Effects of Sequential Sub-Culturing on the Survival and Enzyme Activity of Neocallimastix hurleyensis

Mehmet Sait EKİNCİ; Emin ÖZKÖSE; İsmail AKYOL

Makale detayları

Effects of Sequential Sub-Culturing on the Survival and Enzyme Activity of Neocallimastix hurleyensis

Yazarlar:

Mehmet Sait EKİNCİ | Emin ÖZKÖSE | İsmail AKYOL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2006 Vol.30 Issue 3, pp.157-162

Belge Tipi:

Makale

Yayın Tarihi:

2/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000813370

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

This study investigated the long-term adaptation of a rumen anaerobic fungus, Neocallimastix hurleyensis, grown on each of 3 different substrates as the sole energy source. The rumen anaerobic fungus was cultivated on Avicel, Xylan and glucose for up to 31 subcultures, and its survival and enzymatic activity were monitored. The fungus was able to survive on each of these substrates. Significant increases in the xylanase, avicelase and carboxymethylcellulase (CMCase) activities of N. hurleyensis were observed after 31 subcultures on Avicel and Xylan. However, there was a significant decrease in these enzyme activities after 31 consecutive subcultures of the N. hurleyensis fungus on the medium containing glucose as the sole energy source.

 

This study investigated the long-term adaptation of a rumen anaerobic fungus, Neocallimastix hurleyensis, grown on each of 3 different substrates as the sole energy source. The rumen anaerobic fungus was cultivated on Avicel, Xylan and glucose for up to 31 subcultures, and its survival and enzymatic activity were monitored. The fungus was able to survive on each of these substrates. Significant increases in the xylanase, avicelase and carboxymethylcellulase (CMCase) activities of N. hurleyensis were observed after 31 subcultures on Avicel and Xylan. However, there was a significant decrease in these enzyme activities after 31 consecutive subcultures of the N. hurleyensis fungus on the medium containing glucose as the sole energy source.

Makale detayları

Effects of Sequential Sub-Culturing on the Survival and Enzyme Activity of Neocallimastix hurleyensis

Yazarlar:

Mehmet Sait EKİNCİ | Emin ÖZKÖSE | İsmail AKYOL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2006 Vol.30 Issue 3, pp.157-162

Belge Tipi:

Makale

Yayın Tarihi:

2/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000813370

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anaerobic fungi

Neocallimastix hurleyensis

xylanase

avicelase

CMCase

Sayı

Makale detayları

Effects of Sequential Sub-Culturing on the Survival and Enzyme Activity of Neocallimastix hurleyensis

Yazarlar: Mehmet Sait EKİNCİ | Emin ÖZKÖSE | İsmail AKYOL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2006 Vol.30 Issue 3, pp.157-162

Belge Tipi: Makale

Yayın Tarihi: 2/2006

Belge Dili: Türkçe

Document ID (Org No.): 2000813370

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Anaerobic fungi

Neocallimastix hurleyensis

xylanase

avicelase

CMCase

Sayı

Yazarlar:

Mehmet Sait EKİNCİ | Emin ÖZKÖSE | İsmail AKYOL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2006 Vol.30 Issue 3, pp.157-162

Belge Tipi:

Makale

Yayın Tarihi:

2/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000813370

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152