Effect of dietary β-sitosterol on fecal bacterial and colonic biotransformation enzymes in 1,2-dimethylhydrazine–induced colon carcinogenesis

Albert Baskar ARUL; Khalid AL NUMAIR; Mohammed AL SAIF; Ignacimuthu SAVARIMUTHU

Makale detayları

Effect of dietary β-sitosterol on fecal bacterial and colonic biotransformation enzymes in 1,2-dimethylhydrazine–induced colon carcinogenesis

Yazarlar:

Albert Baskar ARUL | Khalid AL NUMAIR | Mohammed AL SAIF | Ignacimuthu SAVARIMUTHU

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue Sup.1, pp.1307-1313

Belge Tipi:

Makale

Yayın Tarihi:

11/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000754389

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

The present study was designed to investigate the modulatory effect of b-sitosterol administration on 1,2-dimethylhydrazine (DMH)-induced fecal bacterial and colonic biotransformation enzyme activities.   Materials and methods: The chemopreventive potential of b-sitosterol in colon carcinogenesis was assessed by injecting DMH (20 mg/kg body weight) subcutaneously into male Wistar rats and supplementing with b-sitosterol throughout the experimental period of 16 weeks at 3 different doses (5, 10, and 20 mg/kg body weight).   Results: Amplified activities of fecal bacterial (b-glucuronidase, b-glucosidase, b-galactosidase, nitroreductase, hyaluronate lyase, and sulfatase) and colonic biotransformation (b-glucuronidase, b-glucosidase, b-galactosidase, and nitroreductase) enzymes were considered hallmarks of colon carcinogenesis. DMH-induced animals showed increased activities of fecal bacterial and colonic biotransformation enzymes. Treatment with b-sitosterol markedly decreased the fecal bacterial and colonic biotransformation enzymes and reverted the colonic tissue to near normal.   Conclusion: The results of the present study revealed that b-sitosterol markedly inhibited DMH-induced colon carcinogenesis by its ability to ameliorate the fecal bacterial and colonic biotransformation enzymes. Hence, b-sitosterol can be a potential chemopreventive agent towards colon carcinogenesis.

 

The present study was designed to investigate the modulatory effect of b-sitosterol administration on 1,2-dimethylhydrazine (DMH)-induced fecal bacterial and colonic biotransformation enzyme activities.   Materials and methods: The chemopreventive potential of b-sitosterol in colon carcinogenesis was assessed by injecting DMH (20 mg/kg body weight) subcutaneously into male Wistar rats and supplementing with b-sitosterol throughout the experimental period of 16 weeks at 3 different doses (5, 10, and 20 mg/kg body weight).   Results: Amplified activities of fecal bacterial (b-glucuronidase, b-glucosidase, b-galactosidase, nitroreductase, hyaluronate lyase, and sulfatase) and colonic biotransformation (b-glucuronidase, b-glucosidase, b-galactosidase, and nitroreductase) enzymes were considered hallmarks of colon carcinogenesis. DMH-induced animals showed increased activities of fecal bacterial and colonic biotransformation enzymes. Treatment with b-sitosterol markedly decreased the fecal bacterial and colonic biotransformation enzymes and reverted the colonic tissue to near normal.   Conclusion: The results of the present study revealed that b-sitosterol markedly inhibited DMH-induced colon carcinogenesis by its ability to ameliorate the fecal bacterial and colonic biotransformation enzymes. Hence, b-sitosterol can be a potential chemopreventive agent towards colon carcinogenesis.

Makale detayları

Effect of dietary β-sitosterol on fecal bacterial and colonic biotransformation enzymes in 1,2-dimethylhydrazine–induced colon carcinogenesis

Yazarlar:

Albert Baskar ARUL | Khalid AL NUMAIR | Mohammed AL SAIF | Ignacimuthu SAVARIMUTHU

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue Sup.1, pp.1307-1313

Belge Tipi:

Makale

Yayın Tarihi:

11/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000754389

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: b-Sitosterol

b-glucuronidase

b-glucosidase

b-galactosidase

nitroreductase

hyaluronate lyase

sulfatase

Sayı

Makale detayları

Yazarlar: Albert Baskar ARUL | Khalid AL NUMAIR | Mohammed AL SAIF | Ignacimuthu SAVARIMUTHU

Yayıncı: TUBITAK

Ana Başlıklar: Sağlık teşvikleri

Dergi: Turkish Journal of Medical Sciences 2012 Vol.42 Issue Sup.1, pp.1307-1313

Belge Tipi: Makale

Yayın Tarihi: 11/2011

Belge Dili: İngilizce

Document ID (Org No.): 2000754389

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Albert Baskar ARUL | Khalid AL NUMAIR | Mohammed AL SAIF | Ignacimuthu SAVARIMUTHU

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue Sup.1, pp.1307-1313

Belge Tipi:

Makale

Yayın Tarihi:

11/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000754389

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144