thus 4 loci of 3 genes, were amplified via PCR. These fragments were then restricted with the enzymes MspI, Alu1, RsaI, and CfrI, respectively. In this study, 2 types of alleles,  ( + ) and ( - ) for the GH-MspI, L and V for the GH-Alu1, A and B for the PRL, and K and A for the DGAT1 loci, were observed. The results of the present study will contribute to the polymorphism data on the world's cattle breeds. Furthermore, the above-mentioned allele frequencies of Turkish native breeds are evaluated in relation to their genetic relatedness, and to infer their milk production properties on the basis of the available literature. In turn, these results can be utilized for future breeding programs of dairy cattle in Turkey.

 

The aim of this study was to determine the genetic diversity of 4 native Turkish cattle breeds, based on the growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) genes. In order to study the polymorphisms in these genes, the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was performed. A 329-bp fragment and a 223-bp fragment of GH, a 156-bp fragment of PRL, and a 411-bp fragment of DGAT1, thus 4 loci of 3 genes, were amplified via PCR. These fragments were then restricted with the enzymes MspI, Alu1, RsaI, and CfrI, respectively. In this study, 2 types of alleles,  ( + ) and ( - ) for the GH-MspI, L and V for the GH-Alu1, A and B for the PRL, and K and A for the DGAT1 loci, were observed. The results of the present study will contribute to the polymorphism data on the world's cattle breeds. Furthermore, the above-mentioned allele frequencies of Turkish native breeds are evaluated in relation to their genetic relatedness, and to infer their milk production properties on the basis of the available literature. In turn, these results can be utilized for future breeding programs of dairy cattle in Turkey.

Makale detayları

Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds

Yazarlar:

EMEL ÖZKAN ÜNAL | EDA ŞEYMA KEPENEK | HAVVA DİNÇ | FÜSUN ÖZER | GİZEM SÖNMEZ | İNCİ ZEHRA TOĞAN | MEHMET İHSAN SOYSAL

Yayıncı:

TUBITAK

Ana Başlıklar:

Zooloji bilimleri/Hayvanlar

Dergi:

Turkish Journal of Zoology 2015 Vol.39 Issue 5, pp.734-748

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000774846

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179

Milk protein

PCR-RFLP

GH

PRL

DGAT1

Turkish native cattle breeds

Sayı

Makale detayları

Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds

Yazarlar: EMEL ÖZKAN ÜNAL | EDA ŞEYMA KEPENEK | HAVVA DİNÇ | FÜSUN ÖZER | GİZEM SÖNMEZ | İNCİ ZEHRA TOĞAN | MEHMET İHSAN SOYSAL

Yayıncı: TUBITAK

Ana Başlıklar: Zooloji bilimleri/Hayvanlar

Dergi: Turkish Journal of Zoology 2015 Vol.39 Issue 5, pp.734-748

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: İngilizce

Document ID (Org No.): 2000774846

Sıklık: İki Aylık

eISSN: 1303-6114

pISSN: 1300-0179

Milk protein

PCR-RFLP

GH

PRL

DGAT1

Turkish native cattle breeds

Sayı

Yazarlar:

EMEL ÖZKAN ÜNAL | EDA ŞEYMA KEPENEK | HAVVA DİNÇ | FÜSUN ÖZER | GİZEM SÖNMEZ | İNCİ ZEHRA TOĞAN | MEHMET İHSAN SOYSAL

Yayıncı:

TUBITAK

Ana Başlıklar:

Zooloji bilimleri/Hayvanlar

Dergi:

Turkish Journal of Zoology 2015 Vol.39 Issue 5, pp.734-748

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000774846

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179