Osteogenic differentiation of canine adipose derived mesenchymal stem cells on B‐TCP and B‐TCP/Collagen biomaterials

Özge Özgenç; Asuman Özen

Makale detayları

Osteogenic differentiation of canine adipose derived mesenchymal stem cells on B‐TCP and B‐TCP/Collagen biomaterials

Yazarlar:

Özge Özgenç | Asuman Özen

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Histology and Embryology

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 2, pp.125-134

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311976

pISSN:

1300-0861

Anahtar kelimeler:

Özet:

 Mesenchymal stem cells are adult stem cells that can differentiate into osteogenic, chondrogenic, adipogenic and myogenic lineages. In orthopedics and traumatology, mesenchymal stem cells, combined with biomaterials, are used mainly for treating bone fractures and diseases in humans and animals. This study aims to promote the growth, proliferation, and osteogenic differentiation of mesenchymal stem cells isolated from the adipose tissue of canines on BTCP Betatricalcium phosphate and BTCPCollagen biomaterials. MTT analysis was performed to test the cell adhesion and proliferation on BTCP and BTCPCollagen biomaterials used to mimic the extracellular matrix of threedimensional bone tissue. Scanning electron microscope analysis was performed to show general surface characters of BTCP and BTCP Collagen biomaterials. The osteoinductive capacities of the BTCP and BTCPCollagen biomaterials were determined by alkaline phosphatase and Von Kossa stainings, and RTPCR analysis. The ALP activity of the BTCPCol containing material was significantly higher than the BTCP in the early days. In terms of gene expression, there were no significant differences except 14thday SPARC gene expression. The results of Von Kossa staining indicated that BTCP Col has above the desired level degradation capacity. As a result of this research, although it is advantageous in terms of alkaline phosphatase activity and osteogenic gene expression compared to BTCP material, it is thought that BTCPCollagen biomaterial should be developed for use in bone tissue engineering due to its high degradation property.

 Mesenchymal stem cells are adult stem cells that can differentiate into osteogenic, chondrogenic, adipogenic and myogenic lineages. In orthopedics and traumatology, mesenchymal stem cells, combined with biomaterials, are used mainly for treating bone fractures and diseases in humans and animals. This study aims to promote the growth, proliferation, and osteogenic differentiation of mesenchymal stem cells isolated from the adipose tissue of canines on BTCP Betatricalcium phosphate and BTCPCollagen biomaterials. MTT analysis was performed to test the cell adhesion and proliferation on BTCP and BTCPCollagen biomaterials used to mimic the extracellular matrix of threedimensional bone tissue. Scanning electron microscope analysis was performed to show general surface characters of BTCP and BTCP Collagen biomaterials. The osteoinductive capacities of the BTCP and BTCPCollagen biomaterials were determined by alkaline phosphatase and Von Kossa stainings, and RTPCR analysis. The ALP activity of the BTCPCol containing material was significantly higher than the BTCP in the early days. In terms of gene expression, there were no significant differences except 14thday SPARC gene expression. The results of Von Kossa staining indicated that BTCP Col has above the desired level degradation capacity. As a result of this research, although it is advantageous in terms of alkaline phosphatase activity and osteogenic gene expression compared to BTCP material, it is thought that BTCPCollagen biomaterial should be developed for use in bone tissue engineering due to its high degradation property.

 Mesenchymal stem cells are adult stem cells that can differentiate into osteogenic, chondrogenic, adipogenic and myogenic lineages. In orthopedics and traumatology, mesenchymal stem cells, combined with biomaterials, are used mainly for treating bone fractures and diseases in humans and animals. This study aims to promote the growth, proliferation, and osteogenic differentiation of mesenchymal stem cells isolated from the adipose tissue of canines on BTCP Betatricalcium phosphate and BTCPCollagen biomaterials. MTT analysis was performed to test the cell adhesion and proliferation on BTCP and BTCPCollagen biomaterials used to mimic the extracellular matrix of threedimensional bone tissue. Scanning electron microscope analysis was performed to show general surface characters of BTCP and BTCP Collagen biomaterials. The osteoinductive capacities of the BTCP and BTCPCollagen biomaterials were determined by alkaline phosphatase and Von Kossa stainings, and RTPCR analysis. The ALP activity of the BTCPCol containing material was significantly higher than the BTCP in the early days. In terms of gene expression, there were no significant differences except 14thday SPARC gene expression. The results of Von Kossa staining indicated that BTCP Col has above the desired level degradation capacity. As a result of this research, although it is advantageous in terms of alkaline phosphatase activity and osteogenic gene expression compared to BTCP material, it is thought that BTCPCollagen biomaterial should be developed for use in bone tissue engineering due to its high degradation property.

 Mesenchymal stem cells are adult stem cells that can differentiate into osteogenic, chondrogenic, adipogenic and myogenic lineages. In orthopedics and traumatology, mesenchymal stem cells, combined with biomaterials, are used mainly for treating bone fractures and diseases in humans and animals. This study aims to promote the growth, proliferation, and osteogenic differentiation of mesenchymal stem cells isolated from the adipose tissue of canines on BTCP Betatricalcium phosphate and BTCPCollagen biomaterials. MTT analysis was performed to test the cell adhesion and proliferation on BTCP and BTCPCollagen biomaterials used to mimic the extracellular matrix of threedimensional bone tissue. Scanning electron microscope analysis was performed to show general surface characters of BTCP and BTCP Collagen biomaterials. The osteoinductive capacities of the BTCP and BTCPCollagen biomaterials were determined by alkaline phosphatase and Von Kossa stainings, and RTPCR analysis. The ALP activity of the BTCPCol containing material was significantly higher than the BTCP in the early days. In terms of gene expression, there were no significant differences except 14thday SPARC gene expression. The results of Von Kossa staining indicated that BTCP Col has above the desired level degradation capacity. As a result of this research, although it is advantageous in terms of alkaline phosphatase activity and osteogenic gene expression compared to BTCP material, it is thought that BTCPCollagen biomaterial should be developed for use in bone tissue engineering due to its high degradation property.

Makale detayları

Osteogenic differentiation of canine adipose derived mesenchymal stem cells on B‐TCP and B‐TCP/Collagen biomaterials

Yazarlar:

Özge Özgenç | Asuman Özen

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Histology and Embryology

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 2, pp.125-134

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311976

pISSN:

1300-0861

Adipose tissue

biomaterial

canine

mesenchymal stem cell

osteogenic differentiation.

Sayı

Makale detayları

Osteogenic differentiation of canine adipose derived mesenchymal stem cells on B‐TCP and B‐TCP/Collagen biomaterials

Yazarlar: Özge Özgenç | Asuman Özen

Yayıncı: Ankara University

Ana Başlıklar: Veterinary Histology and Embryology

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 2, pp.125-134

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001311976

pISSN: 1300-0861

Adipose tissue

biomaterial

canine

mesenchymal stem cell

osteogenic differentiation.

Sayı

Yazarlar:

Özge Özgenç | Asuman Özen

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Histology and Embryology

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 2, pp.125-134

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311976

pISSN:

1300-0861