In VIVO MODULATORY EFFECT of MALACHITE GREEN on CYTOCHROME P450 AND ANTIOXIDANT ENZYME ACTIVITIES in RAINBOW TROUT LIVER

Azra Bozcaarmutlu Büken; Volkan Yıldırım; Canan Sapmaz; Ahmet Günbür; Hakan Türker

Makale detayları

In VIVO MODULATORY EFFECT of MALACHITE GREEN on CYTOCHROME P450 AND ANTIOXIDANT ENZYME ACTIVITIES in RAINBOW TROUT LIVER

Yazarlar:

Azra Bozcaarmutlu Büken | Volkan Yıldırım | Canan Sapmaz | Ahmet Günbür | Hakan Türker

Yayıncı:

Ataturk University

Ana Başlıklar:

Sucul Toksikoloji

Dergi:

The Trout Journal of Atatürk University Vol.3 Issue 1, pp.10-18

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353086

pISSN:

2980-2539

Anahtar kelimeler:

Özet:

 Malachite green is a mutagenic and carcinogenic chemical used as a dye in textile and paper industry and in fish farms against ectoparasites, bacterial and fungal infections. Aquatic organisms are at risk of malachite green exposure. In this study, our aim was to determine the effect of two different doses of malachite green on cytochrome P450 system and antioxidant system in rainbow trout Oncorhynchus mykiss. For this purpose, 24 fish were randomly divided into three groups: the control, the 0.1 mgL malachite green treatment group and the 0.5 mgL malachite green treatment group. Highly elevated 7-ethoxyresorufin-O-deethylase activities EROD were measured in fish treated with 0.5 mgL malachite green. 7-pentoxyresorufin O-depentylase PROD activities increased with increasing doses of malachite green. Catalase CAT and glutathione reductase GR activities in the 0.1 mgL malachite green administration group were higher than those in the control group. Erythromycin N-demethylase ERND and glutathione S-transferase GST activities were not significantly affected by malachite green treatment in either treatment group. The results of this study clearly demonstrate that malachite green has a modulatory effect on EROD, PROD, CAT, and GR activities in rainbow trout.

 Malachite green is a mutagenic and carcinogenic chemical used as a dye in textile and paper industry and in fish farms against ectoparasites, bacterial and fungal infections. Aquatic organisms are at risk of malachite green exposure. In this study, our aim was to determine the effect of two different doses of malachite green on cytochrome P450 system and antioxidant system in rainbow trout Oncorhynchus mykiss. For this purpose, 24 fish were randomly divided into three groups: the control, the 0.1 mgL malachite green treatment group and the 0.5 mgL malachite green treatment group. Highly elevated 7-ethoxyresorufin-O-deethylase activities EROD were measured in fish treated with 0.5 mgL malachite green. 7-pentoxyresorufin O-depentylase PROD activities increased with increasing doses of malachite green. Catalase CAT and glutathione reductase GR activities in the 0.1 mgL malachite green administration group were higher than those in the control group. Erythromycin N-demethylase ERND and glutathione S-transferase GST activities were not significantly affected by malachite green treatment in either treatment group. The results of this study clearly demonstrate that malachite green has a modulatory effect on EROD, PROD, CAT, and GR activities in rainbow trout.

 Malachite green is a mutagenic and carcinogenic chemical used as a dye in textile and paper industry and in fish farms against ectoparasites, bacterial and fungal infections. Aquatic organisms are at risk of malachite green exposure. In this study, our aim was to determine the effect of two different doses of malachite green on cytochrome P450 system and antioxidant system in rainbow trout Oncorhynchus mykiss. For this purpose, 24 fish were randomly divided into three groups: the control, the 0.1 mgL malachite green treatment group and the 0.5 mgL malachite green treatment group. Highly elevated 7-ethoxyresorufin-O-deethylase activities EROD were measured in fish treated with 0.5 mgL malachite green. 7-pentoxyresorufin O-depentylase PROD activities increased with increasing doses of malachite green. Catalase CAT and glutathione reductase GR activities in the 0.1 mgL malachite green administration group were higher than those in the control group. Erythromycin N-demethylase ERND and glutathione S-transferase GST activities were not significantly affected by malachite green treatment in either treatment group. The results of this study clearly demonstrate that malachite green has a modulatory effect on EROD, PROD, CAT, and GR activities in rainbow trout.

