Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry

Fatma Turgut Kaynak; Melek Yanaşik; Suzan Çınar; Sevgi Kalayoğlu Beşışık; Günnur Deniz; Metin Yusuf Gelmez

Makale detayları

Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry

Yazarlar:

Fatma Turgut Kaynak | Melek Yanaşik | Suzan Çınar | Sevgi Kalayoğlu Beşışık | Günnur Deniz | Metin Yusuf Gelmez

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.15 Issue 1, pp.1-14

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348950

pISSN:

2667-5846

Anahtar kelimeler:

Özet:

 Objective: Geographical, age and gender differences possibly affect the distribution of lymphocyte subsets. This study aimed to determine the distribution of T and B lymphocytes and their subsets by evaluating a larger healthy adult population in terms of gender and age. Materials and Methods: Blood samples from 118 healthy adults aged 20-60 years were evaluated using f low cytometry. Results: B cell subsets were not affected by age and gender differences; however, CD3 and CD4 T cell ratios were higher in women than in men. Gender and age differences did not affect the distribution of CD4 T cell subsets, but increased effector memory CD45RACD8 T and decreased naive CD8 T were found among the group aged between 50-60 years. Additionally, regulatory T cells were found at higher levels in individuals aged between 50-60 years. Moreover, high follicular regulatory T cells in men and low follicular helper T cells in women were observed in individuals aged 50-60 years. Advanced age in females may affect the differentiation of CD4 T cells to T helper type 1. Conclusion: Our results indicate that age and gender differences may affect cell distribution. Our findings may contribute to the clinical evaluation of lymphocyte subset distributions as diagnostic tests performed using flow cytometry for early diagnosis and appropriate treatment of patients.

 Objective: Geographical, age and gender differences possibly affect the distribution of lymphocyte subsets. This study aimed to determine the distribution of T and B lymphocytes and their subsets by evaluating a larger healthy adult population in terms of gender and age. Materials and Methods: Blood samples from 118 healthy adults aged 20-60 years were evaluated using f low cytometry. Results: B cell subsets were not affected by age and gender differences; however, CD3 and CD4 T cell ratios were higher in women than in men. Gender and age differences did not affect the distribution of CD4 T cell subsets, but increased effector memory CD45RACD8 T and decreased naive CD8 T were found among the group aged between 50-60 years. Additionally, regulatory T cells were found at higher levels in individuals aged between 50-60 years. Moreover, high follicular regulatory T cells in men and low follicular helper T cells in women were observed in individuals aged 50-60 years. Advanced age in females may affect the differentiation of CD4 T cells to T helper type 1. Conclusion: Our results indicate that age and gender differences may affect cell distribution. Our findings may contribute to the clinical evaluation of lymphocyte subset distributions as diagnostic tests performed using flow cytometry for early diagnosis and appropriate treatment of patients.

 Objective: Geographical, age and gender differences possibly affect the distribution of lymphocyte subsets. This study aimed to determine the distribution of T and B lymphocytes and their subsets by evaluating a larger healthy adult population in terms of gender and age. Materials and Methods: Blood samples from 118 healthy adults aged 20-60 years were evaluated using f low cytometry. Results: B cell subsets were not affected by age and gender differences; however, CD3 and CD4 T cell ratios were higher in women than in men. Gender and age differences did not affect the distribution of CD4 T cell subsets, but increased effector memory CD45RACD8 T and decreased naive CD8 T were found among the group aged between 50-60 years. Additionally, regulatory T cells were found at higher levels in individuals aged between 50-60 years. Moreover, high follicular regulatory T cells in men and low follicular helper T cells in women were observed in individuals aged 50-60 years. Advanced age in females may affect the differentiation of CD4 T cells to T helper type 1. Conclusion: Our results indicate that age and gender differences may affect cell distribution. Our findings may contribute to the clinical evaluation of lymphocyte subset distributions as diagnostic tests performed using flow cytometry for early diagnosis and appropriate treatment of patients.

 Objective: Geographical, age and gender differences possibly affect the distribution of lymphocyte subsets. This study aimed to determine the distribution of T and B lymphocytes and their subsets by evaluating a larger healthy adult population in terms of gender and age. Materials and Methods: Blood samples from 118 healthy adults aged 20-60 years were evaluated using f low cytometry. Results: B cell subsets were not affected by age and gender differences; however, CD3 and CD4 T cell ratios were higher in women than in men. Gender and age differences did not affect the distribution of CD4 T cell subsets, but increased effector memory CD45RACD8 T and decreased naive CD8 T were found among the group aged between 50-60 years. Additionally, regulatory T cells were found at higher levels in individuals aged between 50-60 years. Moreover, high follicular regulatory T cells in men and low follicular helper T cells in women were observed in individuals aged 50-60 years. Advanced age in females may affect the differentiation of CD4 T cells to T helper type 1. Conclusion: Our results indicate that age and gender differences may affect cell distribution. Our findings may contribute to the clinical evaluation of lymphocyte subset distributions as diagnostic tests performed using flow cytometry for early diagnosis and appropriate treatment of patients.

Makale detayları

Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry

Yazarlar:

Fatma Turgut Kaynak | Melek Yanaşik | Suzan Çınar | Sevgi Kalayoğlu Beşışık | Günnur Deniz | Metin Yusuf Gelmez

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.15 Issue 1, pp.1-14

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348950

pISSN:

2667-5846

T lymphocytes

B lymphocytes

Flow cytometry

Reference values of lymphocyte subsets

Sayı

Makale detayları

Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry

Yazarlar: Fatma Turgut Kaynak | Melek Yanaşik | Suzan Çınar | Sevgi Kalayoğlu Beşışık | Günnur Deniz | Metin Yusuf Gelmez

Yayıncı: Istanbul University

Ana Başlıklar: Clinical Sciences (Other)

Dergi: Experimed Vol.15 Issue 1, pp.1-14

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001348950

pISSN: 2667-5846

T lymphocytes

B lymphocytes

Flow cytometry

Reference values of lymphocyte subsets

Sayı

Yazarlar:

Fatma Turgut Kaynak | Melek Yanaşik | Suzan Çınar | Sevgi Kalayoğlu Beşışık | Günnur Deniz | Metin Yusuf Gelmez

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.15 Issue 1, pp.1-14

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348950

pISSN:

2667-5846