Investigation of Vitamin D Receptor Gene rs731236 Polymorphism in Turkish Rugby Players

Başak Funda Eken; Zeyneb Nur Şahin; Kate Llewellyn-waters; Gökhan Tuna; Ajlan Saç; Şehkar Oktay; Korkut Ulucan

Makale detayları

Investigation of Vitamin D Receptor Gene rs731236 Polymorphism in Turkish Rugby Players

Yazarlar:

Başak Funda Eken | Zeyneb Nur Şahin | Kate Llewellyn-waters | Gökhan Tuna | Ajlan Saç | Şehkar Oktay | Korkut Ulucan

Yayıncı:

Marmara University

Ana Başlıklar:

Spor Hekimliği

Dergi:

Eurasian Research in Sport Science Vol.8 Issue 1, pp.33-40

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291703

pISSN:

2636-8269

Anahtar kelimeler:

Özet:

 Vitamin D is an important molecule responsible for the development, contraction and regeneration of muscles, and activates its biological functions by means of its receptors, the vitamin D receptor VDR, which is a member of the nuclear receptor group. In the present study, we aimed to analyze the rs731236 distribution in Turkish rugby players. A total of 22 male players and 30 sedentary individuals as a control group participated in the study. Following DNA isolation, rs731236 polymorphisms of VDR was determined by real-time polymerase chain reaction RT- PCR. Statistical analysis was carried out by chi-square test. CC, CT and TT genotype and percentage distributions for rs731236 polymorphism in players were as 5 23, 10 45 and 7 32, respectively. Allelic distributions were found to be 20 45 for C allele, and 24 55 for T allele. For the control group; CC, CT and TT genotypes and percentages were as 4 13, 17 57 and 9 30, respectively. C allele was counted as 25 42 and 35 58 for controls. When we compare athletes and control groups in the terms of genotypes, we detected no statistically significant difference p 0.12. For the allele comparison, we detected no significant difference between groups p 0.76. Determination of gene combinations in athletes is of great importance for carrying out of the optimal training programs. Therefore, we aimed to determine the distribution of the alleles related with athletic performance on successful athletes. In our cohort, the CT genotype and T allele were more intensely determined in players.

 Vitamin D is an important molecule responsible for the development, contraction and regeneration of muscles, and activates its biological functions by means of its receptors, the vitamin D receptor VDR, which is a member of the nuclear receptor group. In the present study, we aimed to analyze the rs731236 distribution in Turkish rugby players. A total of 22 male players and 30 sedentary individuals as a control group participated in the study. Following DNA isolation, rs731236 polymorphisms of VDR was determined by real-time polymerase chain reaction RT- PCR. Statistical analysis was carried out by chi-square test. CC, CT and TT genotype and percentage distributions for rs731236 polymorphism in players were as 5 23, 10 45 and 7 32, respectively. Allelic distributions were found to be 20 45 for C allele, and 24 55 for T allele. For the control group; CC, CT and TT genotypes and percentages were as 4 13, 17 57 and 9 30, respectively. C allele was counted as 25 42 and 35 58 for controls. When we compare athletes and control groups in the terms of genotypes, we detected no statistically significant difference p 0.12. For the allele comparison, we detected no significant difference between groups p 0.76. Determination of gene combinations in athletes is of great importance for carrying out of the optimal training programs. Therefore, we aimed to determine the distribution of the alleles related with athletic performance on successful athletes. In our cohort, the CT genotype and T allele were more intensely determined in players.

 Vitamin D is an important molecule responsible for the development, contraction and regeneration of muscles, and activates its biological functions by means of its receptors, the vitamin D receptor VDR, which is a member of the nuclear receptor group. In the present study, we aimed to analyze the rs731236 distribution in Turkish rugby players. A total of 22 male players and 30 sedentary individuals as a control group participated in the study. Following DNA isolation, rs731236 polymorphisms of VDR was determined by real-time polymerase chain reaction RT- PCR. Statistical analysis was carried out by chi-square test. CC, CT and TT genotype and percentage distributions for rs731236 polymorphism in players were as 5 23, 10 45 and 7 32, respectively. Allelic distributions were found to be 20 45 for C allele, and 24 55 for T allele. For the control group; CC, CT and TT genotypes and percentages were as 4 13, 17 57 and 9 30, respectively. C allele was counted as 25 42 and 35 58 for controls. When we compare athletes and control groups in the terms of genotypes, we detected no statistically significant difference p 0.12. For the allele comparison, we detected no significant difference between groups p 0.76. Determination of gene combinations in athletes is of great importance for carrying out of the optimal training programs. Therefore, we aimed to determine the distribution of the alleles related with athletic performance on successful athletes. In our cohort, the CT genotype and T allele were more intensely determined in players.