 Malahit yeşili, tekstil ve kağıt endüstrisinde boya olarak ve balık çiftliklerinde ektoparazitlere, bakteriyel ve fungal enfeksiyonlara karşı kullanılan mutajenik ve karsinojenik bir kimyasaldır. Sucul organizmalar malahit yeşiline maruz kalma riski altındadır. Bu çalışmada amacımız gökkuşağı alabalığında Oncorhynchus mykiss iki farklı konsantrasyonda malahit yeşilinin sitokrom P450 ve antioksidan sistem üzerine etkisini belirlemektir. Bu amaçla, 24 balık üç gruba rastgele ayrılmıştır: kontrol, 0,1 mgL malahit yeşili ile muamele edilen grup ve 0,5 mgL malahit yeşili ile muamele edilen grup. Oldukça yüksek 7-etoksiresorufin O-deetilaz EROD aktivitesi balıklarda 0,5 mgL malahit yeşili ile muamele edilen grupta ölçülmüştür. 7-pentoksiresorufin O-depentilaz PROD aktivitesi artan malahit yeşili dozu ile artmıştır. Katalaz CAT ve glutatyon redüktaz aktiviteleri 0,1 mgL malahit yeşili ile muamele edilen grupta kontrol grubundan yüksektir. Eritromisin N-demetilaz ERND ve glutatyon S-transferaz GST aktiviteleri her iki muamele grubunda malahit yeşilinden önemli ölçüde etkilenmemiştir. Bu çalışmanın sonuçları malahit yeşilinin gökkuşağı alabalığında EROD, PROD, CAT ve GR aktiviteleri üzerinde modülatör bir etkiye sahip olduğunu açıkça göstermektedir.

Makale detayları

In VIVO MODULATORY EFFECT of MALACHITE GREEN on CYTOCHROME P450 AND ANTIOXIDANT ENZYME ACTIVITIES in RAINBOW TROUT LIVER

Yazarlar:

Azra Bozcaarmutlu Büken | Volkan Yıldırım | Canan Sapmaz | Ahmet Günbür | Hakan Türker

Yayıncı:

Ataturk University

Ana Başlıklar:

Sucul Toksikoloji

Dergi:

The Trout Journal of Atatürk University Vol.3 Issue 1, pp.10-18

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353086

pISSN:

2980-2539

Antioxidant enzyme activities

Cytochrome P450

Malachite green

Rainbow trout (Oncorhynchus mykiss)

Xenobiotic metabolizing enzymesAntioksidan enzim aktiviteleri

sitokrom P450

gökkuşağı alabalığı (Oncorhynchus mykiss)

ksenobiyotik metabolize eden enzimler

malahit yeşili

Sayı

Makale detayları

Yazarlar: Azra Bozcaarmutlu Büken | Volkan Yıldırım | Canan Sapmaz | Ahmet Günbür | Hakan Türker

Yayıncı: Ataturk University

Ana Başlıklar: Sucul Toksikoloji

Dergi: The Trout Journal of Atatürk University Vol.3 Issue 1, pp.10-18

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001353086

pISSN: 2980-2539

Sayı

Yazarlar:

Azra Bozcaarmutlu Büken | Volkan Yıldırım | Canan Sapmaz | Ahmet Günbür | Hakan Türker

Yayıncı:

Ataturk University

Ana Başlıklar:

Sucul Toksikoloji

Dergi:

The Trout Journal of Atatürk University Vol.3 Issue 1, pp.10-18

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353086

pISSN:

2980-2539