The protective and antiapoptotic effects of Hypericum triquetrifolium Turra against cyclophosphamide-induced lung injury in rats: in vitro evaluation

Songül Çetik Yıldız; Cumali Keskin; Varol Şahintürk; Adnan Ayhancı

Makale detayları

The protective and antiapoptotic effects of Hypericum triquetrifolium Turra against cyclophosphamide-induced lung injury in rats: in vitro evaluation

Yazarlar:

Songül Çetik Yıldız | Cumali Keskin | Varol Şahintürk | Adnan Ayhancı

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Cell Metabolism | Systems Biology | Structural Biology | Biochemistry and Cell Biology (Other)

Dergi:

The European Research Journal Vol.10 Issue 2, pp.157-165

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311828

pISSN:

2149-3189

Anahtar kelimeler:

Özet:

 Objectives: Hypericum triquetrifolium Turra HTT has been traditionally used in medical treatments due to its sedative, antiseptic, antiinflammatory, and anthelmintic properties. The present study aims to investigate the lung-protective and antiapoptotic effects of HTT against cyclophosphamide CP-induced lung injury in rats. Methods: Thirty-five Sprague Dawley rats were categorized into 5 groups, each consisting of seven members. Phenolic acid and flavonoid contents of this plant were determined. The lung tissue samples cultivated from the rats were examined in histopathological and immunohistochemically for the apoptosis markers of Caspase-3, Bax, and Bcl-2. Results: Histopathological results indicated that structural defects, bleeding areas, and edema had occurred in the lungs of the CP-Alone Group. Besides, Caspase-3 and Bax positivity of the lung cells had also increased while Bcl-2 positivity had decreased. On the other hand, in the HTTCP Group, HTT was shown to have reversed the aforementioned changes positively. Conclusion: Based on in vivo results, HTT could be a strong protective candidate for CP-induced lung injury and apoptosis.

 Objectives: Hypericum triquetrifolium Turra HTT has been traditionally used in medical treatments due to its sedative, antiseptic, antiinflammatory, and anthelmintic properties. The present study aims to investigate the lung-protective and antiapoptotic effects of HTT against cyclophosphamide CP-induced lung injury in rats. Methods: Thirty-five Sprague Dawley rats were categorized into 5 groups, each consisting of seven members. Phenolic acid and flavonoid contents of this plant were determined. The lung tissue samples cultivated from the rats were examined in histopathological and immunohistochemically for the apoptosis markers of Caspase-3, Bax, and Bcl-2. Results: Histopathological results indicated that structural defects, bleeding areas, and edema had occurred in the lungs of the CP-Alone Group. Besides, Caspase-3 and Bax positivity of the lung cells had also increased while Bcl-2 positivity had decreased. On the other hand, in the HTTCP Group, HTT was shown to have reversed the aforementioned changes positively. Conclusion: Based on in vivo results, HTT could be a strong protective candidate for CP-induced lung injury and apoptosis.

 Objectives: Hypericum triquetrifolium Turra HTT has been traditionally used in medical treatments due to its sedative, antiseptic, antiinflammatory, and anthelmintic properties. The present study aims to investigate the lung-protective and antiapoptotic effects of HTT against cyclophosphamide CP-induced lung injury in rats. Methods: Thirty-five Sprague Dawley rats were categorized into 5 groups, each consisting of seven members. Phenolic acid and flavonoid contents of this plant were determined. The lung tissue samples cultivated from the rats were examined in histopathological and immunohistochemically for the apoptosis markers of Caspase-3, Bax, and Bcl-2. Results: Histopathological results indicated that structural defects, bleeding areas, and edema had occurred in the lungs of the CP-Alone Group. Besides, Caspase-3 and Bax positivity of the lung cells had also increased while Bcl-2 positivity had decreased. On the other hand, in the HTTCP Group, HTT was shown to have reversed the aforementioned changes positively. Conclusion: Based on in vivo results, HTT could be a strong protective candidate for CP-induced lung injury and apoptosis.

 Objectives: Hypericum triquetrifolium Turra HTT has been traditionally used in medical treatments due to its sedative, antiseptic, antiinflammatory, and anthelmintic properties. The present study aims to investigate the lung-protective and antiapoptotic effects of HTT against cyclophosphamide CP-induced lung injury in rats. Methods: Thirty-five Sprague Dawley rats were categorized into 5 groups, each consisting of seven members. Phenolic acid and flavonoid contents of this plant were determined. The lung tissue samples cultivated from the rats were examined in histopathological and immunohistochemically for the apoptosis markers of Caspase-3, Bax, and Bcl-2. Results: Histopathological results indicated that structural defects, bleeding areas, and edema had occurred in the lungs of the CP-Alone Group. Besides, Caspase-3 and Bax positivity of the lung cells had also increased while Bcl-2 positivity had decreased. On the other hand, in the HTTCP Group, HTT was shown to have reversed the aforementioned changes positively. Conclusion: Based on in vivo results, HTT could be a strong protective candidate for CP-induced lung injury and apoptosis.

Makale detayları

The protective and antiapoptotic effects of Hypericum triquetrifolium Turra against cyclophosphamide-induced lung injury in rats: in vitro evaluation

Yazarlar:

Songül Çetik Yıldız | Cumali Keskin | Varol Şahintürk | Adnan Ayhancı

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Cell Metabolism | Systems Biology | Structural Biology | Biochemistry and Cell Biology (Other)

Dergi:

The European Research Journal Vol.10 Issue 2, pp.157-165

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311828

pISSN:

2149-3189

Cyclophosphamide

Hypericum triquetrifolium

immunohistochemical

apoptosis

rats

Sayı

Makale detayları

The protective and antiapoptotic effects of Hypericum triquetrifolium Turra against cyclophosphamide-induced lung injury in rats: in vitro evaluation

Yazarlar: Songül Çetik Yıldız | Cumali Keskin | Varol Şahintürk | Adnan Ayhancı

Yayıncı: Prusa Medical Publishing

Ana Başlıklar: Cell Metabolism | Systems Biology | Structural Biology | Biochemistry and Cell Biology (Other)

Dergi: The European Research Journal Vol.10 Issue 2, pp.157-165

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001311828

pISSN: 2149-3189

Cyclophosphamide

Hypericum triquetrifolium

immunohistochemical

apoptosis

rats

Sayı

Yazarlar:

Songül Çetik Yıldız | Cumali Keskin | Varol Şahintürk | Adnan Ayhancı

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Cell Metabolism | Systems Biology | Structural Biology | Biochemistry and Cell Biology (Other)

Dergi:

The European Research Journal Vol.10 Issue 2, pp.157-165

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311828

pISSN:

2149-3189