ORIGANUM ONITES L. ETHANOLIC EXTRACT ALLEVIATES ACETIC ACID-INDUCED ULCERATIVE COLITIS IN RATS BY REGULATING OXIDANT, INFLAMMATORY, AND APOPTOTIC MARKERS

Dilek Özbeyli; Ali Şen; Leyla Bitiş; Naziye Özkan Yenal; Ayşe Nur Hazar Yavuz; Deniz Mukaddes Türet; Meral Yüksel; Ahmet Doğan; Aslı Aykaç

Makale detayları

ORIGANUM ONITES L. ETHANOLIC EXTRACT ALLEVIATES ACETIC ACID-INDUCED ULCERATIVE COLITIS IN RATS BY REGULATING OXIDANT, INFLAMMATORY, AND APOPTOTIC MARKERS

Yazarlar:

Dilek Özbeyli | Ali Şen | Leyla Bitiş | Naziye Özkan Yenal | Ayşe Nur Hazar Yavuz | Deniz Mukaddes Türet | Meral Yüksel | Ahmet Doğan | Aslı Aykaç

Yayıncı:

Ankara University

Ana Başlıklar:

Farmakognozi | Klinik Farmakoloji ve Terapötikler | Tıbbi Farmakoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.631-650

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356402

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: This study aims to evaluate the antioxidant and anti-inflammatory effects of Origanum onites ethanolic extract O. onites EE in vitro and its effects on inflammation, apoptosis, oxidative stress, and tissue damage-related parameters in the acetic acid AA-induced ulcerative colitis UC model in rats. Material and Method: Dried parts of O. onites were extracted with ethanol using the maceration method. Then, the extracts anti-DPPH and anti-ABTS free radical scavenging, anti-5-lipoxygenase LOX activities, and total secondary metabolic quantities were evaluated in vitro. The rat groups were determined as control  saline C, AA  saline A, AA  O. onites EE O, AA  Sulfasalazine S. The treatments were applied once a day for 3 consecutive days. Result and Discussion: O. onites EE showed high amount of total phenolic, total flavonoid, and total triterpene contents, high antioxidant activity against ABTS, DPPH radicals, and anti-inflammatory effects against 5-LOX enzyme. Due to AA-induction, increased luminol-lucigenin, TNF-α, IL-1β, IL-17, TLR-9, caspase-3, caspase-9, MMP-3, macroscopic and microscopic scores attenuated by O. onites EE and sulfasalazine treatments. In conclusion, O. onites EE has a significant therapeutic effect on rats colitis due to its total flavonoid, phenolic, and triterpene content, and its Anti-5-LOX and free radical scavenging activity.

 Objective: This study aims to evaluate the antioxidant and anti-inflammatory effects of Origanum onites ethanolic extract O. onites EE in vitro and its effects on inflammation, apoptosis, oxidative stress, and tissue damage-related parameters in the acetic acid AA-induced ulcerative colitis UC model in rats. Material and Method: Dried parts of O. onites were extracted with ethanol using the maceration method. Then, the extracts anti-DPPH and anti-ABTS free radical scavenging, anti-5-lipoxygenase LOX activities, and total secondary metabolic quantities were evaluated in vitro. The rat groups were determined as control  saline C, AA  saline A, AA  O. onites EE O, AA  Sulfasalazine S. The treatments were applied once a day for 3 consecutive days. Result and Discussion: O. onites EE showed high amount of total phenolic, total flavonoid, and total triterpene contents, high antioxidant activity against ABTS, DPPH radicals, and anti-inflammatory effects against 5-LOX enzyme. Due to AA-induction, increased luminol-lucigenin, TNF-α, IL-1β, IL-17, TLR-9, caspase-3, caspase-9, MMP-3, macroscopic and microscopic scores attenuated by O. onites EE and sulfasalazine treatments. In conclusion, O. onites EE has a significant therapeutic effect on rats colitis due to its total flavonoid, phenolic, and triterpene content, and its Anti-5-LOX and free radical scavenging activity.

