The investigation of the effect of two different heavy metals (copper and zinc) on the medicinal leeches (Hirudo verbana)

Erdal Yılmaz; Ebubekir Atici

Makale detayları

The investigation of the effect of two different heavy metals (copper and zinc) on the medicinal leeches (Hirudo verbana)

Yazarlar:

Erdal Yılmaz | Ebubekir Atici

Yayıncı:

Yozgat Bozok University

Ana Başlıklar:

Veteriner Mikrobiyolojisi

Dergi:

Bozok Veterinary Sciences Vol.5 Issue 2, pp.70-72

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001334819

pISSN:

2757-5179

Anahtar kelimeler:

Özet:

 In the study, the effects of different concentrations of zinc and copper metals on medicinal leeches were investigated. Fifteen medicinal leeches Hirudo verbana, which were stocked in containers with 10 liters of water, were exposed to two different doses of copper and zinc metals, which were determined below the lethal doses. While applied doses of zinc and copper were 3.0 mgL, 12 mgL, and 0.15 mgL and 0,6 mgL during 96 hours, respectively. Applied doses of zinc and copper were 0,5 mgL and 2,0 mgL, and 0,02 mgL and 0,08 mgL for 30 days applications, respectively. After these applications, antimicrobial characteristics of the leech saliva, heavy metal deposition and malondialdehyde MDA levels were analysed. It was determined that leech saliva extract did not show any antimicrobial activity on fish pathogens, Lactococcus garviae and Yersinia ruckeri. Also, It has been found that when medicinal leeches exposed to zinc and copper metals, heavy metals concentrations in tissues of the medicinal leeches increased. In addition, it was found that malondialdehyde values in leech saliva increased when zinc and copper were exposed at different concentrations and durations. It was revealed from the data that medicinal leech of the Hirudo verbana species is a good biomonitor organism for zinc and copper heavy metals.

 In the study, the effects of different concentrations of zinc and copper metals on medicinal leeches were investigated. Fifteen medicinal leeches Hirudo verbana, which were stocked in containers with 10 liters of water, were exposed to two different doses of copper and zinc metals, which were determined below the lethal doses. While applied doses of zinc and copper were 3.0 mgL, 12 mgL, and 0.15 mgL and 0,6 mgL during 96 hours, respectively. Applied doses of zinc and copper were 0,5 mgL and 2,0 mgL, and 0,02 mgL and 0,08 mgL for 30 days applications, respectively. After these applications, antimicrobial characteristics of the leech saliva, heavy metal deposition and malondialdehyde MDA levels were analysed. It was determined that leech saliva extract did not show any antimicrobial activity on fish pathogens, Lactococcus garviae and Yersinia ruckeri. Also, It has been found that when medicinal leeches exposed to zinc and copper metals, heavy metals concentrations in tissues of the medicinal leeches increased. In addition, it was found that malondialdehyde values in leech saliva increased when zinc and copper were exposed at different concentrations and durations. It was revealed from the data that medicinal leech of the Hirudo verbana species is a good biomonitor organism for zinc and copper heavy metals.

 In the study, the effects of different concentrations of zinc and copper metals on medicinal leeches were investigated. Fifteen medicinal leeches Hirudo verbana, which were stocked in containers with 10 liters of water, were exposed to two different doses of copper and zinc metals, which were determined below the lethal doses. While applied doses of zinc and copper were 3.0 mgL, 12 mgL, and 0.15 mgL and 0,6 mgL during 96 hours, respectively. Applied doses of zinc and copper were 0,5 mgL and 2,0 mgL, and 0,02 mgL and 0,08 mgL for 30 days applications, respectively. After these applications, antimicrobial characteristics of the leech saliva, heavy metal deposition and malondialdehyde MDA levels were analysed. It was determined that leech saliva extract did not show any antimicrobial activity on fish pathogens, Lactococcus garviae and Yersinia ruckeri. Also, It has been found that when medicinal leeches exposed to zinc and copper metals, heavy metals concentrations in tissues of the medicinal leeches increased. In addition, it was found that malondialdehyde values in leech saliva increased when zinc and copper were exposed at different concentrations and durations. It was revealed from the data that medicinal leech of the Hirudo verbana species is a good biomonitor organism for zinc and copper heavy metals.

 Araştırmada çinko ve bakır metallerinin farklı konsantrasyonlarının tıbbi sülükler üzerindeki etkileri incelenmiştir. 10 litrelik kaplara 15 adet olacak şekilde stoklanan tıbbi sülükler Hirudo verbana bakır ve çinko metallerinin letal dozların altında belirlenen farklı iki doza maruz bırakılmıştır. Çinko ve bakır için 96 saatlik muamele dozları sırası ile 3.0 mgL ile 12 mgL ve 0.15 mgL ile 0.6 mgL iken; 30 günlük muamelelerde 0.5 mgL ile 2.0 mgL ve 0.02 mgL ile 0.08 mgL olarak uygulanmıştır. Bu uygulamalardan sonra sülüklerden alınan salya örneklerinin antimikrobiyal karakteristiği, ağır metal birikimleri ve malondialdehyde MDA düzeyleri analiz edildi. Çalışmada balık patojenlerinden Lactococcus garviae ve Yersinia ruckeri ye karşı sülük salyasının herhangi bir antimikrobiyal etki göstermediği belirlenmiştir. Tıbbi sülüklerin çinko ve bakır metallerine maruz kaldıklarında dokularında depolanan ağır metal miktarlarının arttığı tespit edilmiştir. Ayrıca çinko ve bakırın farklı konsantrasyon ve sürelerde maruziyetinde sülük salyasında malondialdehit değerlerinin de artış gösterdiği bulunmuştur. Elde edilen verilerden Hirudo verbana türü tıbbi sülüğün çinko ve bakır ağır metalleri için iyi bir biyo-monitör organizma olduğu ortaya konulmuştur.

Makale detayları

The investigation of the effect of two different heavy metals (copper and zinc) on the medicinal leeches (Hirudo verbana)

Yazarlar:

Erdal Yılmaz | Ebubekir Atici

Yayıncı:

Yozgat Bozok University

Ana Başlıklar:

Veteriner Mikrobiyolojisi

Dergi:

Bozok Veterinary Sciences Vol.5 Issue 2, pp.70-72

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001334819

pISSN:

2757-5179

Antimicrobial

heavy metal

Hirudo verbana

medicinal leech

malondialdehydeAğır metal

antimikrobiyal

Hirudo verbana

malondialdehit

tıbbi sülük

Sayı

Makale detayları

Yazarlar: Erdal Yılmaz | Ebubekir Atici

Yayıncı: Yozgat Bozok University

Ana Başlıklar: Veteriner Mikrobiyolojisi

Dergi: Bozok Veterinary Sciences Vol.5 Issue 2, pp.70-72

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001334819

pISSN: 2757-5179

Sayı

Yazarlar:

Erdal Yılmaz | Ebubekir Atici

Yayıncı:

Yozgat Bozok University

Ana Başlıklar:

Veteriner Mikrobiyolojisi

Dergi:

Bozok Veterinary Sciences Vol.5 Issue 2, pp.70-72

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001334819

pISSN:

2757-5179