Screening of Phytochemical Constituents in Ethanolic Extract from Astragalus spinosus Roots and Assessment of Its Anticancer, Antioxidant, and Antibacterial Activities

Ahmed Abdulrazzaq Hadi; Ameena Naeem Seeewan; Zina Abdulhussein Jawad; Ali Yahya Naoom; Rusul Ridha Abdulmahdi

Makale detayları

Screening of Phytochemical Constituents in Ethanolic Extract from Astragalus spinosus Roots and Assessment of Its Anticancer, Antioxidant, and Antibacterial Activities

Yazarlar:

Ahmed Abdulrazzaq Hadi | Ameena Naeem Seeewan | Zina Abdulhussein Jawad | Ali Yahya Naoom | Rusul Ridha Abdulmahdi

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Separation Science

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 1, pp.149-160

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001303740

pISSN:

2149-0120

Anahtar kelimeler:

Özet:

 The study aims to analyze the chemical components of Astragalus spinosus roots extract after the ethanolic extraction process using techniques such as gas chromatography-mass spectrometry, infrared, and UV-Vis spectroscopy to indicate the phytochemical content of the extract. The extract contained various compounds, including alkaloids, terpenoids, flavonoids, phenols, and carboxylic acids. Flavonoid and phenolic content were measured by the colorimetric method and found to be 511.1935.75 and 24.640.07 mg, respectively. Subsequently, antibacterial, antioxidant, and anticancer activities were evaluated for the ethanolic extract. Antibacterial effectiveness was concentration-dependent against Staphylococcus aureus and Streptococcus mutans, showing optimal inhibition at 100 mgmL with inhibition areas of 2.810.88 and 2.682.93 mm, respectively. Antioxidant activity was measured using DPPH with a 200 µgmL of extract concentration, displaying maximum scavenging activity 71.856.43 and FRAP activity 55.931.4 at 0.64 µgmL. The alcoholic extract exhibited decreasing vitality of MCF-7 breast cancer cells as concentration increased, with viability reaching a minimum of 35.378  5.072 at 400 μgmL.

 The study aims to analyze the chemical components of Astragalus spinosus roots extract after the ethanolic extraction process using techniques such as gas chromatography-mass spectrometry, infrared, and UV-Vis spectroscopy to indicate the phytochemical content of the extract. The extract contained various compounds, including alkaloids, terpenoids, flavonoids, phenols, and carboxylic acids. Flavonoid and phenolic content were measured by the colorimetric method and found to be 511.1935.75 and 24.640.07 mg, respectively. Subsequently, antibacterial, antioxidant, and anticancer activities were evaluated for the ethanolic extract. Antibacterial effectiveness was concentration-dependent against Staphylococcus aureus and Streptococcus mutans, showing optimal inhibition at 100 mgmL with inhibition areas of 2.810.88 and 2.682.93 mm, respectively. Antioxidant activity was measured using DPPH with a 200 µgmL of extract concentration, displaying maximum scavenging activity 71.856.43 and FRAP activity 55.931.4 at 0.64 µgmL. The alcoholic extract exhibited decreasing vitality of MCF-7 breast cancer cells as concentration increased, with viability reaching a minimum of 35.378  5.072 at 400 μgmL.

 The study aims to analyze the chemical components of Astragalus spinosus roots extract after the ethanolic extraction process using techniques such as gas chromatography-mass spectrometry, infrared, and UV-Vis spectroscopy to indicate the phytochemical content of the extract. The extract contained various compounds, including alkaloids, terpenoids, flavonoids, phenols, and carboxylic acids. Flavonoid and phenolic content were measured by the colorimetric method and found to be 511.1935.75 and 24.640.07 mg, respectively. Subsequently, antibacterial, antioxidant, and anticancer activities were evaluated for the ethanolic extract. Antibacterial effectiveness was concentration-dependent against Staphylococcus aureus and Streptococcus mutans, showing optimal inhibition at 100 mgmL with inhibition areas of 2.810.88 and 2.682.93 mm, respectively. Antioxidant activity was measured using DPPH with a 200 µgmL of extract concentration, displaying maximum scavenging activity 71.856.43 and FRAP activity 55.931.4 at 0.64 µgmL. The alcoholic extract exhibited decreasing vitality of MCF-7 breast cancer cells as concentration increased, with viability reaching a minimum of 35.378  5.072 at 400 μgmL.

 The study aims to analyze the chemical components of Astragalus spinosus roots extract after the ethanolic extraction process using techniques such as gas chromatography-mass spectrometry, infrared, and UV-Vis spectroscopy to indicate the phytochemical content of the extract. The extract contained various compounds, including alkaloids, terpenoids, flavonoids, phenols, and carboxylic acids. Flavonoid and phenolic content were measured by the colorimetric method and found to be 511.1935.75 and 24.640.07 mg, respectively. Subsequently, antibacterial, antioxidant, and anticancer activities were evaluated for the ethanolic extract. Antibacterial effectiveness was concentration-dependent against Staphylococcus aureus and Streptococcus mutans, showing optimal inhibition at 100 mgmL with inhibition areas of 2.810.88 and 2.682.93 mm, respectively. Antioxidant activity was measured using DPPH with a 200 µgmL of extract concentration, displaying maximum scavenging activity 71.856.43 and FRAP activity 55.931.4 at 0.64 µgmL. The alcoholic extract exhibited decreasing vitality of MCF-7 breast cancer cells as concentration increased, with viability reaching a minimum of 35.378  5.072 at 400 μgmL.

Makale detayları

Screening of Phytochemical Constituents in Ethanolic Extract from Astragalus spinosus Roots and Assessment of Its Anticancer, Antioxidant, and Antibacterial Activities

Yazarlar:

Ahmed Abdulrazzaq Hadi | Ameena Naeem Seeewan | Zina Abdulhussein Jawad | Ali Yahya Naoom | Rusul Ridha Abdulmahdi

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Separation Science

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 1, pp.149-160

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001303740

pISSN:

2149-0120

Astragalus spinosus

Chemical components

GC-mass

Antioxidant activity

Antibacterial inhibition

Anticancer effect.

Sayı

Makale detayları

Screening of Phytochemical Constituents in Ethanolic Extract from Astragalus spinosus Roots and Assessment of Its Anticancer, Antioxidant, and Antibacterial Activities

Yazarlar: Ahmed Abdulrazzaq Hadi | Ameena Naeem Seeewan | Zina Abdulhussein Jawad | Ali Yahya Naoom | Rusul Ridha Abdulmahdi

Yayıncı: Turkish Chemical Society

Ana Başlıklar: Separation Science

Dergi: Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 1, pp.149-160

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001303740

pISSN: 2149-0120

Astragalus spinosus

Chemical components

GC-mass

Antioxidant activity

Antibacterial inhibition

Anticancer effect.

Sayı

Yazarlar:

Ahmed Abdulrazzaq Hadi | Ameena Naeem Seeewan | Zina Abdulhussein Jawad | Ali Yahya Naoom | Rusul Ridha Abdulmahdi

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Separation Science

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 1, pp.149-160

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001303740

pISSN:

2149-0120