Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq

Maab Ibrahim Al-farwachi; Balsam Rasheed

Makale detayları

Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq

Yazarlar:

Maab Ibrahim Al-farwachi | Balsam Rasheed

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Tanı ve Teşhis

Dergi:

Veterinary Sciences and Practices Vol.19 Issue 1, pp.46-51

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316087

pISSN:

2822-3608

Anahtar kelimeler:

Özet:

 Listeriosis is a important abortive diseases in sheep , and is considered one of the most risky bacterial zoonotic disease worldwide.The study was carried out in 50 sheep flocks placed in the Nineveh governorate, Iraq during November and December 2022, a total of 300 specimens of blood , abomasum content and brain  100 eachwere collected from local breed ovine aborted fetuesin the last stage of gestation were obtained to be tested for molecular detection of Listeria monocytogenes . L monocytogenes DNA was detected in a total of 6120.3 specimens , distributed as: 3557.4 ,1524.6 and 1118.0 strains from fetal brain, abomasum and blood specimens respectively using direct genus-specific conventional polymerase chain reaction prfA gene C- PCR. Two L. monocytogenes strains  HMB1 listeriolysin, HMB 2 listeriolysin  deposited in GenBank under accession numbers LC769365.1,and LC769366.1. All L. monocytogenes strains were positive for three genes InlJ, InlA, and hlyA except actA gene was detected in 46 75.4 strains. In conclusion , L. monocytogenes is one of the important causal agents of abortion in sheep flocks in Nineveh province , Iraq, and most of the brain specimens were positive for listeric infection compared to the other specimens.

 Listeriosis is a important abortive diseases in sheep , and is considered one of the most risky bacterial zoonotic disease worldwide.The study was carried out in 50 sheep flocks placed in the Nineveh governorate, Iraq during November and December 2022, a total of 300 specimens of blood , abomasum content and brain  100 eachwere collected from local breed ovine aborted fetuesin the last stage of gestation were obtained to be tested for molecular detection of Listeria monocytogenes . L monocytogenes DNA was detected in a total of 6120.3 specimens , distributed as: 3557.4 ,1524.6 and 1118.0 strains from fetal brain, abomasum and blood specimens respectively using direct genus-specific conventional polymerase chain reaction prfA gene C- PCR. Two L. monocytogenes strains  HMB1 listeriolysin, HMB 2 listeriolysin  deposited in GenBank under accession numbers LC769365.1,and LC769366.1. All L. monocytogenes strains were positive for three genes InlJ, InlA, and hlyA except actA gene was detected in 46 75.4 strains. In conclusion , L. monocytogenes is one of the important causal agents of abortion in sheep flocks in Nineveh province , Iraq, and most of the brain specimens were positive for listeric infection compared to the other specimens.

 Listeriosis is a important abortive diseases in sheep , and is considered one of the most risky bacterial zoonotic disease worldwide.The study was carried out in 50 sheep flocks placed in the Nineveh governorate, Iraq during November and December 2022, a total of 300 specimens of blood , abomasum content and brain  100 eachwere collected from local breed ovine aborted fetuesin the last stage of gestation were obtained to be tested for molecular detection of Listeria monocytogenes . L monocytogenes DNA was detected in a total of 6120.3 specimens , distributed as: 3557.4 ,1524.6 and 1118.0 strains from fetal brain, abomasum and blood specimens respectively using direct genus-specific conventional polymerase chain reaction prfA gene C- PCR. Two L. monocytogenes strains  HMB1 listeriolysin, HMB 2 listeriolysin  deposited in GenBank under accession numbers LC769365.1,and LC769366.1. All L. monocytogenes strains were positive for three genes InlJ, InlA, and hlyA except actA gene was detected in 46 75.4 strains. In conclusion , L. monocytogenes is one of the important causal agents of abortion in sheep flocks in Nineveh province , Iraq, and most of the brain specimens were positive for listeric infection compared to the other specimens.

 Listeriosis is a important abortive diseases in sheep , and is considered one of the most risky bacterial zoonotic disease worldwide.The study was carried out in 50 sheep flocks placed in the Nineveh governorate, Iraq during November and December 2022, a total of 300 specimens of blood , abomasum content and brain  100 eachwere collected from local breed ovine aborted fetuesin the last stage of gestation were obtained to be tested for molecular detection of Listeria monocytogenes . L monocytogenes DNA was detected in a total of 6120.3 specimens , distributed as: 3557.4 ,1524.6 and 1118.0 strains from fetal brain, abomasum and blood specimens respectively using direct genus-specific conventional polymerase chain reaction prfA gene C- PCR. Two L. monocytogenes strains  HMB1 listeriolysin, HMB 2 listeriolysin  deposited in GenBank under accession numbers LC769365.1,and LC769366.1. All L. monocytogenes strains were positive for three genes InlJ, InlA, and hlyA except actA gene was detected in 46 75.4 strains. In conclusion , L. monocytogenes is one of the important causal agents of abortion in sheep flocks in Nineveh province , Iraq, and most of the brain specimens were positive for listeric infection compared to the other specimens.

Makale detayları

Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq

Yazarlar:

Maab Ibrahim Al-farwachi | Balsam Rasheed

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Tanı ve Teşhis

Dergi:

Veterinary Sciences and Practices Vol.19 Issue 1, pp.46-51

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316087

pISSN:

2822-3608

Listerosis

Abortion

Ovine

Listeriolysin

IraqKeywords: Listerosis

Abortion

ovine

listeriolysin

Iraq.

Sayı

Makale detayları

Yazarlar: Maab Ibrahim Al-farwachi | Balsam Rasheed

Yayıncı: Ataturk University

Ana Başlıklar: Veteriner Tanı ve Teşhis

Dergi: Veterinary Sciences and Practices Vol.19 Issue 1, pp.46-51

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001316087

pISSN: 2822-3608

Sayı

Yazarlar:

Maab Ibrahim Al-farwachi | Balsam Rasheed

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Tanı ve Teşhis

Dergi:

Veterinary Sciences and Practices Vol.19 Issue 1, pp.46-51

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316087

pISSN:

2822-3608