Enhancement of mycolytic activity of an antagonistic Bacillus subtilis through ethyl methane sulfonate (EMS) mutagenesis

Ashwini NARASIMHAN; Samantha SURESH; Deepak BIST; Srividya SHIVAKUMAR

Makale detayları

Enhancement of mycolytic activity of an antagonistic Bacillus subtilis through ethyl methane sulfonate (EMS) mutagenesis

Yazarlar:

Ashwini NARASIMHAN | Samantha SURESH | Deepak BIST | Srividya SHIVAKUMAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.323-328

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812444

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Bacillus subtilis, isolated from the chili rhizosphere, capable of producing 3 mycolytic enzymes (chitinase, b-1,3-glucanase, and cellulase), was mutated by the chemical mutagen ethyl methane sulfonate (EMS). Mutants were screened based on their antifungal ability on dual plate assay against Colletotrichum gloeosporioides OGC1, the causal agent of anthracnose disease in fruit crops. EMS mutagenesis yielded 60 isolated mutants on NA plates, of which 3 (M3, M4, and M24) showed loss of antagonism against C. gloeosporioides OGC1 and 6 (M8, M21, M22, M57, M58, and M59) exhibited increased antagonism. The remaining mutants did not show any difference in their antagonistic property. In liquid culture, the mutants exhibited varied levels of the 3 enzymes. These mutants were studied for their mycolytic enzyme activities under shake flask conditions. Of all these mutants, M57 showed a 36-fold and 4.71-fold increase in b -1,3-glucanase and cellulase activities, respectively, with a concomitant 1.95-fold increase in hydrolytic activity, followed by M59, with a 5.68-fold and 1.57-fold increase in b -1,3-glucanase and cellulase activities, respectively, and a 2.23-fold increase in hydrolytic activity as compared to the wild type B. subtilis strain. M24 exhibited a complete loss of b -1,3-glucanase and a decrease in chitinase, with a concomitant decrease in levels of hydrolytic activity with C. gloeosporioides mycelia as compared to the wild type strain. The study clearly indicated the mycolytic enzyme mediated antagonism of this strain and yielded 2 hyperproducing mutants for b -1,3-glucanase and cellulase, 2 important enzymes for various agricultural and industrial uses.

 

Bacillus subtilis, isolated from the chili rhizosphere, capable of producing 3 mycolytic enzymes (chitinase, b-1,3-glucanase, and cellulase), was mutated by the chemical mutagen ethyl methane sulfonate (EMS). Mutants were screened based on their antifungal ability on dual plate assay against Colletotrichum gloeosporioides OGC1, the causal agent of anthracnose disease in fruit crops. EMS mutagenesis yielded 60 isolated mutants on NA plates, of which 3 (M3, M4, and M24) showed loss of antagonism against C. gloeosporioides OGC1 and 6 (M8, M21, M22, M57, M58, and M59) exhibited increased antagonism. The remaining mutants did not show any difference in their antagonistic property. In liquid culture, the mutants exhibited varied levels of the 3 enzymes. These mutants were studied for their mycolytic enzyme activities under shake flask conditions. Of all these mutants, M57 showed a 36-fold and 4.71-fold increase in b -1,3-glucanase and cellulase activities, respectively, with a concomitant 1.95-fold increase in hydrolytic activity, followed by M59, with a 5.68-fold and 1.57-fold increase in b -1,3-glucanase and cellulase activities, respectively, and a 2.23-fold increase in hydrolytic activity as compared to the wild type B. subtilis strain. M24 exhibited a complete loss of b -1,3-glucanase and a decrease in chitinase, with a concomitant decrease in levels of hydrolytic activity with C. gloeosporioides mycelia as compared to the wild type strain. The study clearly indicated the mycolytic enzyme mediated antagonism of this strain and yielded 2 hyperproducing mutants for b -1,3-glucanase and cellulase, 2 important enzymes for various agricultural and industrial uses.

Makale detayları

Enhancement of mycolytic activity of an antagonistic Bacillus subtilis through ethyl methane sulfonate (EMS) mutagenesis

Yazarlar:

Ashwini NARASIMHAN | Samantha SURESH | Deepak BIST | Srividya SHIVAKUMAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.323-328

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812444

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Bacillus subtilis

mycolytic enzymes

ethyl methane sulfonate (EMS) mutagenesis

Colletotrichum gloeosporioides

Sayı

Makale detayları

Enhancement of mycolytic activity of an antagonistic Bacillus subtilis through ethyl methane sulfonate (EMS) mutagenesis

Yazarlar: Ashwini NARASIMHAN | Samantha SURESH | Deepak BIST | Srividya SHIVAKUMAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.323-328

Belge Tipi: Makale

Yayın Tarihi: 2/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000812444

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Bacillus subtilis

mycolytic enzymes

ethyl methane sulfonate (EMS) mutagenesis

Colletotrichum gloeosporioides

Sayı

Yazarlar:

Ashwini NARASIMHAN | Samantha SURESH | Deepak BIST | Srividya SHIVAKUMAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.323-328

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812444

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152