Isolation, Identification and Antibiotic Sensitivity of Pasteurella Species In Cattle Slaughtered in Slaughterhouses in Kütahya Province

Seydi Mehmet Arslan; Beytullah Kenar

Makale detayları

Isolation, Identification and Antibiotic Sensitivity of Pasteurella Species In Cattle Slaughtered in Slaughterhouses in Kütahya Province

Yazarlar:

Seydi Mehmet Arslan | Beytullah Kenar

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Cerrahi

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 2, pp.127-130

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287480

pISSN:

1308-1594

Anahtar kelimeler:

Özet:

 In this study, it was aimed to isolate and identify Pasteurella species, which is one of the most important bacterial agents that cause bovine pneumonia, and to determine their antibiotic susceptibility as the most accurate approach in treatment. For this purpose, 210 lung samples were taken from cattle slaughtered in slaughterhouses in Kütahya. Sowing was performed on blood agar, Eosin methylene blue agar and MacConkey agar from the samples and 89 42 Gram negative bacteria were isolated. 17 bacteria showing the characteristics of Pasteurella species were identified in the automated Vitek 2 device. As a result of the identification, two 0.95 isolates were found to be Pasteurella multocida. Antibiotic susceptibility of the isolates was investigated using the Kirby-Bauer disk diffusion test. It was observed that the isolates were 100 sensitive to amoxicillin clavulanic acid, enrofloxacin, ciprofloxacin and ceftiofur, 50 sensitive to danofloxacin, 50 sensitive at increased dosage, and 100 resistant to sulfamethoxazole-trimethoprim and tetracycline.

 In this study, it was aimed to isolate and identify Pasteurella species, which is one of the most important bacterial agents that cause bovine pneumonia, and to determine their antibiotic susceptibility as the most accurate approach in treatment. For this purpose, 210 lung samples were taken from cattle slaughtered in slaughterhouses in Kütahya. Sowing was performed on blood agar, Eosin methylene blue agar and MacConkey agar from the samples and 89 42 Gram negative bacteria were isolated. 17 bacteria showing the characteristics of Pasteurella species were identified in the automated Vitek 2 device. As a result of the identification, two 0.95 isolates were found to be Pasteurella multocida. Antibiotic susceptibility of the isolates was investigated using the Kirby-Bauer disk diffusion test. It was observed that the isolates were 100 sensitive to amoxicillin clavulanic acid, enrofloxacin, ciprofloxacin and ceftiofur, 50 sensitive to danofloxacin, 50 sensitive at increased dosage, and 100 resistant to sulfamethoxazole-trimethoprim and tetracycline.

 In this study, it was aimed to isolate and identify Pasteurella species, which is one of the most important bacterial agents that cause bovine pneumonia, and to determine their antibiotic susceptibility as the most accurate approach in treatment. For this purpose, 210 lung samples were taken from cattle slaughtered in slaughterhouses in Kütahya. Sowing was performed on blood agar, Eosin methylene blue agar and MacConkey agar from the samples and 89 42 Gram negative bacteria were isolated. 17 bacteria showing the characteristics of Pasteurella species were identified in the automated Vitek 2 device. As a result of the identification, two 0.95 isolates were found to be Pasteurella multocida. Antibiotic susceptibility of the isolates was investigated using the Kirby-Bauer disk diffusion test. It was observed that the isolates were 100 sensitive to amoxicillin clavulanic acid, enrofloxacin, ciprofloxacin and ceftiofur, 50 sensitive to danofloxacin, 50 sensitive at increased dosage, and 100 resistant to sulfamethoxazole-trimethoprim and tetracycline.

 Yapılan bu çalışmada sığır pnömonilerine sebep olan en önemli bakteriyel etkenlerden biri olan Pasteurella türlerini izole ve identifiye ederek tedavide en doğru yaklaşım olarak antibiyotik duyarlılıklarını belirlemek amaçlanmıştır. Bu amaçla Kütahya ilindeki mezbahalarda kesilen sığırlardan 210 akciğer örneği alındı. Örneklerden kanlı agar, Eosin methylene blue agar ve MacConkey agara ekimler gerçekleştirildi ve 89 42 Gram negatif bakteri izole edildi. Pasteurella türlerinin özelliklerini gösteren 17 bakteri otomatize Vitek 2 cihazında identifiye edildi. İdentifikasyon sonucu iki 0,95 izolatun Pasteurella multocida olduğu tespit edildi. İzolatların antibiyotik duyarlılıkları Kirby-Bauer disk difüzyon testi kullanılarak araştırıldı. İzolatların amoksisilin klavulanik asit, enrofloksasin, siprofloksasin ve seftiofura 100 oranında duyarlı, danofloksasine ise 50 duyarlı 50 artırılmış dozajda duyarlı olduğu gözlemlenirken, sülfametaksazol-trimetoprim ve tetrasikline 100 oranında dirençli olduğu tespit edildi.

Makale detayları

Isolation, Identification and Antibiotic Sensitivity of Pasteurella Species In Cattle Slaughtered in Slaughterhouses in Kütahya Province

Yazarlar:

Seydi Mehmet Arslan | Beytullah Kenar

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Cerrahi

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 2, pp.127-130

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287480

pISSN:

1308-1594

Antibiotic sensitivity test

cattle

slaughterhouse

Pasteurella species

Antibiyotik duyarlılık testi

sığır

kesimhane

Pasteurella türleri

Sayı

Makale detayları

Yazarlar: Seydi Mehmet Arslan | Beytullah Kenar

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Veteriner Cerrahi

Dergi: Kocatepe Veteriner Dergisi Vol.16 Issue 2, pp.127-130

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001287480

pISSN: 1308-1594

Sayı

Yazarlar:

Seydi Mehmet Arslan | Beytullah Kenar

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Cerrahi

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 2, pp.127-130

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287480

pISSN:

1308-1594