Establishment of an electrochemical RNA aptamer-based biosensor to trace nanomolar concentrations of codeine

Mehdi SABERIAN; Davoud ASGARI; Yadollah OMIDI; Jaleh BARAR

Makale detayları

Establishment of an electrochemical RNA aptamer-based biosensor to trace nanomolar concentrations of codeine

Yazarlar:

Mehdi SABERIAN | Davoud ASGARI | Yadollah OMIDI | Jaleh BARAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2013 Vol.37 Issue 3, pp.366-373

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000727410

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

Codeine is an opium alkaloid with a great potential of abuse  among opioid consumers.  Detection of codeine is a routine procedure  in the military and government of some countries for personnel  recruitment.  Therefore, a specific, selective, and easy to use method  would be an important improvement in such detection procedures.  According to previous reports, short single-stranded DNA or RNA  sequences with high affinity and specificity to their targets,  aptamers, could be used for designing an accurate and specific  biosensor for codeine.  This study introduces an aptamer-based  biosensor for codeine detection in nanomolar concentrations by an  electrochemical method, and ferrocene carboxylic acid was used as the  redox molecule.  The data show an improvement in codeine detection in  comparison with the previous reports by other methods.  The fabricated  aptasensor offers a simpler and faster method with a lower limit of  detection for codeine.  The results demonstrate the reliability and  robustness of the constructed aptasensor in codeine detection and  measurement.

 

Codeine is an opium alkaloid with a great potential of abuse  among opioid consumers.  Detection of codeine is a routine procedure  in the military and government of some countries for personnel  recruitment.  Therefore, a specific, selective, and easy to use method  would be an important improvement in such detection procedures.  According to previous reports, short single-stranded DNA or RNA  sequences with high affinity and specificity to their targets,  aptamers, could be used for designing an accurate and specific  biosensor for codeine.  This study introduces an aptamer-based  biosensor for codeine detection in nanomolar concentrations by an  electrochemical method, and ferrocene carboxylic acid was used as the  redox molecule.  The data show an improvement in codeine detection in  comparison with the previous reports by other methods.  The fabricated  aptasensor offers a simpler and faster method with a lower limit of  detection for codeine.  The results demonstrate the reliability and  robustness of the constructed aptasensor in codeine detection and  measurement.

Makale detayları

Establishment of an electrochemical RNA aptamer-based biosensor to trace nanomolar concentrations of codeine

Yazarlar: Mehdi SABERIAN | Davoud ASGARI | Yadollah OMIDI | Jaleh BARAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2013 Vol.37 Issue 3, pp.366-373

Belge Tipi: Makale

Yayın Tarihi: 2/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000727410

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Mehdi SABERIAN | Davoud ASGARI | Yadollah OMIDI | Jaleh BARAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2013 Vol.37 Issue 3, pp.366-373

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000727410

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527