One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and  its application for the detection of foot-and-mouth-disease virus and its serotypes

Sidra MARYAM; Tahir RASHEED; Asma LATIF; Rabaab ZAHRA; Aamer Bin ZAHUR; Aitezaz AHSAN; Muhammad AFZAL; Umer FAROOQ

Makale detayları

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

Yazarlar:

Sidra MARYAM | Tahir RASHEED | Asma LATIF | Rabaab ZAHRA | Aamer Bin ZAHUR | Aitezaz AHSAN | Muhammad AFZAL | Umer FAROOQ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2017 Vol.41 Issue 3, pp.435-443

Belge Tipi:

Makale

Yayın Tarihi:

6/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000764986

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

Availability of a rapid and sensitive diagnostic technique is key in successful prevention and control of infectious diseases like foot-and-mouth disease (FMD). Existing conventional diagnostic tests for FMD are laborious and time-consuming with low sensitivity and specificity. Molecular-based techniques are costly and difficult, involving refined apparatus like a thermal cycler. In the present study, the technique of real-time loop-mediated isothermal amplification (RT-LAMP) was standardized for diagnosing FMDV and its serotypes, evaluated using field samples, and compared with the existing real-time PCR in Pakistan. RT-LAMP amplified the target 3D gene using specific primers at 65 °C for 60 min and the VP1 gene using serotype specific primers at 63 °C for 60 min. A total of 38 samples out of 50 were positive by RT-LAMP and identified serotypes were A (n = 15), O (n = 15), and Asia-1 (n = 8). The efficiency of RT-LAMP in this study was highest for serotype Asia-1 (80.9%) followed by serotype A (73.4%) and O (62.35).

Makale detayları

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

Yazarlar: Sidra MARYAM | Tahir RASHEED | Asma LATIF | Rabaab ZAHRA | Aamer Bin ZAHUR | Aitezaz AHSAN | Muhammad AFZAL | Umer FAROOQ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2017 Vol.41 Issue 3, pp.435-443

Belge Tipi: Makale

Yayın Tarihi: 6/2017

Belge Dili: İngilizce

Document ID (Org No.): 2000764986

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Sidra MARYAM | Tahir RASHEED | Asma LATIF | Rabaab ZAHRA | Aamer Bin ZAHUR | Aitezaz AHSAN | Muhammad AFZAL | Umer FAROOQ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2017 Vol.41 Issue 3, pp.435-443

Belge Tipi:

Makale

Yayın Tarihi:

6/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000764986

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128