 Objective: This study aims to evaluate the antioxidant and anti-inflammatory effects of Origanum onites ethanolic extract O. onites EE in vitro and its effects on inflammation, apoptosis, oxidative stress, and tissue damage-related parameters in the acetic acid AA-induced ulcerative colitis UC model in rats. Material and Method: Dried parts of O. onites were extracted with ethanol using the maceration method. Then, the extracts anti-DPPH and anti-ABTS free radical scavenging, anti-5-lipoxygenase LOX activities, and total secondary metabolic quantities were evaluated in vitro. The rat groups were determined as control  saline C, AA  saline A, AA  O. onites EE O, AA  Sulfasalazine S. The treatments were applied once a day for 3 consecutive days. Result and Discussion: O. onites EE showed high amount of total phenolic, total flavonoid, and total triterpene contents, high antioxidant activity against ABTS, DPPH radicals, and anti-inflammatory effects against 5-LOX enzyme. Due to AA-induction, increased luminol-lucigenin, TNF-α, IL-1β, IL-17, TLR-9, caspase-3, caspase-9, MMP-3, macroscopic and microscopic scores attenuated by O. onites EE and sulfasalazine treatments. In conclusion, O. onites EE has a significant therapeutic effect on rats colitis due to its total flavonoid, phenolic, and triterpene content, and its Anti-5-LOX and free radical scavenging activity.

 Amaç: Bu çalışmada Origanum onites etanolik özütünün O. onites EE antioksidan ve anti-inflamatuar etkileri in vitro olarak ve inflamasyon, apoptoz, oksidatif stres ve doku hasarıyla ilişkili parametreler üzerindeki etkileri asetik asit AA ile indüklenen sıçan ülseratif kolit UC modelinde değerlendirilmiştir. Gereç ve Yöntem: O. onitesin kurutulmuş kısımları maserasyon yöntemi kullanılarak etanol ile ekstre edildi. Daha sonra, ekstrenin anti-DPPH ve anti-ABTS serbest radikal temizleme, anti-5-lipoksijenaz aktiviteleri ve toplam sekonder metabolik miktarları ölçüldü. Sıçan grupları kontrol  salin C, UC  salin A, UC  O. onites EE O, UC  Sülfasalazin S olarak belirlendi. Tüm tedaviler 3 ardışık gün boyunca günde bir kez uygulandı. Sonuç ve Tartışma: O. onites EE, toplam fenolik, flavonoid ve triterpen içeriklerine iyi miktarda, sahipti ve ABTS, DPPH radikallerine karşı yüksek bir antioksidan aktivite ve 5-LOX enzimine karşı yüksek bir anti-inflamatuar aktivite gösterdi. AA ile uygulanması ile artan luminol-lusigenin, TNF-α, IL-1, IL-17, TLR-9, kaspaz-3, kaspaz-9, MMP-3, makroskobik ve mikroskobik skorlar, O. onites EE ve sülfasalazin tedavileriyle azaldı. Sonuç olarak, O. onites EEnin sıçanlarda kolit üzerinde iyi bir terapötik etkisi vardır; bu da esas olarak total flavonoid, fenolik ve triterpen içeriği ve Anti-5-LOX ve serbest radikal temizleyici aktivitesinden kaynaklanmaktadır.

Makale detayları

ORIGANUM ONITES L. ETHANOLIC EXTRACT ALLEVIATES ACETIC ACID-INDUCED ULCERATIVE COLITIS IN RATS BY REGULATING OXIDANT, INFLAMMATORY, AND APOPTOTIC MARKERS

Yazarlar:

Dilek Özbeyli | Ali Şen | Leyla Bitiş | Naziye Özkan Yenal | Ayşe Nur Hazar Yavuz | Deniz Mukaddes Türet | Meral Yüksel | Ahmet Doğan | Aslı Aykaç

Yayıncı:

Ankara University

Ana Başlıklar:

Farmakognozi | Klinik Farmakoloji ve Terapötikler | Tıbbi Farmakoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.631-650

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356402

pISSN:

1015-3918

Anti-5-LOX

apoptosis

IL-17

IL-10

Origanum onites

TLR-9

ulcerative colitisAnti-5-LOX

apoptoz

IL-17

IL-10

Origanum onites

TRL-9

ülseratif kolit

Sayı

Makale detayları

Yazarlar: Dilek Özbeyli | Ali Şen | Leyla Bitiş | Naziye Özkan Yenal | Ayşe Nur Hazar Yavuz | Deniz Mukaddes Türet | Meral Yüksel | Ahmet Doğan | Aslı Aykaç

Yayıncı: Ankara University

Ana Başlıklar: Farmakognozi | Klinik Farmakoloji ve Terapötikler | Tıbbi Farmakoloji

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.631-650

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001356402

pISSN: 1015-3918

Sayı

Yazarlar:

Dilek Özbeyli | Ali Şen | Leyla Bitiş | Naziye Özkan Yenal | Ayşe Nur Hazar Yavuz | Deniz Mukaddes Türet | Meral Yüksel | Ahmet Doğan | Aslı Aykaç

Yayıncı:

Ankara University

Ana Başlıklar:

Farmakognozi | Klinik Farmakoloji ve Terapötikler | Tıbbi Farmakoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.631-650

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356402

pISSN:

1015-3918