 D vitamini kasların gelişmesinden, kasılmasından ve yenilenmesinden sorumlu önemli bir moleküldür ve nükleer reseptör grubunun bir üyesi olan vitamin D reseptörü VDR reseptörleri aracılığıyla biyolojik fonksiyonlarını aktive eder. Bu çalışmada, Türk rugby oyuncularında rs731236 dağılımını analiz etmeyi amaçladık. Çalışmaya toplam 22 erkek oyuncu ve 30 sedanter birey kontrol grubu olarak katıldı. DNA izolasyonunu takiben, VDRnin rs731236 polimorfizmleri gerçek zamanlı polimeraz zincir reaksiyonu Rt-PCR ile belirlendi. İstatistiksel analiz ki-kare testi ile yapıldı. Sporcularda rs731236 polimorfizmi için CC, CT ve TT genotip ve yüzde dağılımları sırasıyla 5 23, 10 45 ve 7 32 olarak bulundu. Alel dağılımları C aleli için 20 45 ve T aleli için 24 55 olarak bulundu. Kontrol grubu için; CC, CT ve TT genotipleri ve yüzdeleri sırasıyla 4 13, 17 57 ve 9 30dur. C aleli kontrol grubu için 25 42 ve 35 58 olarak belirlendi. Sporcular ve kontrol grubunu genotip açısından karşılaştırdığımızda istatistiksel olarak anlamlı bir fark tespit edilmedi p 0,12. Alel karşılaştırması için gruplar arasında anlamlı fark tespit edilmemiştir p 0,76. Sporcularda gen kombinasyonlarının belirlenmesi optimal antrenman programlarının yürütülmesi için büyük önem taşımaktadır. Bu nedenle atletik performansla ilgili alellerin başarılı sporcular üzerindeki dağılımını belirlemeyi amaçladık. Bizim kohortumuzda CT genotipi ve T aleli oyuncularda daha yoğun olarak belirlendi.

Makale detayları

Investigation of Vitamin D Receptor Gene rs731236 Polymorphism in Turkish Rugby Players

Yazarlar:

Başak Funda Eken | Zeyneb Nur Şahin | Kate Llewellyn-waters | Gökhan Tuna | Ajlan Saç | Şehkar Oktay | Korkut Ulucan

Yayıncı:

Marmara University

Ana Başlıklar:

Spor Hekimliği

Dergi:

Eurasian Research in Sport Science Vol.8 Issue 1, pp.33-40

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291703

pISSN:

2636-8269

VDR

polymorphism

athletes

rugby

athletic performanceVDR

polimorfizm

sporcu

ragbi

atletik performans

Sayı

Makale detayları

Yazarlar: Başak Funda Eken | Zeyneb Nur Şahin | Kate Llewellyn-waters | Gökhan Tuna | Ajlan Saç | Şehkar Oktay | Korkut Ulucan

Yayıncı: Marmara University

Ana Başlıklar: Spor Hekimliği

Dergi: Eurasian Research in Sport Science Vol.8 Issue 1, pp.33-40

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001291703

pISSN: 2636-8269

Sayı

Yazarlar:

Başak Funda Eken | Zeyneb Nur Şahin | Kate Llewellyn-waters | Gökhan Tuna | Ajlan Saç | Şehkar Oktay | Korkut Ulucan

Yayıncı:

Marmara University

Ana Başlıklar:

Spor Hekimliği

Dergi:

Eurasian Research in Sport Science Vol.8 Issue 1, pp.33-40

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291703

pISSN:

2636-8269