Virological and Serological Investigation of Feline Coronavirus Infection in Cats

Ahmet Topçu; Yakup Yıldırım

Makale detayları

Virological and Serological Investigation of Feline Coronavirus Infection in Cats

Yazarlar:

Ahmet Topçu | Yakup Yıldırım

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Veterinary Virology

Dergi:

Mehmet Akif Ersoy University Journal of Health Sciences Institute Vol.12 Issue 2, pp.46-52

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326869

pISSN:

2148-2837

Anahtar kelimeler:

Özet:

 Feline coronavirus infection is an important viral disease affecting feline health. In this study, feline coronavirus infection FCoV in cats was investigated using virological and serological methods. For this purpose, both blood and stool samples were taken from 60 cats aged six months or older with or without clinical signs in different races, sexes, ages and social environments, which were not vaccinated against the aforementioned infection, which were kept as pets at home. Collected blood samples were checked for FCoV antibodies by applying FCoV rapid test-antibody Ab and indirect Enzyme Linked Immunosorbent Assay ELISA methods. Similarly, stool samples taken from the same cats were checked for the presence of FCoV antigen using the FCoV rapid test-antigen Ag method. Of the 60 cat blood serum samples analyzed, 21 35 were determined to be seropositive by rapid test-Ab method and 41 68.33 by indirect ELISA method. The difference between the antibody positivity rates determined as a result of the indirect ELISA method and the rapid test-Ab method was found to be statistically significant P0.001. The presence of FCoV in 60 stool samples collected was investigated by rapid test-Ag method and 1 sample 1.67 was found to be antigen positive. As a result of this research, the presenceprevalence of FCoV infection in owned cats was revealed both virologically and serologically. In addition, the indirect ELISA method was found to be more sensitive and reliable than the rapid test-Ab method in the serological diagnosis of FCoV infection.

 Feline coronavirus infection is an important viral disease affecting feline health. In this study, feline coronavirus infection FCoV in cats was investigated using virological and serological methods. For this purpose, both blood and stool samples were taken from 60 cats aged six months or older with or without clinical signs in different races, sexes, ages and social environments, which were not vaccinated against the aforementioned infection, which were kept as pets at home. Collected blood samples were checked for FCoV antibodies by applying FCoV rapid test-antibody Ab and indirect Enzyme Linked Immunosorbent Assay ELISA methods. Similarly, stool samples taken from the same cats were checked for the presence of FCoV antigen using the FCoV rapid test-antigen Ag method. Of the 60 cat blood serum samples analyzed, 21 35 were determined to be seropositive by rapid test-Ab method and 41 68.33 by indirect ELISA method. The difference between the antibody positivity rates determined as a result of the indirect ELISA method and the rapid test-Ab method was found to be statistically significant P0.001. The presence of FCoV in 60 stool samples collected was investigated by rapid test-Ag method and 1 sample 1.67 was found to be antigen positive. As a result of this research, the presenceprevalence of FCoV infection in owned cats was revealed both virologically and serologically. In addition, the indirect ELISA method was found to be more sensitive and reliable than the rapid test-Ab method in the serological diagnosis of FCoV infection.

 Feline coronavirus infection is an important viral disease affecting feline health. In this study, feline coronavirus infection FCoV in cats was investigated using virological and serological methods. For this purpose, both blood and stool samples were taken from 60 cats aged six months or older with or without clinical signs in different races, sexes, ages and social environments, which were not vaccinated against the aforementioned infection, which were kept as pets at home. Collected blood samples were checked for FCoV antibodies by applying FCoV rapid test-antibody Ab and indirect Enzyme Linked Immunosorbent Assay ELISA methods. Similarly, stool samples taken from the same cats were checked for the presence of FCoV antigen using the FCoV rapid test-antigen Ag method. Of the 60 cat blood serum samples analyzed, 21 35 were determined to be seropositive by rapid test-Ab method and 41 68.33 by indirect ELISA method. The difference between the antibody positivity rates determined as a result of the indirect ELISA method and the rapid test-Ab method was found to be statistically significant P0.001. The presence of FCoV in 60 stool samples collected was investigated by rapid test-Ag method and 1 sample 1.67 was found to be antigen positive. As a result of this research, the presenceprevalence of FCoV infection in owned cats was revealed both virologically and serologically. In addition, the indirect ELISA method was found to be more sensitive and reliable than the rapid test-Ab method in the serological diagnosis of FCoV infection.

 Feline coronavirus enfeksyonu kedi sağlığını etkileyen önemli viral bir hastalıktır. Bu çalışmada, kedilerde feline coronavirus enfeksiyonunun varlığı FCoV virolojik ve serolojik yöntemler kullanılarak araştırıldı. Bu amaçla söz konusu enfeksiyona karşı aşılanmamış farklı ırk, cinsiyet, yaş ve sosyal çevrede bulunan klinik bulgu gösteren veya göstermeyen altı ay yaştan büyük sahipli 60 kediden hem kan hem de dışkı örneklemesi yapıldı. Toplanan kan numunelerine FCoV antikor Ab rapid test ve indirekt Enzyme Linked Immunosorbent Assay ELISA yöntemleri uygulanarak FCoV antikorları yönünden kontrol edildi. Benzer şekilde aynı kedilerden alınan dışkı numuneleri de FCoV antijen Ag rapid test yöntemi kullanılarak FCoV antijen varlığı yönünden kontrol edildi. Analizleri yapılan 60 kedi kan serumu numunesinin rapid test-Ab yöntemi ile 21i 35, indirekt ELISA yöntemiyle ise 41i 68,33 seropozitif olarak belirlendi. İndirekt ELISA yöntemi ile rapid test-Ab yöntemi sonucunda belirlenen antikor pozitiflik oranları arasındaki farklılığın istatistiksel olarak önemli P0.001 olduğu tespit edildi. Toplanan 60 adet dışkı örneğinde de FCoV varlığı rapid test-Ag yöntemi ile araştırıldı ve bir numunede 1,67 antijen pozitifliği belirlendi. Bu araştırma sonucunda sahipli kedilerde FCoV enfeksiyonunun varlığıyaygınlığı hem virolojik hem de serolojik olarak ortaya koyulmuştur. Ayrıca FCoV enfeksiyonunun serolojik tanısında indirekt ELISA yönteminin rapid test-Ab yöntemine göre çok daha duyarlı ve güvenilir olduğu tespit edilmiştir.

Makale detayları

Virological and Serological Investigation of Feline Coronavirus Infection in Cats

Yazarlar:

Ahmet Topçu | Yakup Yıldırım

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Veterinary Virology

Dergi:

Mehmet Akif Ersoy University Journal of Health Sciences Institute Vol.12 Issue 2, pp.46-52

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326869

pISSN:

2148-2837

Antigen

Antibody

ELISA

Feline

Feline Coronavirus

Rapid TestAntijen

Antikor

ELISA

Feline Coronavirus

Kedi

Rapid Test

Sayı

Makale detayları

Yazarlar: Ahmet Topçu | Yakup Yıldırım

Yayıncı: Burdur Mehmet Akif Ersoy University

Ana Başlıklar: Veterinary Virology

Dergi: Mehmet Akif Ersoy University Journal of Health Sciences Institute Vol.12 Issue 2, pp.46-52

Belge Tipi: Makale

Yayın Tarihi: 5/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001326869

pISSN: 2148-2837

Sayı

Yazarlar:

Ahmet Topçu | Yakup Yıldırım

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Veterinary Virology

Dergi:

Mehmet Akif Ersoy University Journal of Health Sciences Institute Vol.12 Issue 2, pp.46-52

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326869

pISSN:

2148-2837