Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations

Ayşe Esra Karadağ; Betül Demirci; Sevde Nur Biltekin

Makale detayları

Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations

Yazarlar:

Ayşe Esra Karadağ | Betül Demirci | Sevde Nur Biltekin

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 5, pp.1820-1828

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347602

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Salvia sclarea L. and Citrus aurantium L. are used ethnobotanically for wound treatment. The aim of this study was to evaluate phytochemical composition of Salvia sclarea L. and Citrus aurantium L. leaf essential oils and wound healing activities of their combinations. The phytochemical analysis was performed gas chromatography GC and mass spectroscopy MS. Major components of S. sclarea leaf essential oil was confirmed both by GC-FID and GCMS as 67 linalyl acetate, 20.2 linalool, 2.6α-terpineole, respectively. The major components of C. aurantium leaf essential oil was also confirmed as 55 linalyl acetate, 23.4 linalool, 5.4α-terpineole, respectively. Combinations of essential oils were loaded into Carbopol gel formulations. Wound healing activity of essential oil and combinations was investigated using in vitro scratch assay. As a result of the study, it was determined that the most effective wound healing concentration for Salvia sclarea essential oil was 50 µgmL. The most effective concentration of Citrus aurantifolia essential oil was determined as 12.5 µgmL. The aim of the study is to investigate the wound healing activities of the tested essential oils individually and in combination. The fact that no activity is observed in empty formulations is evidence that the activity of the loaded formulations does not come from empty formulations. It was observed that the loaded formulations showed better effects than the control Madecassol. Studies need to be confirmed by working under in vivo conditions.

 Salvia sclarea L. and Citrus aurantium L. are used ethnobotanically for wound treatment. The aim of this study was to evaluate phytochemical composition of Salvia sclarea L. and Citrus aurantium L. leaf essential oils and wound healing activities of their combinations. The phytochemical analysis was performed gas chromatography GC and mass spectroscopy MS. Major components of S. sclarea leaf essential oil was confirmed both by GC-FID and GCMS as 67 linalyl acetate, 20.2 linalool, 2.6α-terpineole, respectively. The major components of C. aurantium leaf essential oil was also confirmed as 55 linalyl acetate, 23.4 linalool, 5.4α-terpineole, respectively. Combinations of essential oils were loaded into Carbopol gel formulations. Wound healing activity of essential oil and combinations was investigated using in vitro scratch assay. As a result of the study, it was determined that the most effective wound healing concentration for Salvia sclarea essential oil was 50 µgmL. The most effective concentration of Citrus aurantifolia essential oil was determined as 12.5 µgmL. The aim of the study is to investigate the wound healing activities of the tested essential oils individually and in combination. The fact that no activity is observed in empty formulations is evidence that the activity of the loaded formulations does not come from empty formulations. It was observed that the loaded formulations showed better effects than the control Madecassol. Studies need to be confirmed by working under in vivo conditions.

 Salvia sclarea L. and Citrus aurantium L. are used ethnobotanically for wound treatment. The aim of this study was to evaluate phytochemical composition of Salvia sclarea L. and Citrus aurantium L. leaf essential oils and wound healing activities of their combinations. The phytochemical analysis was performed gas chromatography GC and mass spectroscopy MS. Major components of S. sclarea leaf essential oil was confirmed both by GC-FID and GCMS as 67 linalyl acetate, 20.2 linalool, 2.6α-terpineole, respectively. The major components of C. aurantium leaf essential oil was also confirmed as 55 linalyl acetate, 23.4 linalool, 5.4α-terpineole, respectively. Combinations of essential oils were loaded into Carbopol gel formulations. Wound healing activity of essential oil and combinations was investigated using in vitro scratch assay. As a result of the study, it was determined that the most effective wound healing concentration for Salvia sclarea essential oil was 50 µgmL. The most effective concentration of Citrus aurantifolia essential oil was determined as 12.5 µgmL. The aim of the study is to investigate the wound healing activities of the tested essential oils individually and in combination. The fact that no activity is observed in empty formulations is evidence that the activity of the loaded formulations does not come from empty formulations. It was observed that the loaded formulations showed better effects than the control Madecassol. Studies need to be confirmed by working under in vivo conditions.

 Salvia sclarea L. and Citrus aurantium L. are used ethnobotanically for wound treatment. The aim of this study was to evaluate phytochemical composition of Salvia sclarea L. and Citrus aurantium L. leaf essential oils and wound healing activities of their combinations. The phytochemical analysis was performed gas chromatography GC and mass spectroscopy MS. Major components of S. sclarea leaf essential oil was confirmed both by GC-FID and GCMS as 67 linalyl acetate, 20.2 linalool, 2.6α-terpineole, respectively. The major components of C. aurantium leaf essential oil was also confirmed as 55 linalyl acetate, 23.4 linalool, 5.4α-terpineole, respectively. Combinations of essential oils were loaded into Carbopol gel formulations. Wound healing activity of essential oil and combinations was investigated using in vitro scratch assay. As a result of the study, it was determined that the most effective wound healing concentration for Salvia sclarea essential oil was 50 µgmL. The most effective concentration of Citrus aurantifolia essential oil was determined as 12.5 µgmL. The aim of the study is to investigate the wound healing activities of the tested essential oils individually and in combination. The fact that no activity is observed in empty formulations is evidence that the activity of the loaded formulations does not come from empty formulations. It was observed that the loaded formulations showed better effects than the control Madecassol. Studies need to be confirmed by working under in vivo conditions.

Makale detayları

Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations

Yazarlar: Ayşe Esra Karadağ | Betül Demirci | Sevde Nur Biltekin

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.28 Issue 5, pp.1820-1828

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347602

pISSN: 2630-6344

Salvia sclarea

Citrus aurantium

wound healing

essential oil

Sayı

Yazarlar:

Ayşe Esra Karadağ | Betül Demirci | Sevde Nur Biltekin

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 5, pp.1820-1828

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347602

pISSN:

2630-6344